KS1_ISOER
ID KS1_ISOER Reviewed; 794 AA.
AC A0A3G1QTS7;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 13-FEB-2019, sequence version 1.
DT 03-AUG-2022, entry version 15.
DE RecName: Full=Ent-kaurene synthase 1, chloroplastic {ECO:0000303|PubMed:30496917};
DE Short=IeKS1 {ECO:0000303|PubMed:30496917};
DE EC=4.2.3.19 {ECO:0000269|PubMed:30496917};
DE Flags: Precursor;
GN Name=KS1 {ECO:0000303|PubMed:30496917};
OS Isodon eriocalyx (Plectranthus eriocalyx).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Lamiales; Lamiaceae; Nepetoideae; Ocimeae; Isodoninae;
OC Isodon.
OX NCBI_TaxID=662907;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=30496917; DOI=10.1016/j.phytochem.2018.11.015;
RA Du G., Gong H.-Y., Feng K.-N., Chen Q.-Q., Yang Y.-L., Fu X.-L., Lu S.,
RA Zeng Y.;
RT "Diterpene synthases facilitating production of the kaurane skeleton of
RT eriocalyxin B in the medicinal plant Isodon eriocalyx.";
RL Phytochemistry 158:96-102(2019).
CC -!- FUNCTION: Involved in the biosynthesis of ent-kaurene diterpenoids
CC natural products such as oridonin, miltiradiene, eriocalyxin B and
CC nezukol, known to exhibit antitumor, anti-inflammatory and
CC antibacterial activities, and in the production of gibberellins
CC phytohormones (PubMed:30496917). Catalyzes the conversion of ent-
CC copalyl diphosphate (ent-CPP) to ent-kaurene (PubMed:30496917).
CC {ECO:0000269|PubMed:30496917}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ent-copalyl diphosphate = diphosphate + ent-kaur-16-ene;
CC Xref=Rhea:RHEA:22220, ChEBI:CHEBI:15415, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58553; EC=4.2.3.19;
CC Evidence={ECO:0000269|PubMed:30496917};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22221;
CC Evidence={ECO:0000269|PubMed:30496917};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:Q40577};
CC Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000250|UniProtKB:Q40577};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC {ECO:0000269|PubMed:30496917}.
CC -!- PATHWAY: Plant hormone biosynthesis; gibberellin biosynthesis.
CC {ECO:0000305|PubMed:30496917}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Accumulates in leaves, and, at low levels, in
CC germinating seeds. {ECO:0000269|PubMed:30496917}.
CC -!- DEVELOPMENTAL STAGE: Moslty expressed in leaves where ent-kaurane
CC diterpenoids accumulates but weakly expressed in germinating seeds in
CC which gibberellins are produced. {ECO:0000269|PubMed:30496917}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
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DR EMBL; KY937953; AWN06647.1; -; mRNA.
DR AlphaFoldDB; A0A3G1QTS7; -.
DR SMR; A0A3G1QTS7; -.
DR UniPathway; UPA00213; -.
DR UniPathway; UPA00390; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0009899; F:ent-kaurene synthase activity; IDA:UniProtKB.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0033332; P:ent-kaurene biosynthetic process; IDA:UniProtKB.
DR GO; GO:0009686; P:gibberellin biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IDA:UniProtKB.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SUPFAM; SSF48239; SSF48239; 2.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Lyase; Magnesium; Metal-binding; Plastid; Transit peptide.
FT TRANSIT 1..28
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 29..794
FT /note="Ent-kaurene synthase 1, chloroplastic"
FT /id="PRO_0000452388"
FT MOTIF 543..547
FT /note="DDXXD motif"
FT /evidence="ECO:0000305"
FT BINDING 543
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 543
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 547
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 547
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 687
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 695
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
SQ SEQUENCE 794 AA; 90262 MW; B4231EBFD07ADC48 CRC64;
MSLLLSNSVL VGPKFRSSRI SHASASLDIG LQRATSPQNA SVPTCFEETK GRIAKLFHKN
ELSVSTYDTA WVAMVPSPTS SEEPCFPACL NWLLENQCHD GSWARPHHHH MLKKDVLSST
LACILALKKW GVGEEQINRG LHFVELNFAS ATEKGQITPM GFDIIFPAML DNARGLSLNL
QLEPTMLNDL IYKRDLELKR CNQSNSAEKE VYWAHIAEGM GKLHDWESVM KYQRKNGSLF
NCPSTTAAAF TALRNSDCLN YLCLALEKFG SAVPAVYPLD IYSQLCTVGN LERLGISRYF
LTEIQSVLDE TYRSWLQGDE EIFMDASTCA LAFRTLRMNG YNVTSDPITK ILQECFSSSF
RGNMTDNNTT LEIYRASELI LYPEERDLVQ HNLRLKTFLE QELSSNGFIQ SCQLGRNINA
EVNQAIEYPF YAIMDRMAKR KNIENYNIDN TRILKTSYRS PNFGNKDFLS LSVEDFNRCQ
VIHREELREL ERWVIENRLD ELKFARSKAA YCYFSAAATI FSPELSDARM SWAKNGVLTT
VVDDFFDVGG SVEELKNLIQ LVELWDVDVS TQCCSPNVQI IFSALKHTIC EIADKGFKLQ
GRSITDHIIS IWLDLLYSMM KETELGIDKS FPTMDEYMSN AYVSFALGPI VLPALYLVGP
KLSEEMVNHS EYHTLFKLMS TCGRLLNDIR GYERELKDGK ISAVSLYIMN NGGEITTEAA
ISEMRSWIER DRRELLRLVL EENKSVLPKA CKKLFWHMCT VVHVFYSKDD GFTSLNLHGV
VNAIINEPIV LNQF
