KPBB_DROME
ID KPBB_DROME Reviewed; 1093 AA.
AC Q9VLS1; F9VMG4; Q8IPG3; Q8SXP4;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2003, sequence version 2.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Probable phosphorylase b kinase regulatory subunit beta;
DE Short=Phosphorylase kinase subunit beta;
GN ORFNames=CG8475;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP SEQUENCE REVISION.
RC STRAIN=Berkeley;
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Phosphorylase b kinase catalyzes the phosphorylation of
CC serine in certain substrates, including troponin I. The beta chain acts
CC as a regulatory unit and modulates the activity of the holoenzyme in
CC response to phosphorylation (By similarity). {ECO:0000250}.
CC -!- PATHWAY: Glycan biosynthesis; glycogen metabolism.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}; Cytoplasmic side {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A;
CC IsoId=Q9VLS1-1; Sequence=Displayed;
CC Name=B;
CC IsoId=Q9VLS1-2; Sequence=VSP_007911, VSP_007912;
CC -!- PTM: Although the final Cys may be farnesylated, the terminal
CC tripeptide is probably not removed, and the C-terminus is not
CC methylated. {ECO:0000250|UniProtKB:P12798}.
CC -!- SIMILARITY: Belongs to the phosphorylase b kinase regulatory chain
CC family. {ECO:0000305}.
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DR EMBL; AE014134; AAF52613.2; -; Genomic_DNA.
DR EMBL; AE014134; AAN11154.1; -; Genomic_DNA.
DR EMBL; AY089501; AEM24976.1; -; mRNA.
DR RefSeq; NP_609189.2; NM_135345.3. [Q9VLS1-1]
DR RefSeq; NP_723356.1; NM_164797.3. [Q9VLS1-2]
DR AlphaFoldDB; Q9VLS1; -.
DR BioGRID; 60243; 11.
DR IntAct; Q9VLS1; 2.
DR STRING; 7227.FBpp0079254; -.
DR PaxDb; Q9VLS1; -.
DR EnsemblMetazoa; FBtr0079638; FBpp0079254; FBgn0031995. [Q9VLS1-1]
DR EnsemblMetazoa; FBtr0079639; FBpp0079255; FBgn0031995. [Q9VLS1-2]
DR GeneID; 34114; -.
DR KEGG; dme:Dmel_CG8475; -.
DR UCSC; CG8475-RA; d. melanogaster. [Q9VLS1-1]
DR FlyBase; FBgn0031995; CG8475.
DR VEuPathDB; VectorBase:FBgn0031995; -.
DR eggNOG; KOG3635; Eukaryota.
DR GeneTree; ENSGT00950000183118; -.
DR HOGENOM; CLU_004177_0_1_1; -.
DR InParanoid; Q9VLS1; -.
DR PhylomeDB; Q9VLS1; -.
DR Reactome; R-DME-70221; Glycogen breakdown (glycogenolysis).
DR UniPathway; UPA00163; -.
DR BioGRID-ORCS; 34114; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 34114; -.
DR PRO; PR:Q9VLS1; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0031995; Expressed in mouthpart and 26 other tissues.
DR ExpressionAtlas; Q9VLS1; baseline and differential.
DR Genevisible; Q9VLS1; DM.
DR GO; GO:0005964; C:phosphorylase kinase complex; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0005977; P:glycogen metabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR011613; GH15-like.
DR InterPro; IPR045583; KPBA/B_C.
DR InterPro; IPR008734; PHK_A/B_su.
DR PANTHER; PTHR10749; PTHR10749; 1.
DR Pfam; PF00723; Glyco_hydro_15; 1.
DR Pfam; PF19292; KPBB_C; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Calmodulin-binding; Carbohydrate metabolism;
KW Cell membrane; Glycogen metabolism; Lipoprotein; Membrane; Prenylation;
KW Reference proteome.
FT CHAIN 1..1093
FT /note="Probable phosphorylase b kinase regulatory subunit
FT beta"
FT /id="PRO_0000057739"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 6..27
FT /note="Calmodulin-binding"
FT /evidence="ECO:0000255"
FT REGION 751..778
FT /note="Calmodulin-binding"
FT /evidence="ECO:0000255"
FT REGION 905..936
FT /note="Calmodulin-binding"
FT /evidence="ECO:0000255"
FT COMPBIAS 11..25
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 1090
FT /note="S-farnesyl cysteine"
FT /evidence="ECO:0000250|UniProtKB:P12798"
FT VAR_SEQ 475..506
FT /note="GTATDLVVQIVLIAESMRLQAMMATYGIQTQT -> VSWNRDSWRALSAMKC
FT FLKLLTNGKAFWITIG (in isoform B)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_007911"
FT VAR_SEQ 507..1093
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_007912"
SQ SEQUENCE 1093 AA; 124864 MW; B2555E808834E9BF CRC64;
MRDVPKSLGL SVTTPGGSSG APDSGRHNSL EEINLDQFLK TSNYEDTVKQ LDIYYGIVKR
QLLRYQSPIT GLFPVMSTDQ VVGSVRDSVY CASAVWSLYQ AYRRIDDDRG KSYELGQSTV
KCMRGILECW VKQASRVELF KQRQSNQHAL HSKFQLHTGE KIYPDEFYNH LQIDCVSLYL
LFLVQMITSG LQIIYTHDEV AFVQNLVYYV ERAYRTPDFG MWERGSKYNN GTPEIHASSI
GMAKSALEAI NGCNLFGEKG ASWSVVYVDI DAHNRNRSIF ETMLPRESSS KGVDASLLLT
LSFPAFASHE DRLVEQTKQN VVNRLRCKMG FKRFTRDGFL SKNEDKSRRY YHSGELKEFE
GLECEWPLFF IAMIIDGVFK NNNEQIEEFQ NDLRRCLRTD VNGDPVVTMY YAPDGDGSYM
RAPSQSLFLW GQSFFIIAQL LTAGLLHINE LDPIRRYLPS YNRPRRAGRY SAFQGTATDL
VVQIVLIAES MRLQAMMATY GIQTQTPHEV EPVQIWSSTE LIKVYQHLGV NNKVGLSGRP
CRPVGSLGTS KVYRICGMTV LCYPLIFEVS DFYLYRDMAL LIDDIKTELQ FVGKYWRLSG
RPTVCLLIRE EHMRDPQFKE MLDLLAMLKK GYCDGMKVRI GRLQNLISSS CIEHLDFMNQ
SDLTDNENAF SQINHEYIGY QSLTDVPKAL TYVEEKISVA HFDTKPTPDI INALRSTDSI
YCLCQLWGII LNREGPHFEV NGLNVNTALT QLYHRAGSLR YWRAVRYCSS LLHHIVDSIS
PFITTVLVNG KELTVGIIGQ KETVFDKPMT PAEIQNVMYT SVQPYDVIQA VLQQEVVLYC
GRLIATNPSM FRGILKIRIG WVLEAMRIYL QISGQQSIDV DNLSPFQVRI LLQKVLTVSE
WAVEEKLTTL QRRQLEGCLC RVPKHFYNKI WEILQRTPQG ILTQGHHLPA TPTLTNMSRG
ELTFNLLVEE TLICIDRPER RQITVELLCI VATILNRNPE LHFKQALDLD GILAEAFAMY
CKDNNIQHQP KPEHEQTKNE DLKAFYSLPY SETTGYLARA AVNKVLQGGI FSTNEEDVQL
DGDRLHDDNC KVS
