KRBBA_MOUSE
ID KRBBA_MOUSE Reviewed; 223 AA.
AC P27812; A2RSI6; Q1WJB7; Q548H2; Q61969;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Killer cell lectin-like receptor subfamily B member 1B allele A;
DE AltName: Full=CD161 antigen-like family member B;
DE AltName: Full=Lymphocyte antigen 55b;
DE Short=Ly-55b;
DE AltName: Full=NKR-P1 34;
DE AltName: Full=Natural killer cell surface protein NKR-P1B allele SJL/BALB;
DE Short=NKR-P1B;
DE AltName: CD_antigen=CD161b;
GN Name=Klrb1b; Synonyms=Ly55b, Nkrp1b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J; TISSUE=Natural killer cell;
RX PubMed=1880421;
RA Giorda R., Trucco M.;
RT "Mouse NKR-P1. A family of genes selectively coexpressed in adherent
RT lymphokine-activated killer cells.";
RL J. Immunol. 147:1701-1708(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=SJL/J;
RX PubMed=10229823;
RA Kung S.K.P., Su R.-C., Shannon J., Miller R.G.;
RT "The NKR-P1B gene product is an inhibitory receptor on SJL/J NK cells.";
RL J. Immunol. 162:5876-5887(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANTS VAL-183 AND THR-191.
RC STRAIN=BALB/cJ; TISSUE=Spleen;
RX PubMed=16751398; DOI=10.4049/jimmunol.176.12.7511;
RA Carlyle J.R., Mesci A., Ljutic B., Belanger S., Tai L.-H., Rousselle E.,
RA Troke A.D., Proteau M.-F., Makrigiannis A.P.;
RT "Molecular and genetic basis for strain-dependent NK1.1 alloreactivity of
RT mouse NK cells.";
RL J. Immunol. 176:7511-7524(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-29, AND NUCLEOTIDE SEQUENCE [GENOMIC DNA]
RP OF 10-198.
RC STRAIN=BALB/cJ;
RX PubMed=1517565;
RA Giorda R., Weisberg E.P., Ip T.K., Trucco M.;
RT "Genomic structure and strain-specific expression of the natural killer
RT cell receptor NKR-P1.";
RL J. Immunol. 149:1957-1963(1992).
RN [6]
RP FUNCTION, AND INTERACTION WITH PTPN6.
RX PubMed=10229828;
RA Carlyle J.R., Martin A., Mehra A., Attisano L., Tsui F.W.,
RA Zuniga-Pfluecker J.C.;
RT "Mouse NKR-P1B, a novel NK1.1 antigen with inhibitory function.";
RL J. Immunol. 162:5917-5923(1999).
RN [7]
RP FUNCTION, AND INTERACTION WITH CLEC2D.
RX PubMed=14990792; DOI=10.1073/pnas.0308304101;
RA Carlyle J.R., Jamieson A.M., Gasser S., Clingan C.S., Arase H.,
RA Raulet D.H.;
RT "Missing self-recognition of Ocil/Clr-b by inhibitory NKR-P1 natural killer
RT cell receptors.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:3527-3532(2004).
RN [8]
RP INTERACTION WITH LCK AND PTPN6, DOMAIN ITIM MOTIF AND LCK-BINDING MOTIF,
RP AND MUTAGENESIS OF LEU-6; TYR-8 AND CYS-32.
RX PubMed=15814704; DOI=10.4049/jimmunol.174.8.4789;
RA Ljutic B., Carlyle J.R., Filipp D., Nakagawa R., Julius M.,
RA Zuniga-Pfluecker J.C.;
RT "Functional requirements for signaling through the stimulatory and
RT inhibitory mouse NKR-P1 (CD161) NK cell receptors.";
RL J. Immunol. 174:4789-4796(2005).
CC -!- FUNCTION: Receptor for CLEC2D/OCIL. Ligand-binding contributes to
CC inhibition of cytotoxic natural killer (NK) cells. May mediate MHC
CC class I-independent 'missing-self' recognition of allografts, tumor
CC cells and virus-infected cells. {ECO:0000269|PubMed:10229823,
CC ECO:0000269|PubMed:10229828, ECO:0000269|PubMed:14990792}.
CC -!- SUBUNIT: Homodimer; disulfide-linked. Interacts with tyrosine kinase
CC LCK. Binds PTPN6/SHP-1 in a phosphorylation-dependent manner.
CC {ECO:0000269|PubMed:10229828, ECO:0000269|PubMed:14990792,
CC ECO:0000269|PubMed:15814704}.
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed in NK cells and a subset of T-cells.
CC -!- DOMAIN: Contains 1 copy of a cytoplasmic motif that is referred to as
CC the immunoreceptor tyrosine-based inhibitor motif (ITIM). The
CC phosphorylated ITIM motif can bind the SH2 domain of several SH2-
CC containing phosphatases leading to down-regulation of cell activation.
CC {ECO:0000269|PubMed:15814704}.
CC -!- POLYMORPHISM: Variants Thr-191 and Ala-191 interfere with binding of
CC the anti-NK1.1 monoclonal antibody PK136 which identifies NK cells from
CC C57BL/6 and SJL but not BALB/c mice by binding Klrb1b and Klrb1c in an
CC allele-dependent manner. Mutagenesis of Thr-191 to Ser-191 restores
CC NK1.1 reactivity to Klrb1b from BALB/c mice.
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DR EMBL; M77677; AAA39823.1; -; mRNA.
DR EMBL; AF354259; AAK39099.1; -; mRNA.
DR EMBL; DQ237928; ABB72026.1; -; mRNA.
DR EMBL; BC132126; AAI32127.1; -; mRNA.
DR EMBL; BC132128; AAI32129.1; -; mRNA.
DR EMBL; X64719; CAA45972.1; -; mRNA.
DR EMBL; X64721; CAA45974.1; -; Genomic_DNA.
DR PIR; B46467; B46467.
DR AlphaFoldDB; P27812; -.
DR SMR; P27812; -.
DR PhosphoSitePlus; P27812; -.
DR MaxQB; P27812; -.
DR PRIDE; P27812; -.
DR ProteomicsDB; 265025; -.
DR UCSC; uc009eeq.2; mouse.
DR MGI; MGI:107539; Klrb1b.
DR InParanoid; P27812; -.
DR PhylomeDB; P27812; -.
DR ChiTaRS; Klrb1b; mouse.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; P27812; protein.
DR GO; GO:0009986; C:cell surface; IBA:GO_Central.
DR GO; GO:0009897; C:external side of plasma membrane; IDA:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:MGI.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR GO; GO:0042802; F:identical protein binding; IPI:MGI.
DR GO; GO:0038023; F:signaling receptor activity; IDA:MGI.
DR GO; GO:0045953; P:negative regulation of natural killer cell mediated cytotoxicity; IDA:MGI.
DR GO; GO:0042269; P:regulation of natural killer cell mediated cytotoxicity; IBA:GO_Central.
DR CDD; cd03593; CLECT_NK_receptors_like; 1.
DR Gene3D; 3.10.100.10; -; 1.
DR InterPro; IPR001304; C-type_lectin-like.
DR InterPro; IPR016186; C-type_lectin-like/link_sf.
DR InterPro; IPR016187; CTDL_fold.
DR InterPro; IPR033992; NKR-like_CTLD.
DR Pfam; PF00059; Lectin_C; 1.
DR SMART; SM00034; CLECT; 1.
DR SUPFAM; SSF56436; SSF56436; 1.
DR PROSITE; PS50041; C_TYPE_LECTIN_2; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Lectin; Membrane; Receptor; Reference proteome;
KW Signal-anchor; Transmembrane; Transmembrane helix.
FT CHAIN 1..223
FT /note="Killer cell lectin-like receptor subfamily B member
FT 1B allele A"
FT /id="PRO_0000046676"
FT TOPO_DOM 1..43
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..63
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 64..223
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT DOMAIN 93..212
FT /note="C-type lectin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT MOTIF 6..11
FT /note="ITIM motif"
FT /evidence="ECO:0000269|PubMed:15814704"
FT MOTIF 32..35
FT /note="LCK-binding motif"
FT /evidence="ECO:0000269|PubMed:15814704"
FT DISULFID 122..210
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 189..202
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT VARIANT 183
FT /note="D -> V (in strain: BALB/c)"
FT /evidence="ECO:0000269|PubMed:16751398"
FT VARIANT 191
FT /note="S -> T (in strain: BALB/c; loss of NK1.1
FT reactivity)"
FT /evidence="ECO:0000269|PubMed:16751398"
FT MUTAGEN 6
FT /note="L->S: Loss of inhibition of Ca(2+) influx upon
FT activation of natural killer cells."
FT /evidence="ECO:0000269|PubMed:15814704"
FT MUTAGEN 8
FT /note="Y->F: Loss of PTPN6 binding and inhibition of Ca(2+)
FT influx upon activation of natural killer cells."
FT /evidence="ECO:0000269|PubMed:15814704"
FT MUTAGEN 32
FT /note="C->S: Loss of Klrb1b-mediated inhibition of natural
FT killer cell cytotoxicity. Reduced LCK binding, loss of
FT PTPN6 binding and loss of inhibition of Ca(2+) influx upon
FT activation of natural killer cells."
FT /evidence="ECO:0000269|PubMed:15814704"
FT CONFLICT 217
FT /note="E -> K (in Ref. 3; ABB72026)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 223 AA; 25158 MW; 8D04C11DEBAB9C56 CRC64;
MDSTTLVYAD LNLARIQEPK HDSPPSLSPD TCRCPRWHRL ALKFGCAGLI LLVLVVIGLC
VLVLSVQKSS VQKICADVQE NRTHTTDCSV NLECPQDWLS HRDKCFRVFQ VSNTWEEGQA
DCGRKGATLL LIQDQEELRF LLDSIKEKYN SFWIGLRFTL PDMNWKWING TTFNSDVLKI
TGDTENGSCA SISGDKVTSE SCSTDNRWIC QKELNHETPS NDS
