ID   KPL_ARATH               Reviewed;         492 AA.
AC   Q9FFP2; Q5BPF0;
DT   10-MAY-2017, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Protein KOKOPELLI {ECO:0000303|PubMed:20478994};
GN   Name=KPL {ECO:0000303|PubMed:20478994};
GN   OrderedLocusNames=At5g63720 {ECO:0000312|Araport:AT5G63720};
GN   ORFNames=MBK5.20 {ECO:0000312|EMBL:BAB10465.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA   Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA   Miyajima N., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT   features of the 1.6 Mb regions covered by twenty physically assigned P1
RT   clones.";
RL   DNA Res. 4:215-230(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 246-492.
RC   STRAIN=cv. Columbia;
RA   Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA   Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL   Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP   STAGE.
RC   STRAIN=cv. Landsberg erecta, and cv. Wassilewskija-4;
RX   PubMed=20478994; DOI=10.1101/gad.1882810;
RA   Ron M., Alandete Saez M., Eshed Williams L., Fletcher J.C., McCormick S.;
RT   "Proper regulation of a sperm-specific cis-nat-siRNA is essential for
RT   double fertilization in Arabidopsis.";
RL   Genes Dev. 24:1010-1021(2010).
CC   -!- FUNCTION: Regulates positively reproductive function by facilitating
CC       male gametophyte formation and double fertilization.
CC       {ECO:0000269|PubMed:20478994}.
CC   -!- TISSUE SPECIFICITY: Mostly expressed in pollen and open flowers and, to
CC       a lower extent, in closed flowers. {ECO:0000269|PubMed:20478994}.
CC   -!- DEVELOPMENTAL STAGE: During male gametophyte development, first
CC       observed in bicellular pollen, and accumulates gradually during pollen
CC       maturation in tricellular pollen and mature pollen.
CC       {ECO:0000269|PubMed:20478994}.
CC   -!- DISRUPTION PHENOTYPE: Altered male gametophytic development with
CC       frequent single-fertilization events leading to reduced seed set due to
CC       undeveloped ovules. Increased levels of ARI14 in sperm.
CC       {ECO:0000269|PubMed:20478994}.
CC   -!- MISCELLANEOUS: Transcripts of KPL and ARI14, encoded by adjacent genes
CC       organized in a reverse orientation, have the potential to generate
CC       natural cis-antisense siRNAs (cis-nat-siRNAs) pair targeting ARI14 in
CC       sperm, thus leading to opposite expression levels during male
CC       gametophyte development. {ECO:0000269|PubMed:20478994}.
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DR   EMBL; AB005234; BAB10465.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED97789.1; -; Genomic_DNA.
DR   EMBL; AY924878; AAX23953.1; -; Genomic_DNA.
DR   RefSeq; NP_201177.1; NM_125767.2.
DR   AlphaFoldDB; Q9FFP2; -.
DR   SMR; Q9FFP2; -.
DR   STRING; 3702.AT5G63720.1; -.
DR   PaxDb; Q9FFP2; -.
DR   PRIDE; Q9FFP2; -.
DR   EnsemblPlants; AT5G63720.1; AT5G63720.1; AT5G63720.
DR   GeneID; 836492; -.
DR   Gramene; AT5G63720.1; AT5G63720.1; AT5G63720.
DR   KEGG; ath:AT5G63720; -.
DR   Araport; AT5G63720; -.
DR   TAIR; locus:2160659; AT5G63720.
DR   eggNOG; ENOG502S0F7; Eukaryota.
DR   HOGENOM; CLU_587100_0_0_1; -.
DR   InParanoid; Q9FFP2; -.
DR   OMA; HHESKEQ; -.
DR   OrthoDB; 912276at2759; -.
DR   PRO; PR:Q9FFP2; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FFP2; baseline and differential.
DR   GO; GO:0009567; P:double fertilization forming a zygote and endosperm; IMP:TAIR.
DR   GO; GO:0009555; P:pollen development; IMP:TAIR.
PE   2: Evidence at transcript level;
KW   Developmental protein; Fertilization; Reference proteome.
FT   CHAIN           1..492
FT                   /note="Protein KOKOPELLI"
FT                   /id="PRO_0000439863"
FT   REGION          218..354
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          394..426
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        290..336
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        337..352
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   492 AA;  55950 MW;  D496AEF2726BDE9C CRC64;
     MEPNNVERNL QSLRRLYSLL VANARNEYIP EAYKLDDNTQ FLLKRLLDFA SHEHFVTQSN
     LLATQLRVFP KTVLHGAKPS NVADSSETTP PAMLSQINGS HITRVSKPLE AKGTLQRDLR
     VDQIRSNPSK DVLTEEVVDA IEQIDTQLSA LSFVSSRVDS DERTRSVKSF VTPPTEECRQ
     NSQASMPCLR SNYMTVSQKS VISGAEVTFP YNDQLVRVTS PPQPLPPRAV SGFKKPNQSN
     RASQKMPIMK PTLMDQETET FDDDSSETEA DQTPSATGSE SEDEEVSTSQ EYSGETGSSS
     GSEWETQAEN DTESKSESSY PPQNDDSVSE VSTSPPHTDR DTSREPGKQR RNVMGRFKRI
     KNKIGQIFHH HHHHHHHHHH HDKEKPSAWN KLQSKFHHKH QEKSKERKRP MSESKGLTTH
     KQQHQGGHFH ALVEGLVRHR KHSKKQKHQL KSDAKKTEWW KLLKKRQGGG VKIPKRGRVK
     LGKKKHYLSK NV