ID   KPPR_PINPS              Reviewed;          35 AA.
AC   P81664;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1999, sequence version 1.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=Phosphoribulokinase;
DE            Short=PRK;
DE            Short=PRKase;
DE            EC=2.7.1.19;
DE   AltName: Full=Phosphopentokinase;
DE   Flags: Fragments;
OS   Pinus pinaster (Maritime pine).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Pinus;
OC   Pinus subgen. Pinus.
OX   NCBI_TaxID=71647;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Needle;
RX   PubMed=10344291;
RX   DOI=10.1002/(sici)1522-2683(19990101)20:4/5<1098::aid-elps1098>3.0.co;2-z;
RA   Costa P., Pionneau C., Bauw G., Dubos C., Bahrman N., Kremer A.,
RA   Frigerio J.-M., Plomion C.;
RT   "Separation and characterization of needle and xylem maritime pine
RT   proteins.";
RL   Electrophoresis 20:1098-1108(1999).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-ribulose 5-phosphate = ADP + D-ribulose 1,5-
CC         bisphosphate + H(+); Xref=Rhea:RHEA:19365, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:57870, ChEBI:CHEBI:58121,
CC         ChEBI:CHEBI:456216; EC=2.7.1.19;
CC   -!- ACTIVITY REGULATION: Light regulated via thioredoxin by reversible
CC       oxidation/reduction of sulfhydryl/disulfide groups. {ECO:0000250}.
CC   -!- PATHWAY: Carbohydrate biosynthesis; Calvin cycle.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- MISCELLANEOUS: On the 2D-gel the determined pI of this protein (spot
CC       N148) is: 5.6, its MW is: 37 kDa.
CC   -!- SIMILARITY: Belongs to the phosphoribulokinase family. {ECO:0000305}.
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DR   AlphaFoldDB; P81664; -.
DR   SMR; P81664; -.
DR   PRIDE; P81664; -.
DR   UniPathway; UPA00116; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008974; F:phosphoribulokinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0019253; P:reductive pentose-phosphate cycle; IEA:UniProtKB-UniPathway.
PE   1: Evidence at protein level;
KW   ATP-binding; Calvin cycle; Chloroplast; Direct protein sequencing; Kinase;
KW   Nucleotide-binding; Photosynthesis; Plastid; Transferase.
FT   CHAIN           <1..>35
FT                   /note="Phosphoribulokinase"
FT                   /id="PRO_0000201961"
FT   NON_CONS        12..13
FT                   /evidence="ECO:0000305"
FT   NON_CONS        23..24
FT                   /evidence="ECO:0000305"
FT   NON_TER         1
FT   NON_TER         35
SQ   SEQUENCE   35 AA;  4175 MW;  78679369686ED5E1 CRC64;
     ANNFDLMYEQ VKFYGEVTQQ MLKVSVLEMD GQFDR