ID   KPPR_MESCR              Reviewed;         397 AA.
AC   P27774;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Phosphoribulokinase, chloroplastic;
DE            Short=PRK;
DE            Short=PRKase;
DE            EC=2.7.1.19;
DE   AltName: Full=Phosphopentokinase;
DE   Flags: Precursor;
OS   Mesembryanthemum crystallinum (Common ice plant) (Cryophytum crystallinum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Aizoaceae; Mesembryanthemum;
OC   Mesembryanthemum subgen. Cryophytum.
OX   NCBI_TaxID=3544;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Michalowski C.B., Derocher E.J., Bohnert H.J., Salvucci M.E.;
RT   "Phosphoribulokinase from ice plant: transcription, transcripts and protein
RT   expression during environmental stress.";
RL   Submitted (JUL-1991) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-ribulose 5-phosphate = ADP + D-ribulose 1,5-
CC         bisphosphate + H(+); Xref=Rhea:RHEA:19365, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:57870, ChEBI:CHEBI:58121,
CC         ChEBI:CHEBI:456216; EC=2.7.1.19;
CC   -!- ACTIVITY REGULATION: Light regulated via thioredoxin by reversible
CC       oxidation/reduction of sulfhydryl/disulfide groups.
CC   -!- PATHWAY: Carbohydrate biosynthesis; Calvin cycle.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the phosphoribulokinase family. {ECO:0000305}.
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DR   EMBL; M73707; AAA33034.1; -; mRNA.
DR   PIR; T12436; T12436.
DR   AlphaFoldDB; P27774; -.
DR   SMR; P27774; -.
DR   PRIDE; P27774; -.
DR   UniPathway; UPA00116; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008974; F:phosphoribulokinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0019253; P:reductive pentose-phosphate cycle; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR006082; PRK.
DR   InterPro; IPR006083; PRK/URK.
DR   Pfam; PF00485; PRK; 1.
DR   PRINTS; PR00478; PHRIBLKINASE.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00567; PHOSPHORIBULOKINASE; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Calvin cycle; Chloroplast; Disulfide bond; Kinase;
KW   Nucleotide-binding; Photosynthesis; Plastid; Transferase; Transit peptide.
FT   TRANSIT         1..44
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000250"
FT   CHAIN           45..397
FT                   /note="Phosphoribulokinase, chloroplastic"
FT                   /id="PRO_0000025753"
FT   DISULFID        62..101
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   397 AA;  44114 MW;  88DC418E211EC975 CRC64;
     MAVSAYTVPT TSHLGFNQKK QLFFCNKSAY KRVSFSSRPC VITCLAGDSQ TIVIGLAADS
     GCGKSTFMRR LTSVFGGAAE PPRGGNPDSN TLISDTTTVI CLDDYHSLDR TGRKEKGVTA
     LDPRANDFDL MYEQVKALKE GKAVEKPIYN HVTGLLDAPE LIKPPKILVI EGLHPMFDSR
     VRDLLDFSIY LDISNEVKFA WKIQRDMAER GHSLESIKAS IEARKPDFDA YIDPQKQYAD
     AVIEVLPTQL IPGDNEGKVL RVRLIQKEGV QYFSPVYLFD EGSSITWIPC GRKLTCSYPG
     IKFFYGPDTY FGNEVTVLEM DGQFDRLDEL IYVESHLSNL STKFYGEVTQ QMLKHQDFPG
     SNNGTGLFQT IVGLKIRDLF EQLIASKTAA PAAATKA