KRAK_DROME
ID KRAK_DROME Reviewed; 331 AA.
AC O18391;
DT 15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Probable serine hydrolase;
DE EC=3.1.-.-;
DE AltName: Full=Kraken protein;
GN Name=kraken; ORFNames=CG3943;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Embryo;
RX PubMed=9831651; DOI=10.1016/s0378-1119(98)00497-1;
RA Chan H.Y.E., Harris S.J., O'Kane C.J.;
RT "Identification and characterization of kraken, a gene encoding a putative
RT hydrolytic enzyme in Drosophila melanogaster.";
RL Gene 222:195-201(1998).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: May have a role in detoxification and digestion during
CC embryogenesis and larval development.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed before embryonic stage 11.
CC At stage 11, expression is concentrated in the foregut and posterior
CC midgut. By stage 15, in gastric caeca, pharynx, posterior spiracles and
CC anterior edge of midgut. At the end of embryogenesis, expression is
CC confined to gastric caeca. During third instar larvae, expressed at low
CC levels in gastric caeca, midgut and hindgut and high level in fat body.
CC -!- DEVELOPMENTAL STAGE: Probably expressed both maternally and
CC zygotically.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. {ECO:0000305}.
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DR EMBL; AJ000516; CAA04153.1; -; Genomic_DNA.
DR EMBL; AE014134; AAF51445.1; -; Genomic_DNA.
DR EMBL; AY095092; AAM11420.1; -; mRNA.
DR EMBL; AY118303; AAM48332.1; -; mRNA.
DR RefSeq; NP_001259847.1; NM_001272918.1.
DR RefSeq; NP_477265.1; NM_057917.4.
DR AlphaFoldDB; O18391; -.
DR SMR; O18391; -.
DR ESTHER; drome-KRAKEN; SERHL.
DR MEROPS; S33.A81; -.
DR PaxDb; O18391; -.
DR PRIDE; O18391; -.
DR DNASU; 33265; -.
DR EnsemblMetazoa; FBtr0078016; FBpp0077681; FBgn0020545.
DR EnsemblMetazoa; FBtr0334794; FBpp0306841; FBgn0020545.
DR GeneID; 33265; -.
DR KEGG; dme:Dmel_CG3943; -.
DR UCSC; CG3943-RA; d. melanogaster.
DR CTD; 33265; -.
DR FlyBase; FBgn0020545; kraken.
DR VEuPathDB; VectorBase:FBgn0020545; -.
DR eggNOG; KOG1454; Eukaryota.
DR GeneTree; ENSGT00510000047225; -.
DR HOGENOM; CLU_020336_8_1_1; -.
DR InParanoid; O18391; -.
DR OMA; RGLMPVP; -.
DR OrthoDB; 1285824at2759; -.
DR PhylomeDB; O18391; -.
DR BioGRID-ORCS; 33265; 0 hits in 3 CRISPR screens.
DR ChiTaRS; kraken; fly.
DR GenomeRNAi; 33265; -.
DR PRO; PR:O18391; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0020545; Expressed in adult Malpighian tubule (Drosophila) and 50 other tissues.
DR ExpressionAtlas; O18391; baseline and differential.
DR Genevisible; O18391; DM.
DR GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IBA:GO_Central.
DR GO; GO:0017171; F:serine hydrolase activity; HDA:FlyBase.
DR GO; GO:0007586; P:digestion; IEP:UniProtKB.
DR GO; GO:0009636; P:response to toxic substance; IEP:UniProtKB.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR Pfam; PF00561; Abhydrolase_1; 1.
DR PRINTS; PR00111; ABHYDROLASE.
DR SUPFAM; SSF53474; SSF53474; 1.
DR PROSITE; PS00120; LIPASE_SER; 1.
PE 2: Evidence at transcript level;
KW Detoxification; Developmental protein; Digestion; Hydrolase;
KW Reference proteome; Serine esterase.
FT CHAIN 1..331
FT /note="Probable serine hydrolase"
FT /id="PRO_0000084323"
FT DOMAIN 63..163
FT /note="AB hydrolase-1"
FT /evidence="ECO:0000255"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 138
FT /evidence="ECO:0000250"
SQ SEQUENCE 331 AA; 37094 MW; 1FE39BC42AED4E69 CRC64;
MGQTRVAATT AAQSPAAELS PETNGQTEEP LQLLGEDSWE EFSIAVPWGT VEAKWWGSKE
RQPIIALHGW QDNCGSFDRL CPLLPADTSI LAIDLPGHGK SSHYPMGMQY FIFWDGICLI
RRIVRKYNWK NVTLLGHSLG GALTFMYAAS FPTEVEKLIN IDIAGPTVRG TQRMAEGTGR
ALDKFLDYET LPESKQPCYS YDEMIKLVLD AYDGSVDEPS VRVLMNRGMR HNPSKNGYLF
ARDLRLKVSL LGMFTAEQTL AYARQIRCRV LNIRGIPGMK FETPQVYADV IATLRENAAK
VVYVEVPGTH HLHLVTPDRV APHIIRFLKE A
