ID   ARB2_SCHPO              Reviewed;         321 AA.
AC   Q10271;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   22-FEB-2012, sequence version 3.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Argonaute-binding protein 2;
GN   Name=arb2; ORFNames=SPAC13G7.07;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   REVISION OF GENE MODEL.
RX   PubMed=21511999; DOI=10.1126/science.1203357;
RA   Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA   Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA   Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA   Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA   French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA   Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA   Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA   Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA   Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA   Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT   "Comparative functional genomics of the fission yeasts.";
RL   Science 332:930-936(2011).
RN   [3]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
RN   [4]
RP   FUNCTION, COMPOSITION OF THE ARC COMPLEX, AND SUBCELLULAR LOCATION.
RX   PubMed=17310250; DOI=10.1038/nsmb1211;
RA   Buker S.M., Iida T., Buehler M., Villen J., Gygi S.P., Nakayama J.,
RA   Moazed D.;
RT   "Two different Argonaute complexes are required for siRNA generation and
RT   heterochromatin assembly in fission yeast.";
RL   Nat. Struct. Mol. Biol. 14:200-207(2007).
CC   -!- FUNCTION: A member of the argonaute siRNA chaperone (ARC) complex which
CC       is required for histone H3K9 methylation, heterochromatin assembly and
CC       siRNA generation. The ARC complex contains mostly double-stranded
CC       siRNA. Inhibits the release of the siRNA passenger strand from ago1
CC       together with arb1. {ECO:0000269|PubMed:17310250}.
CC   -!- SUBUNIT: Component of the argonaute siRNA chaperone (ARC) complex
CC       composed of ago1, arb1 and arb2.
CC   -!- INTERACTION:
CC       Q10271; O74957: ago1; NbExp=4; IntAct=EBI-15624152, EBI-422882;
CC   -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Localizes to a large
CC       number of foci in both the nucleus and cytoplasm.
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DR   EMBL; CU329670; CAA93595.4; -; Genomic_DNA.
DR   PIR; T37657; T37657.
DR   RefSeq; NP_593709.3; NM_001019140.3.
DR   AlphaFoldDB; Q10271; -.
DR   BioGRID; 279324; 20.
DR   DIP; DIP-29300N; -.
DR   IntAct; Q10271; 2.
DR   STRING; 4896.SPAC13G7.07.1; -.
DR   PaxDb; Q10271; -.
DR   EnsemblFungi; SPAC13G7.07.1; SPAC13G7.07.1:pep; SPAC13G7.07.
DR   GeneID; 2542879; -.
DR   KEGG; spo:SPAC13G7.07; -.
DR   PomBase; SPAC13G7.07; arb2.
DR   VEuPathDB; FungiDB:SPAC13G7.07; -.
DR   HOGENOM; CLU_1034984_0_0_1; -.
DR   InParanoid; Q10271; -.
DR   OMA; NEHYYQA; -.
DR   PRO; PR:Q10271; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0033167; C:ARC complex; IDA:PomBase.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR   GO; GO:0031048; P:heterochromatin assembly by small RNA; IMP:PomBase.
PE   1: Evidence at protein level;
KW   Cytoplasm; Nucleus; Reference proteome; RNA-mediated gene silencing.
FT   CHAIN           1..321
FT                   /note="Argonaute-binding protein 2"
FT                   /id="PRO_0000116575"
SQ   SEQUENCE   321 AA;  37169 MW;  C675802B4B945297 CRC64;
     MFRKKKSRVL QRDVNFGNCL EDMNLQITND FYLRKLNAPN ELYEFQVTKD KEYNEKFYFA
     VLDHVYSWTL QNSLLTRISI PLESSPSEPH SWIYATRNWQ TNENGIVIII QSMSHPLIWS
     NRNLREGDVR RATVLDVIAK CTEKKLSVVI FSPSALLWDQ SINFPRTTRY FTSQGLQITK
     KSIIKDVETP ESYVSIGLNY VMSNSVSTKI HFVGSEYGSY LLNSYLDLNW KKFSGKAGAI
     LLIMNMSINE ELHDPDYVSY LIEHARNYLP SHEPCRHLMN SIMTESGIPN FSAGKYIDEF
     YDYIGDVLLE MIALDTNTSK S