ID   KRB2_VACCA              Reviewed;         283 AA.
AC   O57259;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Pseudokinase B12;
GN   Name=B12; OrderedLocusNames=MVA180R, ACAM3000_MVA_180;
OS   Vaccinia virus (strain Ankara) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=126794;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=9601507; DOI=10.1006/viro.1998.9123;
RA   Antoine G., Scheiflinger F., Dorner F., Falkner F.G.;
RT   "The complete genomic sequence of the modified vaccinia Ankara strain:
RT   comparison with other orthopoxviruses.";
RL   Virology 244:365-396(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Isolate Acambis 3000;
RA   Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J.,
RA   Khristova M., Wohlhueter R.M.;
RL   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Pseudokinase that plays a role in viral DNA replication
CC       repression by activating the antiviral protein BANF1 and inhibiting the
CC       activity of host VRK1, a cellular modulator of BANF1.
CC       {ECO:0000250|UniProtKB:P24362}.
CC   -!- ACTIVITY REGULATION: Both catalytically active kinases B1/VPK1 and host
CC       VRK2 repress B12 inhibitory activity in a B1/VPK1 deletion mutant
CC       strain. {ECO:0000250|UniProtKB:P24362}.
CC   -!- SUBUNIT: Interacts with B1/VPK1. Interacts with host VRK1. Interacts
CC       with host VRK2. {ECO:0000250|UniProtKB:P24362}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000250|UniProtKB:P24362}.
CC   -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC       kinase family. Poxviruses subfamily. {ECO:0000255|PROSITE-
CC       ProRule:PRU00159}.
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DR   EMBL; U94848; AAB96553.1; -; Genomic_DNA.
DR   EMBL; AY603355; AAT10576.1; -; Genomic_DNA.
DR   PIR; T37448; T37448.
DR   SMR; O57259; -.
DR   Proteomes; UP000159908; Genome.
DR   Proteomes; UP000172909; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR000719; Prot_kinase_dom.
DR   InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR   Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Host nucleus; Kinase; Nucleotide-binding;
KW   Serine/threonine-protein kinase; Transferase.
FT   CHAIN           1..283
FT                   /note="Pseudokinase B12"
FT                   /id="PRO_0000086195"
FT   DOMAIN          1..283
FT                   /note="Protein kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
SQ   SEQUENCE   283 AA;  33338 MW;  BCE8B01DF40A3B6D CRC64;
     MESFKYCFDN DGKKWIIGNT LYSGNSILYK VRKNFTSSFY NYVMKIDHKS HKPLLSEIRF
     YISVLDPLTI DNWTRERGIK YLAIPDLYGI GETDDYMFFV IKNLGRVFAP KDTESVFEAC
     VTMINTLEFI HSRGFTHGKI EPRNILIRNK RLSLIDYSRT NKLYKSGNSH IDYNEDMITS
     GNINYMCVDN HLGATVSKRG DLEMLGYCMI EWFGGKLPWK NESSIKVIKQ KKEYKKFIAT
     FFEDCFPEGN EPLELVRYIE LVYTLDYSQT PNYDRLRRLF IQD