KRP1_ARATH
ID KRP1_ARATH Reviewed; 191 AA.
AC Q67Y93; O04154; O82809; Q8LDX1;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 2.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Cyclin-dependent kinase inhibitor 1;
DE AltName: Full=Inhibitor/interactor of CDK protein 1;
DE AltName: Full=KIP-related protein 1;
GN Name=KRP1; Synonyms=ICK1; OrderedLocusNames=At2g23430; ORFNames=F26B6.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND INTERACTION WITH CDKA-1.
RC STRAIN=cv. Columbia;
RX PubMed=9087400; DOI=10.1038/386451a0;
RA Wang H., Fowke L.C., Crosby W.L.;
RT "A plant cyclin-dependent kinase inhibitor gene.";
RL Nature 386:451-452(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION,
RP AND INTERACTION WITH CDKA-1 AND CYCD3-1.
RC STRAIN=cv. Columbia;
RX PubMed=9753775; DOI=10.1046/j.1365-313x.1998.00231.x;
RA Wang H., Qi Q., Schorr P., Cutler A.J., Crosby W.L., Fowke L.C.;
RT "ICK1, a cyclin-dependent protein kinase inhibitor from Arabidopsis
RT thaliana interacts with both Cdc2a and CycD3, and its expression is induced
RT by abscisic acid.";
RL Plant J. 15:501-510(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP FUNCTION.
RX PubMed=11123800; DOI=10.1046/j.1365-313x.2000.00899.x;
RA Wang H., Zhou Y., Gilmer S., Whitwill S., Fowke L.C.;
RT "Expression of the plant cyclin-dependent kinase inhibitor ICK1 affects
RT cell division, plant growth and morphology.";
RL Plant J. 24:613-623(2000).
RN [9]
RP INTERACTION WITH CDKA-1.
RX PubMed=11449057; DOI=10.2307/3871392;
RA de Veylder L., Beeckman T., Beemster G.T.S., Krols L., Terras F.,
RA Landrieu I., van der Schueren E., Maes S., Naudts M., Inze D.;
RT "Functional analysis of cyclin-dependent kinase inhibitors of
RT Arabidopsis.";
RL Plant Cell 13:1653-1667(2001).
RN [10]
RP FUNCTION.
RX PubMed=12029474; DOI=10.1007/s00425-002-0752-2;
RA Zhou Y., Wang H., Gilmer S., Whitwill S., Keller W., Fowke L.C.;
RT "Control of petal and pollen development by the plant cyclin-dependent
RT kinase inhibitor ICK1 in transgenic Brassica plants.";
RL Planta 215:248-257(2002).
RN [11]
RP DEVELOPMENTAL STAGE.
RX PubMed=12000456; DOI=10.1046/j.1365-313x.2002.01274.x;
RA Menges M., Murray J.A.H.;
RT "Synchronous Arabidopsis suspension cultures for analysis of cell-cycle
RT gene activity.";
RL Plant J. 30:203-212(2002).
RN [12]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11971144; DOI=10.1105/tpc.010445;
RA Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D.;
RT "Genome-wide analysis of core cell cycle genes in Arabidopsis.";
RL Plant Cell 14:903-916(2002).
RN [13]
RP DEVELOPMENTAL STAGE.
RX PubMed=12368490; DOI=10.1105/tpc.004960;
RA Himanen K., Boucheron E., Vanneste S., de Almeida Engler J., Inze D.,
RA Beeckman T.;
RT "Auxin-mediated cell cycle activation during early lateral root
RT initiation.";
RL Plant Cell 14:2339-2351(2002).
RN [14]
RP INTERACTION WITH CYCD2-1.
RX PubMed=12569402; DOI=10.1007/s00425-002-0935-x;
RA Zhou Y., Wang H., Gilmer S., Whitwill S., Fowke L.C.;
RT "Effects of co-expressing the plant CDK inhibitor ICK1 and D-type cyclin
RT genes on plant growth, cell size and ploidy in Arabidopsis thaliana.";
RL Planta 216:604-613(2003).
RN [15]
RP FUNCTION, AND INTERACTION WITH CDKA-1 AND CYCD3-1.
RX PubMed=12566574; DOI=10.1105/tpc.008342;
RA Schnittger A., Weinl C., Bouyer D., Schoebinger U., Huelskamp M.;
RT "Misexpression of the cyclin-dependent kinase inhibitor ICK1/KRP1 in
RT single-celled Arabidopsis trichomes reduces endoreduplication and cell size
RT and induces cell death.";
RL Plant Cell 15:303-315(2003).
RN [16]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=12904210; DOI=10.1046/j.1365-313x.2003.01821.x;
RA Zhou Y., Li G., Brandizzi F., Fowke L.C., Wang H.;
RT "The plant cyclin-dependent kinase inhibitor ICK1 has distinct functional
RT domains for in vivo kinase inhibition, protein instability and nuclear
RT localization.";
RL Plant J. 35:476-489(2003).
RN [17]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15749764; DOI=10.1105/tpc.104.030486;
RA Weinl C., Marquardt S., Kuijt S.J.H., Nowack M.K., Jakoby M.J.,
RA Huelskamp M., Schnittger A.;
RT "Novel functions of plant cyclin-dependent kinase inhibitors, ICK1/KRP1,
RT can act non-cell-autonomously and inhibit entry into mitosis.";
RL Plant Cell 17:1704-1722(2005).
RN [18]
RP FUNCTION.
RX PubMed=16376885; DOI=10.1016/j.febslet.2005.12.018;
RA Nakai T., Kato K., Shinmyo A., Sekine M.;
RT "Arabidopsis KRPs have distinct inhibitory activity toward cyclin D2-
RT associated kinases, including plant-specific B-type cyclin-dependent
RT kinase.";
RL FEBS Lett. 580:336-340(2006).
RN [19]
RP SUBCELLULAR LOCATION, INTERACTION WITH CDKA-1, AND MUTAGENESIS OF LYS-84
RP AND LYS-86.
RX PubMed=16845478; DOI=10.1007/s11103-006-9019-9;
RA Zhou Y., Niu H., Brandizzi F., Fowke L.C., Wang H.;
RT "Molecular control of nuclear and subnuclear targeting of the plant CDK
RT inhibitor ICK1 and ICK1-mediated nuclear transport of CDKA.";
RL Plant Mol. Biol. 62:261-278(2006).
RN [20]
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF ARG-80; ARG-81; LYS-84
RP AND LYS-86.
RX PubMed=16766674; DOI=10.1104/pp.106.081406;
RA Jakoby M.J., Weinl C., Pusch S., Kuijt S.J.H., Merkle T., Dissmeyer N.,
RA Schnittger A.;
RT "Analysis of the subcellular localization, function, and proteolytic
RT control of the Arabidopsis cyclin-dependent kinase inhibitor ICK1/KRP1.";
RL Plant Physiol. 141:1293-1305(2006).
RN [21]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=17253089; DOI=10.1007/s00299-006-0294-3;
RA Bird D.A., Buruiana M.M., Zhou Y., Fowke L.C., Wang H.;
RT "Arabidopsis cyclin-dependent kinase inhibitors are nuclear-localized and
RT show different localization patterns within the nucleoplasm.";
RL Plant Cell Rep. 26:861-872(2007).
RN [22]
RP TISSUE SPECIFICITY, INTERACTION WITH CDKA-1 AND CYCD2-1, UBIQUITINATION,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=18005227; DOI=10.1111/j.1365-313x.2007.03370.x;
RA Ren H., Santner A., del Pozo J.C., Murray J.A., Estelle M.;
RT "Degradation of the cyclin-dependent kinase inhibitor KRP1 is regulated by
RT two different ubiquitin E3 ligases.";
RL Plant J. 53:705-716(2008).
CC -!- FUNCTION: Binds and inhibits CYCD2-1/CDKA-1 kinase complex activity.
CC Regulates cell division which is crucial for plant growth, development
CC and morphogenesis. Functions in turning cells from a mitotic to an
CC endoreplicating cell cycle mode. Acts cell- and non-cell-autonomously
CC to regulate endoreduplication by allowing S phase progression, but
CC blocking entry into mitosis. Keeps on the one hand the plant cell cycle
CC locally controlled, and on the other hand provides a possibility of
CC linking cell cycle control in single cells with the supracellular
CC organization of a tissue or an organ. May target specifically CDKA-1.
CC {ECO:0000269|PubMed:11123800, ECO:0000269|PubMed:12029474,
CC ECO:0000269|PubMed:12566574, ECO:0000269|PubMed:12904210,
CC ECO:0000269|PubMed:15749764, ECO:0000269|PubMed:16376885,
CC ECO:0000269|PubMed:16766674, ECO:0000269|PubMed:17253089,
CC ECO:0000269|PubMed:9087400, ECO:0000269|PubMed:9753775}.
CC -!- SUBUNIT: Specifically interacts with CDKA-1, but not with CDKB1-1.
CC Interacts with CYCD2-1 and CYCD3-1. {ECO:0000269|PubMed:11449057,
CC ECO:0000269|PubMed:12566574, ECO:0000269|PubMed:12569402,
CC ECO:0000269|PubMed:16845478, ECO:0000269|PubMed:18005227,
CC ECO:0000269|PubMed:9087400, ECO:0000269|PubMed:9753775}.
CC -!- INTERACTION:
CC Q67Y93; P24100: CDKA-1; NbExp=10; IntAct=EBI-1636730, EBI-371713;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000269|PubMed:12904210, ECO:0000269|PubMed:15749764,
CC ECO:0000269|PubMed:16766674, ECO:0000269|PubMed:16845478,
CC ECO:0000269|PubMed:17253089}. Note=Distributed in a ponctuate pattern
CC and in heterochromatic chromocenters.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in roots, stems, leaves and
CC flowers. {ECO:0000269|PubMed:18005227, ECO:0000269|PubMed:9753775}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in root pericycle and cell
CC suspension culture during cell cycle arrest. Expressed early in the G1
CC phase, disappears quickly and starts to increase again to reach a peak
CC before mitosis. {ECO:0000269|PubMed:12000456,
CC ECO:0000269|PubMed:12368490}.
CC -!- INDUCTION: By abscisic acid (ABA). {ECO:0000269|PubMed:9753775}.
CC -!- PTM: Ubiquitinated independently by RKP and SCF (SKP1-CUL1-FBL5/SKP2B)
CC protein ligase complex, leading to proteasomal degradation.
CC {ECO:0000269|PubMed:18005227}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC {ECO:0000269|PubMed:18005227}.
CC -!- MISCELLANEOUS: Plants overexpressing ICK1/KRP1 show altered morphology
CC (including flowers) with a reduction in plant size, cell numbers,
CC pollen germination, seed production and CDK kinase activity. Trichome
CC cells misexpressing ICK1/KRP1 show reduced endoreduplication and cell
CC size, and undergo cell death. ICK1/KRP1 degradation is regulated by the
CC AXR1-dependent RUB conjugation pathway that is required for the
CC function of the SCF complexes.
CC -!- SIMILARITY: Belongs to the CDI family. ICK/KRP subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD43255.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAD44338.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; U94772; AAC49698.1; -; mRNA.
DR EMBL; AF079587; AAC34660.1; -; Genomic_DNA.
DR EMBL; AC003040; AAC23758.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07454.1; -; Genomic_DNA.
DR EMBL; AK175492; BAD43255.1; ALT_FRAME; mRNA.
DR EMBL; AK176575; BAD44338.1; ALT_FRAME; mRNA.
DR EMBL; BT028986; ABI93895.1; -; mRNA.
DR EMBL; AY085749; AAM62967.1; -; mRNA.
DR PIR; T01132; T01132.
DR RefSeq; NP_179924.1; NM_127907.4.
DR AlphaFoldDB; Q67Y93; -.
DR SMR; Q67Y93; -.
DR BioGRID; 2227; 27.
DR IntAct; Q67Y93; 6.
DR STRING; 3702.AT2G23430.1; -.
DR PaxDb; Q67Y93; -.
DR PRIDE; Q67Y93; -.
DR ProteomicsDB; 237100; -.
DR EnsemblPlants; AT2G23430.1; AT2G23430.1; AT2G23430.
DR GeneID; 816875; -.
DR Gramene; AT2G23430.1; AT2G23430.1; AT2G23430.
DR KEGG; ath:AT2G23430; -.
DR Araport; AT2G23430; -.
DR TAIR; locus:2046778; AT2G23430.
DR HOGENOM; CLU_083146_1_0_1; -.
DR InParanoid; Q67Y93; -.
DR OMA; RRIVYVR; -.
DR PRO; PR:Q67Y93; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q67Y93; baseline and differential.
DR Genevisible; Q67Y93; AT.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0004861; F:cyclin-dependent protein serine/threonine kinase inhibitor activity; IDA:TAIR.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0048527; P:lateral root development; IMP:TAIR.
DR GO; GO:0010311; P:lateral root formation; IMP:TAIR.
DR GO; GO:0048366; P:leaf development; IMP:TAIR.
DR GO; GO:0045736; P:negative regulation of cyclin-dependent protein serine/threonine kinase activity; IDA:TAIR.
DR GO; GO:0045740; P:positive regulation of DNA replication; IMP:TAIR.
DR GO; GO:0009737; P:response to abscisic acid; IEP:TAIR.
DR GO; GO:0048367; P:shoot system development; IMP:TAIR.
DR Gene3D; 4.10.365.10; -; 1.
DR InterPro; IPR003175; CDI_dom.
DR InterPro; IPR044898; CDI_dom_sf.
DR InterPro; IPR044275; KRP.
DR PANTHER; PTHR46776; PTHR46776; 1.
DR Pfam; PF02234; CDI; 1.
DR PIRSF; PIRSF017811; CDK_inhib_pln; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Nucleus; Protein kinase inhibitor; Reference proteome;
KW Ubl conjugation.
FT CHAIN 1..191
FT /note="Cyclin-dependent kinase inhibitor 1"
FT /id="PRO_0000294087"
FT REGION 62..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 162..191
FT /note="Required for inhibitory function and interaction
FT with CDK kinase complexes"
FT MUTAGEN 80
FT /note="R->A: Slightly affects transport to nucleus; when
FT associated with A-81."
FT /evidence="ECO:0000269|PubMed:16766674"
FT MUTAGEN 81
FT /note="R->A: Slightly affects transport to nucleus; when
FT associated with A-80."
FT /evidence="ECO:0000269|PubMed:16766674"
FT MUTAGEN 84
FT /note="K->A: Affects transport to nucleus, but does not
FT abolish it; when associated with A-86."
FT /evidence="ECO:0000269|PubMed:16766674,
FT ECO:0000269|PubMed:16845478"
FT MUTAGEN 86
FT /note="K->A: Affects transport to nucleus, but does not
FT abolish it; when associated with A-84."
FT /evidence="ECO:0000269|PubMed:16766674,
FT ECO:0000269|PubMed:16845478"
FT CONFLICT 88
FT /note="F -> C (in Ref. 1; AAC49698)"
FT /evidence="ECO:0000305"
FT CONFLICT 191
FT /note="E -> Q (in Ref. 7; AAM62967)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 191 AA; 22283 MW; 0477A91E277C46B2 CRC64;
MVRKYRKAKG IVEAGVSSTY MQLRSRRIVY VRSEKSSSVS VVGDNGVSSS CSGSNEYKKK
ELIHLEEEDK DGDTETSTYR RGTKRKLFEN LREEEKEELS KSMENYSSEF ESAVKESLDC
CCSGRKTMEE TVTAEEEEKA KLMTEMPTES EIEDFFVEAE KQLKEKFKKK YNFDFEKEKP
LEGRYEWVKL E
