位置:首页 > 蛋白库 > ARAP_BACSU
ARAP_BACSU
ID   ARAP_BACSU              Reviewed;         313 AA.
AC   P94529; O05094;
DT   03-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 129.
DE   RecName: Full=Arabinooligosaccharides transport system permease protein AraP {ECO:0000305};
GN   Name=araP {ECO:0000303|PubMed:9084180}; Synonyms=yseD;
GN   OrderedLocusNames=BSU28740;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TRANSCRIPTIONAL REGULATION.
RC   STRAIN=168;
RX   PubMed=9084180; DOI=10.1099/00221287-143-3-957;
RA   Sa-Nogueira I.M.G., Nogueira T.V., Soares S., de Lencastre H.;
RT   "The Bacillus subtilis L-arabinose (ara) operon: nucleotide sequence,
RT   genetic organization and expression.";
RL   Microbiology 143:957-969(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969504; DOI=10.1099/13500872-142-11-3067;
RA   Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J.,
RA   Emmerson P.T., Harwood C.R.;
RT   "The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis
RT   chromosome containing genes responsible for stress responses, the
RT   utilization of plant cell walls and primary metabolism.";
RL   Microbiology 142:3067-3078(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   TRANSCRIPTIONAL REGULATION.
RX   PubMed=10417639; DOI=10.1046/j.1365-2958.1999.01484.x;
RA   Mota L.J., Tavares P., Sa-Nogueira I.M.G.;
RT   "Mode of action of AraR, the key regulator of L-arabinose metabolism in
RT   Bacillus subtilis.";
RL   Mol. Microbiol. 33:476-489(1999).
RN   [5]
RP   FUNCTION, AND SUBUNIT.
RX   PubMed=20693325; DOI=10.1128/jb.00832-10;
RA   Ferreira M.J., Sa-Nogueira I.D.;
RT   "A multitask ATPase serving different ABC-type sugar importers in Bacillus
RT   subtilis.";
RL   J. Bacteriol. 192:5312-5318(2010).
RN   [6]
RP   MUTAGENESIS OF GLU-205; GLU-208 AND ASP-213.
RX   PubMed=29240795; DOI=10.1371/journal.pone.0189483;
RA   Ferreira M.J., Mendes A.L., de Sa-Nogueira I.;
RT   "The MsmX ATPase plays a crucial role in pectin mobilization by Bacillus
RT   subtilis.";
RL   PLoS ONE 12:e0189483-e0189483(2017).
CC   -!- FUNCTION: Part of the ABC transporter complex AraNPQ involved in the
CC       uptake of arabinooligosaccharides. Transports alpha-1,5-
CC       arabinooligosaccharides, at least up to four L-arabinosyl units
CC       (PubMed:20693325). Responsible for the translocation of the substrate
CC       across the membrane (Probable). {ECO:0000269|PubMed:20693325,
CC       ECO:0000305|PubMed:20693325}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (MsmX),
CC       two transmembrane proteins (AraP and AraQ) and a solute-binding protein
CC       (AraN). {ECO:0000269|PubMed:20693325}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Transcription is repressed by glucose and by the binding of
CC       AraR to the operon promoter. L-arabinose acts as an inducer by
CC       inhibiting the binding of AraR to the DNA, thus allowing expression of
CC       the gene. {ECO:0000269|PubMed:10417639, ECO:0000269|PubMed:9084180}.
CC   -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC       permease family. MalFG subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X89810; CAA61935.1; -; Genomic_DNA.
DR   EMBL; Z75208; CAA99593.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14834.1; -; Genomic_DNA.
DR   PIR; B69588; B69588.
DR   RefSeq; NP_390752.1; NC_000964.3.
DR   RefSeq; WP_003229507.1; NZ_JNCM01000036.1.
DR   AlphaFoldDB; P94529; -.
DR   SMR; P94529; -.
DR   STRING; 224308.BSU28740; -.
DR   TCDB; 3.A.1.1.34; the atp-binding cassette (abc) superfamily.
DR   PaxDb; P94529; -.
DR   PRIDE; P94529; -.
DR   EnsemblBacteria; CAB14834; CAB14834; BSU_28740.
DR   GeneID; 937432; -.
DR   KEGG; bsu:BSU28740; -.
DR   PATRIC; fig|224308.179.peg.3122; -.
DR   eggNOG; COG1175; Bacteria.
DR   InParanoid; P94529; -.
DR   OMA; CSFPFVM; -.
DR   PhylomeDB; P94529; -.
DR   BioCyc; BSUB:BSU28740-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   CDD; cd06261; TM_PBP2; 1.
DR   Gene3D; 1.10.3720.10; -; 1.
DR   InterPro; IPR000515; MetI-like.
DR   InterPro; IPR035906; MetI-like_sf.
DR   Pfam; PF00528; BPD_transp_1; 1.
DR   SUPFAM; SSF161098; SSF161098; 1.
DR   PROSITE; PS50928; ABC_TM1; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Reference proteome; Sugar transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..313
FT                   /note="Arabinooligosaccharides transport system permease
FT                   protein AraP"
FT                   /id="PRO_0000059954"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        126..146
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        176..196
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        224..244
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        281..301
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   DOMAIN          87..302
FT                   /note="ABC transmembrane type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   MUTAGEN         205
FT                   /note="E->A: Has no effect on the growth rate."
FT                   /evidence="ECO:0000269|PubMed:29240795"
FT   MUTAGEN         208
FT                   /note="E->A: Small negative impact in the uptake of
FT                   arabinotriose. Shows an increase of about 10% in the
FT                   doubling time."
FT                   /evidence="ECO:0000269|PubMed:29240795"
FT   MUTAGEN         213
FT                   /note="D->A: Severely impairs the uptake of arabinotriose.
FT                   Shows a 3.6-fold increase of the doubling time."
FT                   /evidence="ECO:0000269|PubMed:29240795"
FT   CONFLICT        186
FT                   /note="R -> K (in Ref. 1; CAA61935)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   313 AA;  35035 MW;  F140CF2D7566F5A7 CRC64;
     MKPVKTGTVH PVPSAAKQSG WRDLFYSKKA APYLFTAPFV LSFLVFFLYP IISVFIMSFQ
     RILPGEVSFV GLSNYTALNN PTFYTALWNT LEYTFWTLIV LIPVPLLLAI FLNSKLVKFR
     NIFKSALFIP ALTSTIVAGI IFRLIFGEME TSLANSILLK LGFSPQNWMN NEHTGMFLMV
     LLASWRWMGI NILYFLAGLQ NVPKELYEAA DIDGANTMKK FLHITLPFLK PVTVYVLTIS
     IIGGFRMFEE SYVLWQNNSP GNIGLTLVGY LYQQGLAYNE MGYGAAIGIV LLIVILVVSL
     ISLKLSGSFK GEG
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024