ARBK1_MOUSE
ID ARBK1_MOUSE Reviewed; 689 AA.
AC Q99MK8; Q99LL8;
DT 24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2004, sequence version 2.
DT 03-AUG-2022, entry version 178.
DE RecName: Full=Beta-adrenergic receptor kinase 1;
DE Short=Beta-ARK-1;
DE EC=2.7.11.15 {ECO:0000250|UniProtKB:P26817};
DE AltName: Full=G-protein-coupled receptor kinase 2 {ECO:0000312|MGI:MGI:87940};
GN Name=Grk2 {ECO:0000312|MGI:MGI:87940}; Synonyms=Adrbk1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Lymphoma;
RX PubMed=8638670; DOI=10.1152/ajpcell.1996.270.3.c885;
RA Hughes R.J., Anderson K.L., Kiel D., Insel P.A.;
RT "Cloning of GRK2 cDNA from S49 murine lymphoma cells.";
RL Am. J. Physiol. 270:C885-C891(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-689.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=16452087; DOI=10.1074/mcp.t500041-mcp200;
RA Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.;
RT "Comprehensive identification of phosphorylation sites in postsynaptic
RT density preparations.";
RL Mol. Cell. Proteomics 5:914-922(2006).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-670, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Lung, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP FUNCTION.
RX PubMed=30284927; DOI=10.1152/ajplung.00135.2018;
RA Yim P.D., Gallos G., Perez-Zoghbi J.F., Zhang Y., Xu D., Wu A.,
RA Berkowitz D.E., Emala C.W.;
RT "Airway smooth muscle photorelaxation via opsin receptor activation.";
RL Am. J. Physiol. 316:L82-L93(2019).
CC -!- FUNCTION: Specifically phosphorylates the agonist-occupied form of the
CC beta-adrenergic and closely related receptors, probably inducing a
CC desensitization of them (By similarity). Key regulator of LPAR1
CC signaling (By similarity). Competes with RALA for binding to LPAR1 thus
CC affecting the signaling properties of the receptor (By similarity).
CC Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner
CC (By similarity). Positively regulates ciliary smoothened (SMO)-
CC dependent Hedgehog (Hh) signaling pathway by facilitating the
CC trafficking of SMO into the cilium and the stimulation of SMO activity
CC (By similarity). Inhibits relaxation of airway smooth muscle in
CC response to blue light (PubMed:30284927).
CC {ECO:0000250|UniProtKB:P21146, ECO:0000250|UniProtKB:P25098,
CC ECO:0000269|PubMed:30284927}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[beta-adrenergic receptor] + ATP = [beta-adrenergic receptor]-
CC phosphate + ADP + H(+); Xref=Rhea:RHEA:19429, Rhea:RHEA-COMP:11222,
CC Rhea:RHEA-COMP:11223, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43176, ChEBI:CHEBI:68546, ChEBI:CHEBI:456216;
CC EC=2.7.11.15; Evidence={ECO:0000250|UniProtKB:P26817};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19430;
CC Evidence={ECO:0000250|UniProtKB:P26817};
CC -!- ACTIVITY REGULATION: In contrast to other AGC family kinases, the
CC catalytic activity is solely regulated by the binding of substrates and
CC ligands, not by phosphorylation of the kinase domain.
CC {ECO:0000250|UniProtKB:P21146}.
CC -!- SUBUNIT: Interacts with the heterodimer formed by GNB1 and GNG2 (By
CC similarity). Interacts with GIT1 (By similarity). Interacts with, and
CC phosphorylates chemokine-stimulated CCR5 (By similarity). Interacts
CC with ARRB1 (By similarity). Interacts with LPAR1 and LPAR2. Interacts
CC with RALA in response to LPAR1 activation (By similarity). ADRBK1 and
CC RALA mutually inhibit each other's binding to LPAR1 (By similarity).
CC Interacts with ADRB2 (By similarity). {ECO:0000250|UniProtKB:P21146,
CC ECO:0000250|UniProtKB:P25098, ECO:0000250|UniProtKB:P26817}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P26817}. Cell
CC membrane {ECO:0000250|UniProtKB:P21146}. Postsynapse
CC {ECO:0000250|UniProtKB:P26817}. Presynapse
CC {ECO:0000250|UniProtKB:P26817}.
CC -!- DOMAIN: The PH domain binds anionic phospholipids and helps recruiting
CC ADRBK1 from the cytoplasm to plasma membrane close to activated
CC receptors. It mediates binding to G protein beta and gamma subunits,
CC competing with G-alpha subunits and other G-betagamma effectors.
CC {ECO:0000250|UniProtKB:P21146}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr
CC protein kinase family. GPRK subfamily. {ECO:0000305}.
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DR EMBL; AF333028; AAK21896.1; -; mRNA.
DR EMBL; BC003196; AAH03196.1; -; mRNA.
DR CCDS; CCDS70915.1; -.
DR RefSeq; NP_001277747.1; NM_001290818.1.
DR AlphaFoldDB; Q99MK8; -.
DR SMR; Q99MK8; -.
DR BioGRID; 225525; 15.
DR DIP; DIP-32413N; -.
DR ELM; Q99MK8; -.
DR IntAct; Q99MK8; 3.
DR STRING; 10090.ENSMUSP00000025791; -.
DR ChEMBL; CHEMBL3711486; -.
DR iPTMnet; Q99MK8; -.
DR PhosphoSitePlus; Q99MK8; -.
DR EPD; Q99MK8; -.
DR jPOST; Q99MK8; -.
DR MaxQB; Q99MK8; -.
DR PaxDb; Q99MK8; -.
DR PRIDE; Q99MK8; -.
DR ProteomicsDB; 273917; -.
DR Antibodypedia; 16429; 728 antibodies from 42 providers.
DR DNASU; 110355; -.
DR Ensembl; ENSMUST00000088737; ENSMUSP00000086114; ENSMUSG00000024858.
DR GeneID; 110355; -.
DR KEGG; mmu:110355; -.
DR UCSC; uc008fzu.2; mouse.
DR CTD; 156; -.
DR MGI; MGI:87940; Grk2.
DR VEuPathDB; HostDB:ENSMUSG00000024858; -.
DR eggNOG; KOG0986; Eukaryota.
DR GeneTree; ENSGT00940000161626; -.
DR InParanoid; Q99MK8; -.
DR OMA; FIQRSPN; -.
DR OrthoDB; 1104340at2759; -.
DR PhylomeDB; Q99MK8; -.
DR BRENDA; 2.7.11.16; 3474.
DR Reactome; R-MMU-111933; Calmodulin induced events.
DR Reactome; R-MMU-416476; G alpha (q) signalling events.
DR Reactome; R-MMU-418555; G alpha (s) signalling events.
DR Reactome; R-MMU-5635838; Activation of SMO.
DR Reactome; R-MMU-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR BioGRID-ORCS; 110355; 5 hits in 80 CRISPR screens.
DR ChiTaRS; Grk2; mouse.
DR PRO; PR:Q99MK8; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q99MK8; protein.
DR Bgee; ENSMUSG00000024858; Expressed in granulocyte and 284 other tissues.
DR ExpressionAtlas; Q99MK8; baseline and differential.
DR Genevisible; Q99MK8; MM.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0016324; C:apical plasma membrane; ISO:MGI.
DR GO; GO:0030424; C:axon; ISO:MGI.
DR GO; GO:0016323; C:basolateral plasma membrane; ISO:MGI.
DR GO; GO:0005901; C:caveola; ISO:MGI.
DR GO; GO:0005929; C:cilium; TAS:Reactome.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0043198; C:dendritic shaft; ISO:MGI.
DR GO; GO:0043197; C:dendritic spine; ISO:MGI.
DR GO; GO:0016020; C:membrane; IDA:MGI.
DR GO; GO:0045121; C:membrane raft; ISO:MGI.
DR GO; GO:0005739; C:mitochondrion; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IEA:GOC.
DR GO; GO:0098793; C:presynapse; IEA:UniProtKB-SubCell.
DR GO; GO:0045202; C:synapse; ISO:MGI.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0047696; F:beta-adrenergic receptor kinase activity; ISO:MGI.
DR GO; GO:0031850; F:delta-type opioid receptor binding; ISO:MGI.
DR GO; GO:0031755; F:Edg-2 lysophosphatidic acid receptor binding; ISO:MGI.
DR GO; GO:0001664; F:G protein-coupled receptor binding; ISO:MGI.
DR GO; GO:0004703; F:G protein-coupled receptor kinase activity; IDA:MGI.
DR GO; GO:0031851; F:kappa-type opioid receptor binding; ISO:MGI.
DR GO; GO:0004672; F:protein kinase activity; IDA:MGI.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IDA:MGI.
DR GO; GO:0097110; F:scaffold protein binding; ISO:MGI.
DR GO; GO:0031625; F:ubiquitin protein ligase binding; ISO:MGI.
DR GO; GO:0060048; P:cardiac muscle contraction; ISO:MGI.
DR GO; GO:1990869; P:cellular response to chemokine; ISO:MGI.
DR GO; GO:0071364; P:cellular response to epidermal growth factor stimulus; ISO:MGI.
DR GO; GO:0071333; P:cellular response to glucose stimulus; ISO:MGI.
DR GO; GO:0002029; P:desensitization of G protein-coupled receptor signaling pathway; IMP:MGI.
DR GO; GO:0042699; P:follicle-stimulating hormone signaling pathway; ISO:MGI.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IDA:MGI.
DR GO; GO:0007507; P:heart development; IMP:MGI.
DR GO; GO:0051457; P:maintenance of protein location in nucleus; ISO:MGI.
DR GO; GO:0106072; P:negative regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway; ISO:MGI.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISO:MGI.
DR GO; GO:1900077; P:negative regulation of cellular response to insulin stimulus; ISO:MGI.
DR GO; GO:2000117; P:negative regulation of cysteine-type endopeptidase activity; ISO:MGI.
DR GO; GO:0045744; P:negative regulation of G protein-coupled receptor signaling pathway; ISO:MGI.
DR GO; GO:0046325; P:negative regulation of glucose import; ISO:MGI.
DR GO; GO:0033137; P:negative regulation of peptidyl-serine phosphorylation; ISO:MGI.
DR GO; GO:0010801; P:negative regulation of peptidyl-threonine phosphorylation; ISO:MGI.
DR GO; GO:1901081; P:negative regulation of relaxation of smooth muscle; IMP:UniProtKB.
DR GO; GO:0045988; P:negative regulation of striated muscle contraction; ISO:MGI.
DR GO; GO:0003108; P:negative regulation of the force of heart contraction by chemical signal; ISO:MGI.
DR GO; GO:0048709; P:oligodendrocyte differentiation; ISO:MGI.
DR GO; GO:0018105; P:peptidyl-serine phosphorylation; IDA:MGI.
DR GO; GO:0018107; P:peptidyl-threonine phosphorylation; IDA:MGI.
DR GO; GO:0033605; P:positive regulation of catecholamine secretion; ISO:MGI.
DR GO; GO:2000463; P:positive regulation of excitatory postsynaptic potential; ISO:MGI.
DR GO; GO:0048146; P:positive regulation of fibroblast proliferation; ISO:MGI.
DR GO; GO:0032755; P:positive regulation of interleukin-6 production; ISO:MGI.
DR GO; GO:0010661; P:positive regulation of muscle cell apoptotic process; ISO:MGI.
DR GO; GO:1904058; P:positive regulation of sensory perception of pain; ISO:MGI.
DR GO; GO:1904707; P:positive regulation of vascular associated smooth muscle cell proliferation; ISO:MGI.
DR GO; GO:0045907; P:positive regulation of vasoconstriction; ISO:MGI.
DR GO; GO:0006468; P:protein phosphorylation; IDA:MGI.
DR GO; GO:0031623; P:receptor internalization; ISO:MGI.
DR GO; GO:0009966; P:regulation of signal transduction; IBA:GO_Central.
DR GO; GO:0002026; P:regulation of the force of heart contraction; IGI:MGI.
DR GO; GO:0014070; P:response to organic cyclic compound; ISO:MGI.
DR GO; GO:0006979; P:response to oxidative stress; ISO:MGI.
DR GO; GO:0007217; P:tachykinin receptor signaling pathway; ISO:MGI.
DR GO; GO:0042311; P:vasodilation; ISO:MGI.
DR GO; GO:0046718; P:viral entry into host cell; ISO:MGI.
DR GO; GO:0019079; P:viral genome replication; ISO:MGI.
DR Gene3D; 1.10.167.10; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR000961; AGC-kinase_C.
DR InterPro; IPR000239; GPCR_kinase.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR016137; RGS.
DR InterPro; IPR036305; RGS_sf.
DR InterPro; IPR044926; RGS_subdomain_2.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF00069; Pkinase; 1.
DR Pfam; PF00615; RGS; 1.
DR PRINTS; PR00717; GPCRKINASE.
DR SMART; SM00233; PH; 1.
DR SMART; SM00315; RGS; 1.
DR SMART; SM00133; S_TK_X; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF48097; SSF48097; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS51285; AGC_KINASE_CTER; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
DR PROSITE; PS50132; RGS; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cell membrane; Cell projection; Cytoplasm; Kinase; Membrane;
KW Nucleotide-binding; Phosphoprotein; Reference proteome;
KW Serine/threonine-protein kinase; Synapse; Transferase.
FT CHAIN 1..689
FT /note="Beta-adrenergic receptor kinase 1"
FT /id="PRO_0000085629"
FT DOMAIN 54..175
FT /note="RGS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00171"
FT DOMAIN 191..453
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT DOMAIN 454..521
FT /note="AGC-kinase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00618"
FT DOMAIN 558..652
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 1..190
FT /note="N-terminal"
FT ACT_SITE 317
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 197..205
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 220
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT SITE 3
FT /note="Required for receptor phosphorylation"
FT /evidence="ECO:0000250|UniProtKB:P25098"
FT SITE 4
FT /note="Required for receptor phosphorylation"
FT /evidence="ECO:0000250|UniProtKB:P25098"
FT SITE 10
FT /note="Required for receptor phosphorylation"
FT /evidence="ECO:0000250|UniProtKB:P25098"
FT MOD_RES 670
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 160
FT /note="D -> N (in Ref. 1; AAK21896)"
FT /evidence="ECO:0000305"
FT CONFLICT 374
FT /note="W -> C (in Ref. 1; AAK21896)"
FT /evidence="ECO:0000305"
FT CONFLICT 520
FT /note="E -> V (in Ref. 1; AAK21896)"
FT /evidence="ECO:0000305"
FT CONFLICT 600
FT /note="L -> F (in Ref. 1; AAK21896)"
FT /evidence="ECO:0000305"
FT CONFLICT 679
FT /note="P -> R (in Ref. 1; AAK21896)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 689 AA; 79639 MW; 508F32B287DD99C6 CRC64;
MADLEAVLAD VSYLMAMEKS KATPAARASK KILLPEPSIR SVMQKYLEDR GEVTFEKIFS
QKLGYLLFRD FCLNHLEEAK PLVEFYEEIK KYEKLETEEE RVVRSREIFD SYIMKELLAC
SHPFSKNATE HVQGHLVKKQ VPPDLFQPYI EEICQNLRGD VFQKFIESDK FTRFCQWKNV
ELNIHLTMND FSVHRIIGRG GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER
IMLSLVSTGD CPFIVCMSYA FHTPDKLSFI LDLMNGGDLH YHLSQHGVFS EADMRFYAAE
IILGLEHMHN RFVVYRDLKP ANILLDEHGH VRISDLGLAC DFSKKRPHAS VGTHGYMAPE
VLQKGVAYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH EIDRMTLTMA VELPDSFSPE
LRSLLEGLLQ RDVNRRLGCL GRGAQEVKES PFFRSLDWQM VFLQKYPPPL IPPRGEVNAA
DAFDIGSFDE EDTKGIKLLD SDQELYRNFP LTISERWQQE VAETVFDTIN AETDRLEARK
KAKNKQLGHE EDYALGKDCI VHGYMSKMGN PFLTQWQRRY FYLFPNRLEW RGEGEAPQSL
LTMEEIQSVE ETQIKERKCL LLKIRGGKQF VLQCDSDPEL VQWKKELRDA YREAQQLVQR
VPKMKNKPRS PVVELSKVPL IQRGSANGL
