ID   KRE28_YEAST             Reviewed;         385 AA.
AC   Q04431; D6VTF3; Q6Q537;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 137.
DE   RecName: Full=Spindle pole body component KRE28;
GN   Name=KRE28; OrderedLocusNames=YDR532C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169867;
RA   Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA   Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA   Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA   Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA   Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA   Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA   Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA   Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA   Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA   Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA   Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA   Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA   Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA   Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA   Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA   Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA   Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA   Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA   Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA   Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA   Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA   Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA   Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA   Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA   Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA   Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA   Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA   Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA   Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA   Mewes H.-W., Zollner A., Zaccaria P.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL   Nature 387:75-78(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   SUBUNIT, INTERACTION WITH SPC105, AND SUBCELLULAR LOCATION.
RX   PubMed=14565975; DOI=10.1091/mbc.e03-06-0419;
RA   Nekrasov V.S., Smith M.A., Peak-Chew S., Kilmartin J.V.;
RT   "Interactions between centromere complexes in Saccharomyces cerevisiae.";
RL   Mol. Biol. Cell 14:4931-4946(2003).
RN   [5]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [6]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [7]
RP   INTERACTION WITH SPC105, AND FUNCTION.
RX   PubMed=19893618; DOI=10.1371/journal.pone.0007640;
RA   Pagliuca C., Draviam V.M., Marco E., Sorger P.K., De Wulf P.;
RT   "Roles for the conserved spc105p/kre28p complex in kinetochore-microtubule
RT   binding and the spindle assembly checkpoint.";
RL   PLoS ONE 4:E7640-E7640(2009).
CC   -!- FUNCTION: Forms a kinetochore complex with SPC105 which is required for
CC       kinetochore binding by a discrete subset of kMAPs (BIM1, BIK1 and
CC       SLK19) and motors (CIN8, KAR3). Involved in kinetochore-microtubule
CC       binding and the spindle assembly checkpoint.
CC       {ECO:0000269|PubMed:19893618}.
CC   -!- SUBUNIT: Component of the SPC105 complex composed of at least SPC105
CC       and KRE28. The SPC105 complex interacts with the MIND and NDC80
CC       complexes at the centromere. {ECO:0000269|PubMed:14565975,
CC       ECO:0000269|PubMed:19893618}.
CC   -!- INTERACTION:
CC       Q04431; P53148: SPC105; NbExp=3; IntAct=EBI-32446, EBI-23870;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC       center, spindle pole body. Nucleus membrane; Peripheral membrane
CC       protein; Nucleoplasmic side. Chromosome, centromere, kinetochore.
CC       Note=Localizes to the nuclear side of the spindle pole body.
CC   -!- MISCELLANEOUS: Present with 377 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the KRE28 family. {ECO:0000305}.
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DR   EMBL; U33057; AAB64971.1; -; Genomic_DNA.
DR   EMBL; AY558580; AAS56906.1; -; Genomic_DNA.
DR   EMBL; BK006938; DAA12363.1; -; Genomic_DNA.
DR   PIR; S69587; S69587.
DR   RefSeq; NP_010821.3; NM_001180840.3.
DR   AlphaFoldDB; Q04431; -.
DR   SMR; Q04431; -.
DR   BioGRID; 32581; 123.
DR   ComplexPortal; CPX-1899; Spc105 complex.
DR   DIP; DIP-1393N; -.
DR   IntAct; Q04431; 10.
DR   MINT; Q04431; -.
DR   STRING; 4932.YDR532C; -.
DR   iPTMnet; Q04431; -.
DR   MaxQB; Q04431; -.
DR   PaxDb; Q04431; -.
DR   PRIDE; Q04431; -.
DR   EnsemblFungi; YDR532C_mRNA; YDR532C; YDR532C.
DR   GeneID; 852145; -.
DR   KEGG; sce:YDR532C; -.
DR   SGD; S000002940; KRE28.
DR   VEuPathDB; FungiDB:YDR532C; -.
DR   eggNOG; ENOG502S19U; Eukaryota.
DR   HOGENOM; CLU_062083_0_0_1; -.
DR   InParanoid; Q04431; -.
DR   OMA; LADECHT; -.
DR   BioCyc; YEAST:G3O-30043-MON; -.
DR   PRO; PR:Q04431; -.
DR   Proteomes; UP000002311; Chromosome IV.
DR   RNAct; Q04431; protein.
DR   GO; GO:0000775; C:chromosome, centromeric region; IDA:SGD.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0000776; C:kinetochore; IDA:SGD.
DR   GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005816; C:spindle pole body; IEA:UniProtKB-SubCell.
DR   GO; GO:0051382; P:kinetochore assembly; IC:ComplexPortal.
DR   InterPro; IPR031361; Kre28.
DR   Pfam; PF17097; Kre28; 1.
PE   1: Evidence at protein level;
KW   Centromere; Chromosome; Coiled coil; Cytoplasm; Cytoskeleton; Kinetochore;
KW   Membrane; Nucleus; Reference proteome.
FT   CHAIN           1..385
FT                   /note="Spindle pole body component KRE28"
FT                   /id="PRO_0000253889"
FT   COILED          128..167
FT                   /evidence="ECO:0000255"
FT   COILED          229..258
FT                   /evidence="ECO:0000255"
FT   CONFLICT        263
FT                   /note="K -> R (in Ref. 3; AAS56906)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   385 AA;  44675 MW;  A1A44CF0D9E75F50 CRC64;
     MDTGSASIKD YETVLTDIED SIAVSSEEVL NNQELRLKNT LHEITSSILA INEENKFVNP
     LRNDESLDVE GKEVFVNPKI LSAKIKEFNK LMELLKLTYL EQETLDYFFR FTLSSTKPLQ
     LDSEKDPQFV KLNERVNDLK EEISNVQESK IEQIKAEIQE TGHNFAERQD LINELYLEAT
     GDIENCWDSL NELKNLTNKE DKNMMGEKDT ILNSSDSDDF VEETYTNWQK LLFLQKQNQR
     LTKELKEMHE VKNQIIRKGE QSKKEDSGHL MANESELCQS INLLTKFWEK HFLLKGSKTT
     ILNFEIFTQL GKVQFEIKDM QYIIAISLSD LKRPMIKDIT ILQKAGGNIV TDIEASSKFN
     NKYRNNTKVQ IFEVMDDIIS ELTNE