ARC2_DROME
ID ARC2_DROME Reviewed; 193 AA.
AC Q7JV70;
DT 28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Activity-regulated cytoskeleton associated protein 2 {ECO:0000303|PubMed:29328915};
DE Short=dArc2 {ECO:0000303|PubMed:29328915};
GN Name=Arc2 {ECO:0000303|PubMed:29328915, ECO:0000312|FlyBase:FBgn0033928};
GN ORFNames=CG13941 {ECO:0000312|FlyBase:FBgn0033928};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Celniker S., Carlson J., Wan K., Pfeiffer B., Frise E., George R.,
RA Hoskins R., Stapleton M., Pacleb J., Park S., Svirskas R., Smith E., Yu C.,
RA Rubin G.;
RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=29328915; DOI=10.1016/j.cell.2017.12.022;
RA Ashley J., Cordy B., Lucia D., Fradkin L.G., Budnik V., Thomson T.;
RT "Retrovirus-like Gag protein Arc1 binds RNA and traffics across synaptic
RT boutons.";
RL Cell 172:262-274(2018).
CC -!- FUNCTION: Self-assembles into virion-like capsids that encapsulate RNAs
CC and mediate intercellular RNA transfer. Arc2 protein is released from
CC cells in extracellular vesicles that mediate the transfer of mRNA into
CC neighboring cells. {ECO:0000250|UniProtKB:Q7K1U0}.
CC -!- SUBUNIT: Homooligomer; homooligomerizes into virion-like capsids.
CC {ECO:0000250|UniProtKB:Q63053}.
CC -!- INTERACTION:
CC Q7JV70; Q7K1U0: Arc1; NbExp=4; IntAct=EBI-172533, EBI-103780;
CC Q7JV70; Q7JV70: Arc2; NbExp=4; IntAct=EBI-172533, EBI-172533;
CC -!- SUBCELLULAR LOCATION: Extracellular vesicle membrane
CC {ECO:0000269|PubMed:29328915}. Note=Forms virion-like extracellular
CC vesicles. {ECO:0000269|PubMed:29328915}.
CC -!- DOMAIN: The protein is evolutionarily related to retrotransposon Gag
CC proteins. It contains structural elements found within viral Gag
CC polyproteins originated from the Ty3/gypsy retrotransposon family and
CC retains the ability to form virion-like capsid structures that can
CC mediate mRNA transfer between cells. Tetrapod and fly Arc protein-
CC coding genes originated independently from distinct lineages of
CC Ty3/gypsy retrotransposons. {ECO:0000250|UniProtKB:Q7K1U0}.
CC -!- SIMILARITY: Belongs to the ARC/ARG3.1 family. {ECO:0000305}.
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DR EMBL; AE013599; AAF58254.1; -; Genomic_DNA.
DR EMBL; AY119191; AAM51051.1; -; mRNA.
DR RefSeq; NP_610956.1; NM_137112.2.
DR PDB; 6SIB; X-ray; 1.90 A; A=38-109.
DR PDB; 6SIE; X-ray; 2.80 A; A/B/C/D=110-193.
DR PDB; 6TAQ; EM; 3.90 A; A/B/C/D=1-193.
DR PDB; 6TAT; EM; 3.70 A; A/B/C/D/E=1-193.
DR PDB; 6TAU; EM; 3.70 A; A/B/C/D/E/F=1-193.
DR PDBsum; 6SIB; -.
DR PDBsum; 6SIE; -.
DR PDBsum; 6TAQ; -.
DR PDBsum; 6TAT; -.
DR PDBsum; 6TAU; -.
DR AlphaFoldDB; Q7JV70; -.
DR SMR; Q7JV70; -.
DR IntAct; Q7JV70; 2.
DR STRING; 7227.FBpp0086675; -.
DR PaxDb; Q7JV70; -.
DR PRIDE; Q7JV70; -.
DR DNASU; 36597; -.
DR EnsemblMetazoa; FBtr0087547; FBpp0086675; FBgn0033928.
DR GeneID; 36597; -.
DR KEGG; dme:Dmel_CG13941; -.
DR UCSC; CG13941-RA; d. melanogaster.
DR CTD; 36597; -.
DR FlyBase; FBgn0033928; Arc2.
DR VEuPathDB; VectorBase:FBgn0033928; -.
DR eggNOG; ENOG502SQYT; Eukaryota.
DR GeneTree; ENSGT00540000073579; -.
DR HOGENOM; CLU_097305_0_0_1; -.
DR InParanoid; Q7JV70; -.
DR OMA; YMEIFET; -.
DR OrthoDB; 1169210at2759; -.
DR PhylomeDB; Q7JV70; -.
DR BioGRID-ORCS; 36597; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36597; -.
DR PRO; PR:Q7JV70; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0033928; Expressed in midgut and 26 other tissues.
DR GO; GO:1903561; C:extracellular vesicle; IDA:UniProtKB.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR InterPro; IPR045358; Ty3_capsid.
DR Pfam; PF19259; Ty3_capsid; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Membrane; Reference proteome; RNA-binding; Transport.
FT CHAIN 1..193
FT /note="Activity-regulated cytoskeleton associated protein
FT 2"
FT /id="PRO_0000443802"
FT HELIX 47..61
FT /evidence="ECO:0007829|PDB:6SIB"
FT HELIX 66..90
FT /evidence="ECO:0007829|PDB:6SIB"
FT HELIX 95..105
FT /evidence="ECO:0007829|PDB:6SIB"
FT HELIX 112..121
FT /evidence="ECO:0007829|PDB:6SIE"
FT HELIX 130..143
FT /evidence="ECO:0007829|PDB:6SIE"
FT HELIX 151..159
FT /evidence="ECO:0007829|PDB:6SIE"
FT HELIX 164..169
FT /evidence="ECO:0007829|PDB:6SIE"
FT HELIX 172..174
FT /evidence="ECO:0007829|PDB:6SIE"
FT HELIX 178..190
FT /evidence="ECO:0007829|PDB:6SIE"
SQ SEQUENCE 193 AA; 22622 MW; 5841D186C065D391 CRC64;
MTQMSDEQFR IFIETIKSLG PIKEEPPSKG SFSNCTVRFS GQRDHDAVDE FINAVETYKE
VEGISDKDAL KGLPLLFKSI AVVWWKGVRR DAKTWSDALQ LLRDHFSPTK PSYQIYMEIF
ETKQSYDEVI DSFICKQRAL LAKLPEGRHD EETELDFIYG LMQPKYRESI PRHEVKTFRE
LLDRGRTVER TRH
