ARBH_HHV8P
ID ARBH_HHV8P Reviewed; 175 AA.
AC F5HGJ3;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 37.
DE RecName: Full=Apoptosis regulator Bcl-2 homolog;
GN Name=vBCL2; ORFNames=ORF16;
OS Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's
OS sarcoma-associated herpesvirus).
OC Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC Herpesvirales; Herpesviridae; Gammaherpesvirinae; Rhadinovirus.
OX NCBI_TaxID=868565;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=10400794; DOI=10.1128/jvi.73.8.6953-6963.1999;
RA Glenn M., Rainbow L., Aurade F., Davison A., Schulz T.F.;
RT "Identification of a spliced gene from Kaposi's sarcoma-associated
RT herpesvirus encoding a protein with similarities to latent membrane
RT proteins 1 and 2A of Epstein-Barr virus.";
RL J. Virol. 73:6953-6963(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16760382; DOI=10.1099/vir.0.81919-0;
RA Rezaee S.A.R., Cunningham C., Davison A.J., Blackbourn D.J.;
RT "Kaposi's sarcoma-associated herpesvirus immune modulation: an overview.";
RL J. Gen. Virol. 87:1781-1804(2006).
RN [3]
RP FUNCTION.
RX PubMed=9012846; DOI=10.1073/pnas.94.2.690;
RA Cheng E.H., Nicholas J., Bellows D.S., Hayward G.S., Guo H.G., Reitz M.S.,
RA Hardwick J.M.;
RT "A Bcl-2 homolog encoded by Kaposi sarcoma-associated virus, human
RT herpesvirus 8, inhibits apoptosis but does not heterodimerize with Bax or
RT Bak.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:690-694(1997).
RN [4]
RP INTERACTION WITH HUMAN NOP53, SUBCELLULAR LOCATION, AND REGION.
RX PubMed=20042497; DOI=10.1128/jvi.00757-09;
RA Kalt I., Borodianskiy-Shteinberg T., Schachor A., Sarid R.;
RT "GLTSCR2/PICT-1, a putative tumor suppressor gene product, induces the
RT nucleolar targeting of the Kaposi's sarcoma-associated herpesvirus KS-Bcl-2
RT protein.";
RL J. Virol. 84:2935-2945(2010).
RN [5]
RP FUNCTION, AND MUTAGENESIS OF GLU-14.
RX PubMed=25740994; DOI=10.1128/jvi.00102-15;
RA Liang Q., Chang B., Lee P., Brulois K.F., Ge J., Shi M., Rodgers M.A.,
RA Feng P., Oh B.H., Liang C., Jung J.U.;
RT "Identification of the Essential Role of Viral Bcl-2 for Kaposi's Sarcoma-
RT Associated Herpesvirus Lytic Replication.";
RL J. Virol. 89:5308-5317(2015).
RN [6]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=28053098; DOI=10.1128/jvi.01875-16;
RA Gallo A., Lampe M., Guenther T., Brune W.;
RT "The viral Bcl-2 homologs of Kaposi's sarcoma-associated herpesvirus and
RT rhesus rhadinovirus share an essential role for viral replication.";
RL J. Virol. 91:0-0(2017).
RN [7]
RP FUNCTION, AND INTERACTION WITH ORF55.
RX PubMed=29167347; DOI=10.1128/jvi.00914-17;
RA Liang Q., Wei D., Chung B., Brulois K.F., Guo C., Dong S., Gao S.J.,
RA Feng P., Liang C., Jung J.U.;
RT "Novel Role of vBcl2 in the Virion Assembly of Kaposi's Sarcoma-Associated
RT Herpesvirus.";
RL J. Virol. 92:0-0(2018).
CC -!- FUNCTION: Plays a role in the protection against apoptosis mediated by
CC cytotoxic cells during the immune response to acute and persistent
CC viral infection. Contributes therefore to latency establishment. Plays
CC also a role in the inhibition of host starvation-induced autophagy
CC which ultimately contributes to the viral chronic infection.
CC Participates also in the viral genome replication within host nucleus
CC (PubMed:28053098). {ECO:0000269|PubMed:25740994,
CC ECO:0000269|PubMed:28053098, ECO:0000269|PubMed:29167347,
CC ECO:0000269|PubMed:9012846}.
CC -!- SUBUNIT: Interacts with human NOP53; may sequester ORF16 in host
CC nucleolus and reduce its antiapoptotic activity. Interacts with ORF55.
CC {ECO:0000269|PubMed:20042497, ECO:0000269|PubMed:29167347}.
CC -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}. Host mitochondrion {ECO:0000269|PubMed:20042497,
CC ECO:0000269|PubMed:28053098}. Host nucleus, host nucleolus
CC {ECO:0000269|PubMed:20042497}. Host nucleus
CC {ECO:0000269|PubMed:28053098}.
CC -!- SIMILARITY: Belongs to the Bcl-2 family. {ECO:0000305}.
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DR EMBL; AF148805; ABD28866.1; -; Genomic_DNA.
DR RefSeq; YP_001129368.1; NC_009333.1.
DR SMR; F5HGJ3; -.
DR BioGRID; 1776950; 8.
DR PRIDE; F5HGJ3; -.
DR DNASU; 4961447; -.
DR GeneID; 4961447; -.
DR KEGG; vg:4961447; -.
DR Proteomes; UP000000942; Genome.
DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0033650; C:host cell mitochondrion; IDA:UniProtKB.
DR GO; GO:0044196; C:host cell nucleolus; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0019050; P:suppression by virus of host apoptotic process; IMP:UniProtKB.
DR Gene3D; 1.10.437.10; -; 1.
DR InterPro; IPR036834; Bcl-2-like_sf.
DR InterPro; IPR046371; Bcl-2_BH1-3.
DR InterPro; IPR002475; Bcl2-like.
DR Pfam; PF00452; Bcl-2; 1.
DR SMART; SM00337; BCL; 1.
DR SUPFAM; SSF56854; SSF56854; 1.
DR PROSITE; PS50062; BCL2_FAMILY; 1.
PE 1: Evidence at protein level;
KW Host membrane; Host mitochondrion; Host nucleus; Host-virus interaction;
KW Inhibition of host apoptosis by viral BCL2-like protein; Membrane;
KW Modulation of host cell apoptosis by virus; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..175
FT /note="Apoptosis regulator Bcl-2 homolog"
FT /id="PRO_0000423772"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 37..42
FT /note="Mediates interaction with human NOP53 and
FT localization to host nucleolus"
FT /evidence="ECO:0000269|PubMed:20042497"
FT MUTAGEN 14
FT /note="E->A: Complete loss of viral lytic replication."
FT /evidence="ECO:0000269|PubMed:25740994"
SQ SEQUENCE 175 AA; 19404 MW; 5A13A3DB1902C306 CRC64;
MDEDVLPGEV LAIEGIFMAC GLNEPEYLYH PLLSPIKLYI TGLMRDKESL FEAMLANVRF
HSTTGINQLG LSMLQVSGDG NMNWGRALAI LTFGSFVAQK LSNEPHLRDF ALAVLPVYAY
EAIGPQWFRA RGGWRGLKAY CTQVLTRRRG RRMTALLGSI ALLATILAAV AMSRR
