ID   ARBH_ASFM2              Reviewed;         179 AA.
AC   Q07819;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Apoptosis regulator Bcl-2 homolog;
GN   OrderedLocusNames=Mal-049; ORFNames=LMW5-HL;
OS   African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10500;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=85517; Phacochoerus aethiopicus (Warthog).
OH   NCBI_TaxID=41426; Phacochoerus africanus (Warthog).
OH   NCBI_TaxID=273792; Potamochoerus larvatus (Bushpig).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RX   PubMed=8389936; DOI=10.1128/jvi.67.7.4391-4394.1993;
RA   Neilan J.G., Lu Z., Afonso C.L., Kutish G.F., Sussman M.D., Rock D.L.;
RT   "An African swine fever virus gene with similarity to the proto-oncogene
RT   bcl-2 and the Epstein-Barr virus gene BHRF1.";
RL   J. Virol. 67:4391-4394(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Kutish G.F., Rock D.L.;
RT   "African swine fever virus genomes.";
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Suppresses apoptosis in host cell to promote the viral
CC       replication (By similarity). Has the ability to potentially bind to all
CC       the members of the proapoptotic Bcl-2 family (By similarity). Inhibits
CC       autophagy by interacting with host Beclin 1 (BECN1) (By similarity).
CC       {ECO:0000250|UniProtKB:P42485}.
CC   -!- SUBUNIT: Interacts with host BECN1 (via BH3 homology domain); this
CC       interaction allows the virus to inhibit BECN1, and thus autophagy (By
CC       similarity). Interacts with host BID (By similarity). Interacts with
CC       host BAX (By similarity). {ECO:0000250|UniProtKB:P42485}.
CC   -!- SUBCELLULAR LOCATION: Host mitochondrion
CC       {ECO:0000250|UniProtKB:P42485}. Host endoplasmic reticulum
CC       {ECO:0000250|UniProtKB:P42485}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle
CC       (PubMed:8389936). Expressed in the late phase of the viral replicative
CC       cycle (PubMed:8389936). {ECO:0000269|PubMed:8389936}.
CC   -!- SIMILARITY: Belongs to the Bcl-2 family. {ECO:0000305}.
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DR   EMBL; L09548; AAA17034.1; -; Unassigned_DNA.
DR   EMBL; AY261361; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   SMR; Q07819; -.
DR   Proteomes; UP000000860; Genome.
DR   GO; GO:0044165; C:host cell endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR   GO; GO:0033650; C:host cell mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0019050; P:suppression by virus of host apoptotic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.437.10; -; 1.
DR   InterPro; IPR036834; Bcl-2-like_sf.
DR   InterPro; IPR046371; Bcl-2_BH1-3.
DR   InterPro; IPR026298; Bcl-2_fam.
DR   InterPro; IPR002475; Bcl2-like.
DR   InterPro; IPR020717; Bcl2_BH1_motif_CS.
DR   InterPro; IPR020726; Bcl2_BH2_motif_CS.
DR   PANTHER; PTHR11256; PTHR11256; 1.
DR   Pfam; PF00452; Bcl-2; 1.
DR   PRINTS; PR01862; BCL2FAMILY.
DR   SMART; SM00337; BCL; 1.
DR   SUPFAM; SSF56854; SSF56854; 1.
DR   PROSITE; PS50062; BCL2_FAMILY; 1.
DR   PROSITE; PS01080; BH1; 1.
DR   PROSITE; PS01258; BH2; 1.
PE   2: Evidence at transcript level;
KW   Apoptosis; Early protein; Host endoplasmic reticulum; Host mitochondrion;
KW   Host-virus interaction;
KW   Inhibition of host apoptosis by viral BCL2-like protein; Late protein;
KW   Modulation of host cell apoptosis by virus.
FT   CHAIN           1..179
FT                   /note="Apoptosis regulator Bcl-2 homolog"
FT                   /id="PRO_0000002813"
FT   MOTIF           76..95
FT                   /note="BH1"
FT                   /evidence="ECO:0000255"
FT   MOTIF           126..141
FT                   /note="BH2"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   179 AA;  21068 MW;  0A4204D5643C66E4 CRC64;
     MEGEELIYHN IINEILVGYI KYYMNDISEH ELSPYQQQIK KILTYYDECL NKQVTITFSL
     TNAQEIKTQF TGVVTELFKD LINWGRICGF IVFSARMAKY CKDANNHLES TVITTAYNFM
     KHNLLPWMIS HGGQEEFLAF SLHSDIYSVI FNIKYFLSKF CNHMFLRSCV QLLRNCNLI