ID   KRE28_YEAS2             Reviewed;         385 AA.
AC   C7GKW3;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   13-OCT-2009, sequence version 1.
DT   25-MAY-2022, entry version 23.
DE   RecName: Full=Spindle pole body component KRE28;
GN   Name=KRE28; ORFNames=C1Q_00785;
OS   Saccharomyces cerevisiae (strain JAY291) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=574961;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JAY291;
RX   PubMed=19812109; DOI=10.1101/gr.091777.109;
RA   Argueso J.L., Carazzolle M.F., Mieczkowski P.A., Duarte F.M., Netto O.V.C.,
RA   Missawa S.K., Galzerani F., Costa G.G.L., Vidal R.O., Noronha M.F.,
RA   Dominska M., Andrietta M.G.S., Andrietta S.R., Cunha A.F., Gomes L.H.,
RA   Tavares F.C.A., Alcarde A.R., Dietrich F.S., McCusker J.H., Petes T.D.,
RA   Pereira G.A.G.;
RT   "Genome structure of a Saccharomyces cerevisiae strain widely used in
RT   bioethanol production.";
RL   Genome Res. 19:2258-2270(2009).
CC   -!- FUNCTION: Forms a kinetochore complex with SPC105 which is required for
CC       kinetochore binding by a discrete subset of kMAPs (BIM1, BIK1 and
CC       SLK19) and motors (CIN8, KAR3). Involved in kinetochore-microtubule
CC       binding and the spindle assembly checkpoint (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Component of the SPC105 complex composed of at least SPC105
CC       and KRE28. The SPC105 complex interacts with the MIND and NDC80
CC       complexes at the centromere (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC       center, spindle pole body {ECO:0000250}. Nucleus membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Nucleoplasmic
CC       side {ECO:0000250}. Chromosome, centromere, kinetochore {ECO:0000250}.
CC       Note=Localizes to the nuclear side of the spindle pole body.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the KRE28 family. {ECO:0000305}.
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DR   EMBL; ACFL01000026; EEU08564.1; -; Genomic_DNA.
DR   AlphaFoldDB; C7GKW3; -.
DR   SMR; C7GKW3; -.
DR   Proteomes; UP000008073; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR   GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005816; C:spindle pole body; IEA:UniProtKB-SubCell.
DR   InterPro; IPR031361; Kre28.
DR   Pfam; PF17097; Kre28; 1.
PE   3: Inferred from homology;
KW   Centromere; Chromosome; Coiled coil; Cytoplasm; Cytoskeleton; Kinetochore;
KW   Membrane; Nucleus.
FT   CHAIN           1..385
FT                   /note="Spindle pole body component KRE28"
FT                   /id="PRO_0000408566"
FT   COILED          128..175
FT                   /evidence="ECO:0000255"
FT   COILED          229..258
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   385 AA;  44674 MW;  4ED8E3B2A55A11D1 CRC64;
     MDTGSASIKD YETVLTDIED SIAVSSEEVL NNQELRLKNT LHEITSSILA INEENKFVNP
     LRNDESLDVE GKEVFVNPKI LSAKIKEFNK LMELLKLTYL EQETLDYFFR FTLSSTKPLQ
     LDSEKDPQFV KLNERVNDLK EEISNVQESK IEQIKAEIQE TGHNFAEKQD LINELYLEAT
     GDIENCWDSL NELKNLTNKE DKNMMGEKDT ILNSSDSDDF VEETYTNWQK LLFLQKQNQR
     LTKELKEMHE VKNQIIRKGE QSKKEDSGHL MANESELCQS INLLTKFWEK HFLLKGSKTT
     ILNFEIFTQL GKVQFEIKDM QYIIAISLSD LKRPMIKDIT ILQKAGGNIV TDIEANSKFN
     NKYRNNTKVQ IFEVMDDIIS ELTNE