ID   KRP3_ARATH              Reviewed;         222 AA.
AC   Q9FKB5;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Cyclin-dependent kinase inhibitor 3;
DE   AltName: Full=Inhibitor/interactor of CDK protein 6;
DE   AltName: Full=KIP-related protein 3;
GN   Name=KRP3; Synonyms=ICK6; OrderedLocusNames=At5g48820; ORFNames=K24G6.15;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, AND INTERACTION WITH
RP   CDKA-1.
RC   STRAIN=cv. Columbia;
RX   PubMed=11449057; DOI=10.2307/3871392;
RA   de Veylder L., Beeckman T., Beemster G.T.S., Krols L., Terras F.,
RA   Landrieu I., van der Schueren E., Maes S., Naudts M., Inze D.;
RT   "Functional analysis of cyclin-dependent kinase inhibitors of
RT   Arabidopsis.";
RL   Plant Cell 13:1653-1667(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA   Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT   features of the regions of 1,367,185 bp covered by 19 physically assigned
RT   P1 and TAC clones.";
RL   DNA Res. 5:203-216(1998).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=12000456; DOI=10.1046/j.1365-313x.2002.01274.x;
RA   Menges M., Murray J.A.H.;
RT   "Synchronous Arabidopsis suspension cultures for analysis of cell-cycle
RT   gene activity.";
RL   Plant J. 30:203-212(2002).
RN   [6]
RP   DEVELOPMENTAL STAGE, AND INDUCTION.
RX   PubMed=12368490; DOI=10.1105/tpc.004960;
RA   Himanen K., Boucheron E., Vanneste S., de Almeida Engler J., Inze D.,
RA   Beeckman T.;
RT   "Auxin-mediated cell cycle activation during early lateral root
RT   initiation.";
RL   Plant Cell 14:2339-2351(2002).
RN   [7]
RP   FUNCTION.
RX   PubMed=16376885; DOI=10.1016/j.febslet.2005.12.018;
RA   Nakai T., Kato K., Shinmyo A., Sekine M.;
RT   "Arabidopsis KRPs have distinct inhibitory activity toward cyclin D2-
RT   associated kinases, including plant-specific B-type cyclin-dependent
RT   kinase.";
RL   FEBS Lett. 580:336-340(2006).
RN   [8]
RP   SUBCELLULAR LOCATION.
RX   PubMed=17253089; DOI=10.1007/s00299-006-0294-3;
RA   Bird D.A., Buruiana M.M., Zhou Y., Fowke L.C., Wang H.;
RT   "Arabidopsis cyclin-dependent kinase inhibitors are nuclear-localized and
RT   show different localization patterns within the nucleoplasm.";
RL   Plant Cell Rep. 26:861-872(2007).
CC   -!- FUNCTION: Binds and inhibits CYCD2-1/CDKA-1 complex kinase activity.
CC       May target specifically CDKA-1. {ECO:0000269|PubMed:16376885}.
CC   -!- SUBUNIT: Specifically interacts with CDKA-1, but not with CDKB1-1.
CC       {ECO:0000269|PubMed:11449057}.
CC   -!- INTERACTION:
CC       Q9FKB5; P24100: CDKA-1; NbExp=4; IntAct=EBI-1773302, EBI-371713;
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC       {ECO:0000269|PubMed:17253089}. Note=Distributed in a ponctuate pattern.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences.;
CC       Name=1;
CC         IsoId=Q9FKB5-1; Sequence=Displayed;
CC   -!- DEVELOPMENTAL STAGE: Highly expressed in actively dividing cells of
CC       root pericycle and suspension cell culture. Expressed in the G1/S
CC       phases and reaches a peak at the G2 phase.
CC       {ECO:0000269|PubMed:11449057, ECO:0000269|PubMed:12000456,
CC       ECO:0000269|PubMed:12368490}.
CC   -!- INDUCTION: By auxin in roots. {ECO:0000269|PubMed:12368490}.
CC   -!- MISCELLANEOUS: Treatment with auxin induces lateral root initiation.
CC   -!- SIMILARITY: Belongs to the CDI family. ICK/KRP subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AJ301554; CAC41617.1; -; mRNA.
DR   EMBL; AB012242; BAB09435.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED95728.1; -; Genomic_DNA.
DR   EMBL; BT025290; ABF19043.1; -; mRNA.
DR   RefSeq; NP_199693.1; NM_124259.2. [Q9FKB5-1]
DR   AlphaFoldDB; Q9FKB5; -.
DR   BioGRID; 20186; 28.
DR   IntAct; Q9FKB5; 13.
DR   STRING; 3702.AT5G48820.2; -.
DR   iPTMnet; Q9FKB5; -.
DR   PRIDE; Q9FKB5; -.
DR   EnsemblPlants; AT5G48820.1; AT5G48820.1; AT5G48820. [Q9FKB5-1]
DR   GeneID; 834940; -.
DR   Gramene; AT5G48820.1; AT5G48820.1; AT5G48820. [Q9FKB5-1]
DR   KEGG; ath:AT5G48820; -.
DR   Araport; AT5G48820; -.
DR   eggNOG; ENOG502QXA1; Eukaryota.
DR   HOGENOM; CLU_083146_0_1_1; -.
DR   InParanoid; Q9FKB5; -.
DR   OMA; DRETIPC; -.
DR   PhylomeDB; Q9FKB5; -.
DR   PRO; PR:Q9FKB5; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FKB5; baseline and differential.
DR   Genevisible; Q9FKB5; AT.
DR   GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004861; F:cyclin-dependent protein serine/threonine kinase inhibitor activity; IEA:InterPro.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   Gene3D; 4.10.365.10; -; 1.
DR   InterPro; IPR003175; CDI_dom.
DR   InterPro; IPR044898; CDI_dom_sf.
DR   InterPro; IPR044275; KRP.
DR   PANTHER; PTHR46776; PTHR46776; 1.
DR   Pfam; PF02234; CDI; 1.
DR   PIRSF; PIRSF017811; CDK_inhib_pln; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cell cycle; Nucleus; Protein kinase inhibitor;
KW   Reference proteome.
FT   CHAIN           1..222
FT                   /note="Cyclin-dependent kinase inhibitor 3"
FT                   /id="PRO_0000294092"
FT   REGION          68..101
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   222 AA;  24925 MW;  7A3EB2C9A29688A7 CRC64;
     MGKYMKKSKI TGDISVMEVS KATAPSPGVR TRAAKTLALK RLNSSAADSA LPNDSSCYLQ
     LRSRRLEKPS SLIEPKQPPR VHRSGIKESG SRSRVDSVNS VPVAQSSNED ECFDNFVSVQ
     VSCGENSLGF ESRHSTREST PCNFVEDMEI MVTPGSSTRS MCRATKEYTR EQDNVIPTTS
     EMEEFFAYAE QQQQRLFMEK YNFDIVNDIP LSGRYEWVQV KP