ARAR_BACSU
ID ARAR_BACSU Reviewed; 362 AA.
AC P96711;
DT 03-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2002, sequence version 3.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Arabinose metabolism transcriptional repressor;
GN Name=araR; Synonyms=araC, yvbS; OrderedLocusNames=BSU33970;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9045819; DOI=10.1128/jb.179.5.1598-1608.1997;
RA Sa-Nogueira I.M.G., Mota L.J.;
RT "Negative regulation of L-arabinose metabolism in Bacillus subtilis:
RT characterization of the araR (araC) gene.";
RL J. Bacteriol. 179:1598-1608(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP PROTEIN SEQUENCE OF 1-10, AND CHARACTERIZATION.
RX PubMed=10417639; DOI=10.1046/j.1365-2958.1999.01484.x;
RA Mota L.J., Tavares P., Sa-Nogueira I.M.G.;
RT "Mode of action of AraR, the key regulator of L-arabinose metabolism in
RT Bacillus subtilis.";
RL Mol. Microbiol. 33:476-489(1999).
RN [4]
RP CHARACTERIZATION.
RC STRAIN=168;
RX PubMed=11418559; DOI=10.1128/jb.183.14.4190-4201.2001;
RA Mota L.J., Sarmento L.M., Sa-Nogueira I.M.G.;
RT "Control of the arabinose regulon in Bacillus subtilis by AraR in vivo:
RT crucial roles of operators, cooperativity, and DNA looping.";
RL J. Bacteriol. 183:4190-4201(2001).
CC -!- FUNCTION: Transcriptional repressor of the arabinose utilization genes.
CC Also regulates its own expression. Binds to two sequences within the
CC promoters of the araABDLMNPQ-abfA operon and the araE gene, and to one
CC sequence in the araR promoter.
CC -!- ACTIVITY REGULATION: Binding to DNA is inhibited by L-arabinose.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
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DR EMBL; X98354; CAA66999.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15402.2; -; Genomic_DNA.
DR PIR; D69588; D69588.
DR RefSeq; NP_391277.1; NC_000964.3.
DR RefSeq; WP_003243070.1; NZ_JNCM01000033.1.
DR PDB; 3TB6; X-ray; 2.21 A; A/B=71-362.
DR PDB; 4EGY; X-ray; 2.30 A; A/B=1-68.
DR PDB; 4EGZ; X-ray; 2.30 A; A/B=1-68.
DR PDB; 4H0E; X-ray; 1.97 A; A/B=1-68.
DR PDB; 5D4R; X-ray; 2.07 A; A/B=1-68.
DR PDB; 5D4S; X-ray; 1.97 A; A/B=1-68.
DR PDBsum; 3TB6; -.
DR PDBsum; 4EGY; -.
DR PDBsum; 4EGZ; -.
DR PDBsum; 4H0E; -.
DR PDBsum; 5D4R; -.
DR PDBsum; 5D4S; -.
DR AlphaFoldDB; P96711; -.
DR SMR; P96711; -.
DR STRING; 224308.BSU33970; -.
DR PaxDb; P96711; -.
DR PRIDE; P96711; -.
DR EnsemblBacteria; CAB15402; CAB15402; BSU_33970.
DR GeneID; 938635; -.
DR KEGG; bsu:BSU33970; -.
DR PATRIC; fig|224308.179.peg.3683; -.
DR eggNOG; COG1609; Bacteria.
DR InParanoid; P96711; -.
DR OMA; DYIFPRI; -.
DR BioCyc; BSUB:BSU33970-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR CDD; cd01541; PBP1_AraR; 1.
DR CDD; cd07377; WHTH_GntR; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR033532; AraR_ligand_bind_dom.
DR InterPro; IPR046335; LacI/GalR-like_sensor.
DR InterPro; IPR028082; Peripla_BP_I.
DR InterPro; IPR000524; Tscrpt_reg_HTH_GntR.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00392; GntR; 1.
DR Pfam; PF13377; Peripla_BP_3; 1.
DR PRINTS; PR00035; HTHGNTR.
DR SMART; SM00345; HTH_GNTR; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF53822; SSF53822; 1.
DR PROSITE; PS50949; HTH_GNTR; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Direct protein sequencing; DNA-binding;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..362
FT /note="Arabinose metabolism transcriptional repressor"
FT /id="PRO_0000050620"
FT DOMAIN 1..70
FT /note="HTH gntR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00307"
FT DNA_BIND 30..49
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00307"
FT HELIX 4..15
FT /evidence="ECO:0007829|PDB:5D4S"
FT TURN 16..19
FT /evidence="ECO:0007829|PDB:5D4S"
FT HELIX 30..37
FT /evidence="ECO:0007829|PDB:5D4S"
FT HELIX 41..53
FT /evidence="ECO:0007829|PDB:5D4S"
FT STRAND 56..60
FT /evidence="ECO:0007829|PDB:5D4S"
FT TURN 61..63
FT /evidence="ECO:0007829|PDB:5D4S"
FT STRAND 64..67
FT /evidence="ECO:0007829|PDB:5D4S"
FT STRAND 81..87
FT /evidence="ECO:0007829|PDB:3TB6"
FT STRAND 89..91
FT /evidence="ECO:0007829|PDB:3TB6"
FT HELIX 94..107
FT /evidence="ECO:0007829|PDB:3TB6"
FT STRAND 111..116
FT /evidence="ECO:0007829|PDB:3TB6"
FT HELIX 121..133
FT /evidence="ECO:0007829|PDB:3TB6"
FT STRAND 137..141
FT /evidence="ECO:0007829|PDB:3TB6"
FT HELIX 153..161
FT /evidence="ECO:0007829|PDB:3TB6"
FT STRAND 166..171
FT /evidence="ECO:0007829|PDB:3TB6"
FT STRAND 180..183
FT /evidence="ECO:0007829|PDB:3TB6"
FT HELIX 185..198
FT /evidence="ECO:0007829|PDB:3TB6"
FT STRAND 203..212
FT /evidence="ECO:0007829|PDB:3TB6"
FT HELIX 213..228
FT /evidence="ECO:0007829|PDB:3TB6"
FT HELIX 235..237
FT /evidence="ECO:0007829|PDB:3TB6"
FT STRAND 238..241
FT /evidence="ECO:0007829|PDB:3TB6"
FT HELIX 243..246
FT /evidence="ECO:0007829|PDB:3TB6"
FT HELIX 249..260
FT /evidence="ECO:0007829|PDB:3TB6"
FT TURN 261..263
FT /evidence="ECO:0007829|PDB:3TB6"
FT STRAND 267..270
FT /evidence="ECO:0007829|PDB:3TB6"
FT HELIX 274..286
FT /evidence="ECO:0007829|PDB:3TB6"
FT TURN 291..294
FT /evidence="ECO:0007829|PDB:3TB6"
FT STRAND 296..298
FT /evidence="ECO:0007829|PDB:3TB6"
FT HELIX 305..308
FT /evidence="ECO:0007829|PDB:3TB6"
FT STRAND 309..311
FT /evidence="ECO:0007829|PDB:3TB6"
FT STRAND 314..317
FT /evidence="ECO:0007829|PDB:3TB6"
FT HELIX 321..335
FT /evidence="ECO:0007829|PDB:3TB6"
FT STRAND 345..347
FT /evidence="ECO:0007829|PDB:3TB6"
FT STRAND 350..352
FT /evidence="ECO:0007829|PDB:3TB6"
SQ SEQUENCE 362 AA; 40488 MW; 679DC8FEE69FB421 CRC64;
MLPKYAQVKE EISSWINQGK ILPDQKIPTE NELMQQFGVS RHTIRKAIGD LVSQGLLYSV
QGGGTFVASR SAKSALHSNK TIGVLTTYIS DYIFPSIIRG IESYLSEQGY SMLLTSTNNN
PDNERRGLEN LLSQHIDGLI VEPTKSALQT PNIGYYLNLE KNGIPFAMIN ASYAELAAPS
FTLDDVKGGM MAAEHLLSLG HTHMMGIFKA DDTQGVKRMN GFIQAHRERE LFPSPDMIVT
FTTEEKESKL LEKVKATLEK NSKHMPTAIL CYNDEIALKV IDMLREMDLK VPEDMSIVGY
DDSHFAQISE VKLTSVKHPK SVLGKAAAKY VIDCLEHKKP KQEDVIFEPE LIIRQSARKL
NE
