ID   KRE28_ASHGO             Reviewed;         361 AA.
AC   Q751U7;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 2.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Spindle pole body component KRE28;
GN   Name=KRE28; OrderedLocusNames=AFR728W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Required for kinetochore binding by a discrete subset of
CC       kMAPs and motors. Involved in kinetochore-microtubule binding and the
CC       spindle assembly checkpoint (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC       center, spindle pole body {ECO:0000250}. Nucleus membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Nucleoplasmic
CC       side {ECO:0000250}. Chromosome, centromere, kinetochore {ECO:0000250}.
CC       Note=Localizes to the nuclear side of the spindle pole body.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the KRE28 family. {ECO:0000305}.
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DR   EMBL; AE016819; AAS54100.2; -; Genomic_DNA.
DR   RefSeq; NP_986276.2; NM_212412.2.
DR   AlphaFoldDB; Q751U7; -.
DR   SMR; Q751U7; -.
DR   STRING; 33169.AAS54100; -.
DR   EnsemblFungi; AAS54100; AAS54100; AGOS_AFR728W.
DR   GeneID; 4622567; -.
DR   KEGG; ago:AGOS_AFR728W; -.
DR   eggNOG; ENOG502S19U; Eukaryota.
DR   HOGENOM; CLU_062083_0_0_1; -.
DR   InParanoid; Q751U7; -.
DR   OMA; LADECHT; -.
DR   Proteomes; UP000000591; Chromosome VI.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR   GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005816; C:spindle pole body; IEA:UniProtKB-SubCell.
DR   InterPro; IPR031361; Kre28.
DR   Pfam; PF17097; Kre28; 1.
PE   3: Inferred from homology;
KW   Centromere; Chromosome; Coiled coil; Cytoplasm; Cytoskeleton; Kinetochore;
KW   Membrane; Nucleus; Reference proteome.
FT   CHAIN           1..361
FT                   /note="Spindle pole body component KRE28"
FT                   /id="PRO_0000408560"
FT   COILED          126..160
FT                   /evidence="ECO:0000255"
FT   COILED          220..246
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   361 AA;  41228 MW;  B4A4CA5F755EFDF8 CRC64;
     MSTKDTSGQS GYANDIRKLG EQTAHVSEQV LVQQERQRLG ALEELHQSII QIAEENSFVT
     PIKKDAANVH IDPRGIAVSV QQFKQLAEVL KVTHLEQETL DNFLRYTISD NDQLLDIKSV
     ADSRYARLAE EVCQLEQEEL RHLENEIISL NGNITEQTTK VIDANEKVKE ECLEVSNGIE
     RCWGLLNELE TLRSTTDEGN VELGPLEETY QKWKSVDHFL QQKLHLKEQL RVLEDTRKSL
     SEVTKSSGNR APDLDASEKF VTYRLLDSMW KKQFVDTTKI RDLELYPRTG KIQFQVADTI
     YVLAISGDQI SNIQLFNDKL PAADLENNTQ DLNKRFLGTS DVRRVVDFIT HQQAVPQAQV
     H