ID   ARB1_SCHPO              Reviewed;         399 AA.
AC   Q9P7B8;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Argonaute-binding protein 1;
GN   Name=arb1; ORFNames=SPAC140.03;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1] {ECO:0000312|EMBL:CAB86414.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2] {ECO:0000305}
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
RN   [3] {ECO:0000305}
RP   FUNCTION, INTERACTION WITH AGO1, IDENTIFICATION IN THE ARC COMPLEX, AND
RP   SUBCELLULAR LOCATION.
RX   PubMed=17310250; DOI=10.1038/nsmb1211;
RA   Buker S.M., Iida T., Buehler M., Villen J., Gygi S.P., Nakayama J.,
RA   Moazed D.;
RT   "Two different Argonaute complexes are required for siRNA generation and
RT   heterochromatin assembly in fission yeast.";
RL   Nat. Struct. Mol. Biol. 14:200-207(2007).
CC   -!- FUNCTION: Component of the argonaute siRNA chaperone (ARC) complex
CC       which is required for histone H3K9 methylation, heterochromatin
CC       assembly and siRNA generation. The ARC complex contains mostly double-
CC       stranded siRNA. Inhibits the release of the siRNA passenger strand from
CC       ago1 together with arb2. Inhibits the slicer activity of ago1. Required
CC       for swi6 localization to the centromeric repeats.
CC       {ECO:0000269|PubMed:17310250}.
CC   -!- SUBUNIT: Component of the argonaute siRNA chaperone (ARC) complex
CC       composed of ago1, arb1 and arb2. Interacts with ago1.
CC       {ECO:0000269|PubMed:17310250}.
CC   -!- INTERACTION:
CC       Q9P7B8; O74957: ago1; NbExp=5; IntAct=EBI-15624131, EBI-422882;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16823372,
CC       ECO:0000269|PubMed:17310250}. Cytoplasm {ECO:0000269|PubMed:16823372,
CC       ECO:0000269|PubMed:17310250}. Note=Predominantly nuclear.
CC       {ECO:0000269|PubMed:16823372, ECO:0000269|PubMed:17310250}.
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DR   EMBL; CU329670; CAB86414.1; -; Genomic_DNA.
DR   RefSeq; NP_593532.1; NM_001018966.2.
DR   AlphaFoldDB; Q9P7B8; -.
DR   BioGRID; 279308; 41.
DR   DIP; DIP-29299N; -.
DR   IntAct; Q9P7B8; 2.
DR   STRING; 4896.SPAC140.03.1; -.
DR   MaxQB; Q9P7B8; -.
DR   PaxDb; Q9P7B8; -.
DR   EnsemblFungi; SPAC140.03.1; SPAC140.03.1:pep; SPAC140.03.
DR   GeneID; 2542862; -.
DR   KEGG; spo:SPAC140.03; -.
DR   PomBase; SPAC140.03; arb1.
DR   VEuPathDB; FungiDB:SPAC140.03; -.
DR   eggNOG; ENOG502S0TP; Eukaryota.
DR   HOGENOM; CLU_607149_0_0_1; -.
DR   InParanoid; Q9P7B8; -.
DR   OMA; VRMERFI; -.
DR   PhylomeDB; Q9P7B8; -.
DR   PRO; PR:Q9P7B8; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0033167; C:ARC complex; IDA:PomBase.
DR   GO; GO:0005737; C:cytoplasm; IDA:PomBase.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; IDA:PomBase.
DR   GO; GO:0008428; F:ribonuclease inhibitor activity; IDA:PomBase.
DR   GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR   GO; GO:0031048; P:heterochromatin assembly by small RNA; IMP:PomBase.
DR   InterPro; IPR018606; Arb1.
DR   Pfam; PF09692; Arb1; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Nucleus; Reference proteome; RNA-mediated gene silencing.
FT   CHAIN           1..399
FT                   /note="Argonaute-binding protein 1"
FT                   /id="PRO_0000284724"
SQ   SEQUENCE   399 AA;  45631 MW;  6508689C97C5F1D5 CRC64;
     MAEGDFKNDS TGCIGDSVEF TTFKKRTGKS LGNRRKKRGL SGWEPYFQEP ELTAEEKLEN
     ETLYSRNIPL TVRMERFIQR YRSRRKWSDP TRIRLFTMYL DLGGVSTGQK QFTGGTDINN
     DAKAREVSAQ TTTDYIEYDI LDEYDIDFKW VAGVFLSSHI LYNAGLTKEE DLQIACQIVR
     NFLLSVLHNN VAPEFEDNIR DACLLAEQAE TELISNKRLS TKLPGRLQRA LASIHVDNYK
     GLWDKSQSDR TSDSSVNFIE SNNTKFMPND ELFEPDGISR FSTQQARDYI QRTLGPRYLT
     GKVVEQEYLT VKLVSKTLLN FSNQSLCKAV FIVWDPPGSK YSQDTNKERL EVILESDLLN
     NTVDGTHLEG SFTFLDNGLT ILDTVLAVLP TFYEEVKDE