KPBA_DROME
ID KPBA_DROME Reviewed; 1247 AA.
AC Q9W391; Q86B60; Q95TZ6;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2003, sequence version 2.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Probable phosphorylase b kinase regulatory subunit alpha;
DE Short=Phosphorylase kinase subunit alpha;
GN ORFNames=CG7766;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 148-1247 (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1030 AND SER-1033, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Phosphorylase b kinase catalyzes the phosphorylation of
CC serine in certain substrates, including troponin I. The alpha chain may
CC bind calmodulin (By similarity). {ECO:0000250}.
CC -!- PATHWAY: Glycan biosynthesis; glycogen metabolism.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}; Cytoplasmic side {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A;
CC IsoId=Q9W391-1; Sequence=Displayed;
CC Name=B;
CC IsoId=Q9W391-2; Sequence=VSP_010298;
CC -!- PTM: Although the final Cys may be farnesylated, the terminal
CC tripeptide is probably not removed, and the C-terminus is not
CC methylated. {ECO:0000250|UniProtKB:P18688}.
CC -!- SIMILARITY: Belongs to the phosphorylase b kinase regulatory chain
CC family. {ECO:0000305}.
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DR EMBL; AE014298; AAO41642.2; -; Genomic_DNA.
DR EMBL; AE014298; AAF46443.4; -; Genomic_DNA.
DR EMBL; AY058417; AAL13646.1; -; mRNA.
DR RefSeq; NP_572525.3; NM_132297.6. [Q9W391-1]
DR RefSeq; NP_788889.2; NM_176716.4. [Q9W391-2]
DR AlphaFoldDB; Q9W391; -.
DR SMR; Q9W391; -.
DR BioGRID; 58294; 11.
DR IntAct; Q9W391; 4.
DR iPTMnet; Q9W391; -.
DR PaxDb; Q9W391; -.
DR PRIDE; Q9W391; -.
DR DNASU; 31839; -.
DR EnsemblMetazoa; FBtr0071351; FBpp0071286; FBgn0030087. [Q9W391-1]
DR EnsemblMetazoa; FBtr0071352; FBpp0071287; FBgn0030087. [Q9W391-2]
DR GeneID; 31839; -.
DR KEGG; dme:Dmel_CG7766; -.
DR UCSC; CG7766-RA; d. melanogaster. [Q9W391-1]
DR FlyBase; FBgn0030087; CG7766.
DR VEuPathDB; VectorBase:FBgn0030087; -.
DR eggNOG; KOG3635; Eukaryota.
DR GeneTree; ENSGT00950000183118; -.
DR InParanoid; Q9W391; -.
DR PhylomeDB; Q9W391; -.
DR Reactome; R-DME-70221; Glycogen breakdown (glycogenolysis).
DR UniPathway; UPA00163; -.
DR BioGRID-ORCS; 31839; 0 hits in 3 CRISPR screens.
DR ChiTaRS; CG7766; fly.
DR GenomeRNAi; 31839; -.
DR PRO; PR:Q9W391; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0030087; Expressed in adult hindgut (Drosophila) and 34 other tissues.
DR ExpressionAtlas; Q9W391; baseline and differential.
DR Genevisible; Q9W391; DM.
DR GO; GO:0005964; C:phosphorylase kinase complex; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0005977; P:glycogen metabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR011613; GH15-like.
DR InterPro; IPR045583; KPBA/B_C.
DR InterPro; IPR008734; PHK_A/B_su.
DR PANTHER; PTHR10749; PTHR10749; 1.
DR Pfam; PF00723; Glyco_hydro_15; 1.
DR Pfam; PF19292; KPBB_C; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Calmodulin-binding; Carbohydrate metabolism;
KW Cell membrane; Glycogen metabolism; Lipoprotein; Membrane; Phosphoprotein;
KW Prenylation; Reference proteome.
FT CHAIN 1..1247
FT /note="Probable phosphorylase b kinase regulatory subunit
FT alpha"
FT /id="PRO_0000057735"
FT REGION 853..883
FT /note="Calmodulin-binding"
FT /evidence="ECO:0000255"
FT REGION 1052..1089
FT /note="Calmodulin-binding"
FT /evidence="ECO:0000255"
FT MOD_RES 1030
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1033
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT LIPID 1244
FT /note="S-farnesyl cysteine"
FT /evidence="ECO:0000250|UniProtKB:P18688"
FT VAR_SEQ 524..570
FT /note="FADLSRFYIASDNELMIDILKGEINFLKSAWDLLGRPLVTLVLKRIH -> H
FT IDYEEYYTTRDPDLLASNFTTNLAFLTNNWRHMLGRPTITLMATHYM (in isoform
FT B)"
FT /evidence="ECO:0000305"
FT /id="VSP_010298"
SQ SEQUENCE 1247 AA; 140512 MW; BE9EAAC40C68D72E CRC64;
MRSRSNSGVR LDYYQRIVHR LILAHQEPVT GLFPASNVNS HAWIRDNVYC ILAVWGLSMA
YKKIADQDED RAKCYELEQS CVKLMRGLLM AMMNQKDKVE KFKMTQSPYD SLHAKYSSKN
GLPVVDDNEW GHLQIDAVSL YLLILAQMTA SGLQIVFSLD EVSFIQNLVF YIESAYSIPD
YGIWERGDKT NHGEPELNAS SIGMAKAALE AMNELDLFGA RGGPASVIHV LADEAHKCQA
VLQSMLPRES NSKELDSGLL CVIGFPAFAV DDAQLIHNTK DAILSRLQGK YGCKRFLRDG
YRTPKEDPSR LYYERWELRM FENIECEWPL FYCYLILFHA FQSDKRAVEE YASRLEKIMV
RSEDGILLVP ESYAVPQDLV GFEYQKPGSQ VREVVGRCPF LWGQSLFILG RLLQEGFLAV
GELDPLNRRL GAQKKPDVVV QVVIIAEDNE IRDKLAEHDL HVQTIAEVAP IEVQPARVLS
HLYTYLGRNR KLGLSGRKSR DVGILSTSKL YSLKDRIFAF TPQFADLSRF YIASDNELMI
DILKGEINFL KSAWDLLGRP LVTLVLKRIH LDQDKIPLAM IQTMRKLKSG YINGTRVMLG
SLKDFLNTSA ITDLSFLGST EDGYPDRLHP DVQTYLDEHL LRSFSNRSTM NLRGGQLRPR
TLRRRMSCKG AIKKTRSINV DSDNLGMEGP SPLTERRLSS IVPPPWLQAN KQSHVSVFAT
TPEEGPTSSP LSLGNDLIRE NIYPVDPHHS RSAIDRRSEF VRQQEMPKIL IQRHRAETNF
ADTEVEELIA MLRETENLEE QGDILQYLVD TQGLDFNTAG LGFKNKSEEN ATPNANNAGM
LEEGRVVTVR DLLKGLYEKA CQQKLWGLVR HTAGMLGKRV EDLAKAVTDL LVRQKQVTVG
MPPNNEHTIT APLPEVELRQ LIHDAYGDDE STAMLTQELM VYLAMFIRTE PQLFHEMLRL
RVGLIIQVMA KELSRTLNCD GEAASEHLLN LSPFEMKNLL YHILSGKEFA VSSVARGNLS
IVSCKSSRVS KKSQIGLGDP EGEDALIATI DDRQGQWLRR RRLDGALNRV PRDFYSRVWT
VLEKCQGLAI EGRVLQQSLT QEMTPGELKF ALEVETALNQ IPQPEYRQLV VEALMVLTLV
TEHNMVPSLG GVIYVEHLVH KANQLFLEDQ RKVQGDATLC CAKIKDGKEQ QQAASGMLLC
GGAAYICQHL YDSAPSGSYG TMTYMSRAVA LVLDCVPKHG EMECAIS
