ID   KOSYN_HERA2             Reviewed;         289 AA.
AC   A9AWD6;
DT   25-APR-2018, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 1.
DT   03-AUG-2022, entry version 48.
DE   RecName: Full=(+)-kolavelool synthase {ECO:0000303|PubMed:25694050};
DE            EC=3.1.7.12 {ECO:0000269|PubMed:25694050};
GN   OrderedLocusNames=Haur_2146 {ECO:0000312|EMBL:ABX04786.1};
OS   Herpetosiphon aurantiacus (strain ATCC 23779 / DSM 785 / 114-95).
OC   Bacteria; Chloroflexi; Chloroflexia; Herpetosiphonales; Herpetosiphonaceae;
OC   Herpetosiphon.
OX   NCBI_TaxID=316274;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 23779 / DSM 785 / 114-95 {ECO:0000312|Proteomes:UP000000787};
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C.,
RA   Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.,
RA   Bryant D.A., Richardson P.;
RT   "Complete sequence of chromosome of Herpetosiphon aurantiacus ATCC 23779.";
RL   Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=ATCC 23779 / DSM 785 / 114-95;
RX   PubMed=25694050; DOI=10.1002/cbic.201402652;
RA   Nakano C., Oshima M., Kurashima N., Hoshino T.;
RT   "Identification of a new diterpene biosynthetic gene cluster that produces
RT   O-methylkolavelool in Herpetosiphon aurantiacus.";
RL   ChemBioChem 16:772-781(2015).
CC   -!- FUNCTION: Involved in the biosynthesis of (+)-O-methylkolavelool.
CC       Catalyzes the biosynthesis of (+)-kolavelool from (+)-kolavenyl
CC       diphosphate via the release of the diphosphate moiety through the
CC       nucleophilic addition of a water molecule.
CC       {ECO:0000269|PubMed:25694050}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(+)-kolavenyl diphosphate + H2O = (+)-kolavelool +
CC         diphosphate; Xref=Rhea:RHEA:54672, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:138311, ChEBI:CHEBI:138313;
CC         EC=3.1.7.12; Evidence={ECO:0000269|PubMed:25694050};
CC   -!- SIMILARITY: Belongs to the diterpene synthase family. {ECO:0000305}.
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DR   EMBL; CP000875; ABX04786.1; -; Genomic_DNA.
DR   AlphaFoldDB; A9AWD6; -.
DR   SMR; A9AWD6; -.
DR   STRING; 316274.Haur_2146; -.
DR   EnsemblBacteria; ABX04786; ABX04786; Haur_2146.
DR   KEGG; hau:Haur_2146; -.
DR   eggNOG; ENOG502ZJEY; Bacteria.
DR   HOGENOM; CLU_962320_0_0_0; -.
DR   BioCyc; HAUR316274:GHYA-2174-MON; -.
DR   BRENDA; 3.1.7.12; 2656.
DR   Proteomes; UP000000787; Chromosome.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016765; F:transferase activity, transferring alkyl or aryl (other than methyl) groups; IEA:InterPro.
DR   Gene3D; 3.40.1180.10; -; 1.
DR   InterPro; IPR036424; UPP_synth-like_sf.
PE   1: Evidence at protein level;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..289
FT                   /note="(+)-kolavelool synthase"
FT                   /id="PRO_0000443952"
SQ   SEQUENCE   289 AA;  33593 MW;  3810389A368B086D CRC64;
     MRPTPTLAEF LHAPLTTIRQ VAPATMVFSS GGSRRKAALA NMSAAGEEYA RWSHQQLLKC
     LELFFSHGIK HLFLPMLLPN QFQETTPNYR EHIEQWVAWG AASQTMLEYY QEHNWRVRLL
     DTQYSPILAD AAQRLQQPYD HPDQPTLWWF VVRDSEDPWQ IIFQAAQKTV FKTRSQAIEA
     IYGEPIPPAE LFVSFGKPQV NHDLLPPLLV GELQCYWTQK PGYTLSEEEF RQILYDFAFL
     RKTWQVDKTE RTQAALAFRQ HWERGPILGL GQQLGPFWYP QSTSIESEL