ID   ARC2B_XENLA             Reviewed;         300 AA.
AC   Q6IRB1;
DT   10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 53.
DE   RecName: Full=Actin-related protein 2/3 complex subunit 2-B;
GN   Name=arpc2-b; ORFNames=XELAEV_18046980mg {ECO:0000312|EMBL:OCT60954.1};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=J;
RX   PubMed=27762356; DOI=10.1038/nature19840;
RA   Session A.M., Uno Y., Kwon T., Chapman J.A., Toyoda A., Takahashi S.,
RA   Fukui A., Hikosaka A., Suzuki A., Kondo M., van Heeringen S.J., Quigley I.,
RA   Heinz S., Ogino H., Ochi H., Hellsten U., Lyons J.B., Simakov O.,
RA   Putnam N., Stites J., Kuroki Y., Tanaka T., Michiue T., Watanabe M.,
RA   Bogdanovic O., Lister R., Georgiou G., Paranjpe S.S., van Kruijsbergen I.,
RA   Shu S., Carlson J., Kinoshita T., Ohta Y., Mawaribuchi S., Jenkins J.,
RA   Grimwood J., Schmutz J., Mitros T., Mozaffari S.V., Suzuki Y., Haramoto Y.,
RA   Yamamoto T.S., Takagi C., Heald R., Miller K., Haudenschild C., Kitzman J.,
RA   Nakayama T., Izutsu Y., Robert J., Fortriede J., Burns K., Lotay V.,
RA   Karimi K., Yasuoka Y., Dichmann D.S., Flajnik M.F., Houston D.W.,
RA   Shendure J., DuPasquier L., Vize P.D., Zorn A.M., Ito M., Marcotte E.M.,
RA   Wallingford J.B., Ito Y., Asashima M., Ueno N., Matsuda Y., Veenstra G.J.,
RA   Fujiyama A., Harland R.M., Taira M., Rokhsar D.S.;
RT   "Genome evolution in the allotetraploid frog Xenopus laevis.";
RL   Nature 538:336-343(2016).
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE ARP2/3 COMPLEX, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=29925947; DOI=10.1038/s41586-018-0237-5;
RA   Schrank B.R., Aparicio T., Li Y., Chang W., Chait B.T., Gundersen G.G.,
RA   Gottesman M.E., Gautier J.;
RT   "Nuclear ARP2/3 drives DNA break clustering for homology-directed repair.";
RL   Nature 559:61-66(2018).
CC   -!- FUNCTION: Actin-binding component of the Arp2/3 complex, a multiprotein
CC       complex that mediates actin polymerization upon stimulation by
CC       nucleation-promoting factor (NPF). The Arp2/3 complex mediates the
CC       formation of branched actin networks in the cytoplasm, providing the
CC       force for cell motility (By similarity). In addition to its role in the
CC       cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin
CC       polymerization in the nucleus, thereby regulating gene transcription
CC       and repair of damaged DNA (Probable). The Arp2/3 complex promotes
CC       homologous recombination (HR) repair in response to DNA damage by
CC       promoting nuclear actin polymerization, leading to drive motility of
CC       double-strand breaks (DSBs) (By similarity).
CC       {ECO:0000250|UniProtKB:O15144, ECO:0000250|UniProtKB:Q0IH88,
CC       ECO:0000305|PubMed:29925947}.
CC   -!- SUBUNIT: Component of the Arp2/3 complex composed of actr2/arp2,
CC       actr3/arp3, arpc1 (arpc1a or arpc1b), arpc2, arpc3, arpc4 and arpc5.
CC       {ECO:0000269|PubMed:29925947}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000250|UniProtKB:Q0IH88}. Cell projection
CC       {ECO:0000250|UniProtKB:Q0IH88}. Nucleus {ECO:0000269|PubMed:29925947}.
CC   -!- SIMILARITY: Belongs to the ARPC2 family. {ECO:0000305}.
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DR   EMBL; BC070989; AAH70989.1; -; mRNA.
DR   EMBL; CM004483; OCT60954.1; -; Genomic_DNA.
DR   RefSeq; NP_001085042.1; NM_001091573.1.
DR   AlphaFoldDB; Q6IRB1; -.
DR   SMR; Q6IRB1; -.
DR   STRING; 8355.Q6IRB1; -.
DR   DNASU; 432109; -.
DR   GeneID; 432109; -.
DR   KEGG; xla:432109; -.
DR   CTD; 432109; -.
DR   OMA; FPRHVES; -.
DR   OrthoDB; 1345377at2759; -.
DR   Proteomes; UP000186698; Chromosome 9_10S.
DR   Bgee; 432109; Expressed in spleen and 19 other tissues.
DR   GO; GO:0005885; C:Arp2/3 protein complex; IDA:UniProtKB.
DR   GO; GO:0042995; C:cell projection; IEA:UniProtKB-SubCell.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0035861; C:site of double-strand break; IDA:UniProtKB.
DR   GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR   GO; GO:0030041; P:actin filament polymerization; IEA:InterPro.
DR   GO; GO:0034314; P:Arp2/3 complex-mediated actin nucleation; IEA:InterPro.
DR   GO; GO:0030833; P:regulation of actin filament polymerization; IEA:InterPro.
DR   Gene3D; 3.30.1460.20; -; 2.
DR   InterPro; IPR007188; ARPC2.
DR   InterPro; IPR034666; ARPC2/4.
DR   PANTHER; PTHR12058; PTHR12058; 1.
DR   Pfam; PF04045; P34-Arc; 1.
DR   SUPFAM; SSF69645; SSF69645; 2.
PE   1: Evidence at protein level;
KW   Actin-binding; Cell projection; Cytoplasm; Cytoskeleton; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..300
FT                   /note="Actin-related protein 2/3 complex subunit 2-B"
FT                   /id="PRO_0000445560"
SQ   SEQUENCE   300 AA;  34339 MW;  84E127295D437E90 CRC64;
     MILLEVNNRI IEEILTLKFE NAAAGNKPEV VEVTFADFDG VLYHVSNPNG DKAKVMISIS
     LKFYKELQEH GADEVLKNVY GNFLVAPESG YNVSLLYDLE ALPSNKDSVI HQAGMLKRNC
     FASVFEKYFK FQEEGKEGEK RAVIHYREDE TMYVEAKKDR VTVVFSTVFK DDDDVVIGKV
     FMQEFKEGRR ASHTAPQVLF SHREPPLELK DTDAAVGDNI GYITFVLFPR HTSANARDNT
     INLIHTFRDY LHYHIKCSKA YIHTRMRAKT SDFLKVLNRA RPDAEKKEMK TITGKTFAAR