KS1_ORYSJ
ID KS1_ORYSJ Reviewed; 756 AA.
AC Q0JA82; A0A0P0WEN8; Q2PHF3; Q69DT1; Q7XLE0;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Ent-kaur-16-ene synthase, chloroplastic;
DE EC=4.2.3.19;
DE AltName: Full=Ent-kaurene synthase B;
DE AltName: Full=Kaurene synthase 1;
DE Short=OsKS1;
DE Flags: Precursor;
GN Name=KS1; Synonyms=KS1A; OrderedLocusNames=Os04g0611800, LOC_Os04g52230;
GN ORFNames=OSJNBa0070C17.8;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Nipponbare;
RX PubMed=15668792; DOI=10.1007/s00299-004-0896-6;
RA Margis-Pinheiro M., Zhou X.-R., Zhu Q.-H., Dennis E.S., Upadhyaya N.M.;
RT "Isolation and characterization of a Ds-tagged rice (Oryza sativa L.) GA-
RT responsive dwarf mutant defective in an early step of the gibberellin
RT biosynthesis pathway.";
RL Plant Cell Rep. 23:819-833(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare;
RA Toyomasu T., Otomo K., Kanno Y., Mitsuhashi W., Sassa T.;
RT "Rice diterpene cyclases.";
RL Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP CATALYTIC ACTIVITY, AND MUTAGENESIS OF ILE-602.
RX PubMed=17456599; DOI=10.1073/pnas.0611454104;
RA Xu M., Wilderman P.R., Peters R.J.;
RT "Following evolution's lead to a single residue switch for diterpene
RT synthase product outcome.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:7397-7401(2007).
CC -!- FUNCTION: Catalyzes the conversion of ent-copalyl diphosphate to the
CC gibberellin precursor ent-kaur-16-ene.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ent-copalyl diphosphate = diphosphate + ent-kaur-16-ene;
CC Xref=Rhea:RHEA:22220, ChEBI:CHEBI:15415, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58553; EC=4.2.3.19;
CC Evidence={ECO:0000269|PubMed:17456599};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000250};
CC -!- PATHWAY: Plant hormone biosynthesis; gibberellin biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Highly expressed in panicles and at lower levels in
CC leaves and stems. {ECO:0000269|PubMed:15668792}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC -!- MISCELLANEOUS: Plants lacking KS1 display a dwarf phenotype with normal
CC root development, but severe stunting of shoots in seedlings, dark
CC green leaves and failure to initiate flowering. This phenotype can be
CC rescued by treatment with exogenous gibberellin.
CC -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE72099.1; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
CC Sequence=CAE05201.3; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY347876; AAQ72559.1; -; mRNA.
DR EMBL; AB126933; BAE72099.1; ALT_SEQ; mRNA.
DR EMBL; AL731610; CAE05201.3; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008210; BAF15755.1; -; Genomic_DNA.
DR EMBL; AP014960; BAS90962.1; -; Genomic_DNA.
DR AlphaFoldDB; Q0JA82; -.
DR SMR; Q0JA82; -.
DR STRING; 4530.OS04T0611800-00; -.
DR PaxDb; Q0JA82; -.
DR PRIDE; Q0JA82; -.
DR EnsemblPlants; Os04t0611800-00; Os04t0611800-00; Os04g0611800.
DR Gramene; Os04t0611800-00; Os04t0611800-00; Os04g0611800.
DR eggNOG; ENOG502QVGX; Eukaryota.
DR HOGENOM; CLU_003125_2_0_1; -.
DR InParanoid; Q0JA82; -.
DR OMA; DEYMTNG; -.
DR BRENDA; 4.2.3.19; 8948.
DR PlantReactome; R-OSA-1119348; Ent-kaurene biosynthesis.
DR UniPathway; UPA00390; -.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000059680; Chromosome 4.
DR Genevisible; Q0JA82; OS.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0009899; F:ent-kaurene synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000287; F:magnesium ion binding; IBA:GO_Central.
DR GO; GO:0010333; F:terpene synthase activity; IBA:GO_Central.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IBA:GO_Central.
DR GO; GO:0009686; P:gibberellin biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SUPFAM; SSF48239; SSF48239; 2.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Lyase; Magnesium; Metal-binding; Plastid; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..?
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN ?..756
FT /note="Ent-kaur-16-ene synthase, chloroplastic"
FT /id="PRO_0000372312"
FT MOTIF 496..500
FT /note="DDXXD motif"
FT BINDING 496
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 496
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 500
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 500
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 639
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 647
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT MUTAGEN 602
FT /note="I->T: Changes catalytic activity. Converts ent-
FT copalyl diphosphate to ent-pimara-8(14),15-diene and
FT diphosphate."
FT /evidence="ECO:0000269|PubMed:17456599"
FT CONFLICT 363
FT /note="C -> S (in Ref. 1; AAQ72559)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 756 AA; 85959 MW; 3D6E4772BB04AC75 CRC64;
MQHRKELQAR TRDQLQTLEL STSLYDTAWV AMVPLRGSRQ HPCFPQCVEW ILQNQQDDGS
WGTRGFGVAV TRDVLSSTLA CVLALKRWNV GQEHIRRGLD FIGRNFSIAM DEQIAAPVGF
NITFPGMLSL AMGMDLEFPV RQTDVDRLLH LREIELEREA GDHSYGRKAY MAYVTEGLGN
LLEWDEIMMF QRKNGSFFNC PSTTAATLVN HYNDKALQYL NCLVSKFGSA VPTVYPLNIY
CQLSWVDALE KMGISQYFVS EIKSILDTTY VSWLERDEEI MLDITTCAMA FRLLRMNGYH
VSSVELSPVA EASSFRESLQ GYLNDKKSLI ELYKASKVSK SENESILDSI GSWSGSLLKE
SVCSNGVKKA PIFEEMKYAL KFPFYTTLDR LDHKRNIERF DAKDSQMLKT EYLLPHANQD
ILALAVEDFS SSQSIYQDEL NYLECWVKDE KLDQLPFARQ KLTYCYLSAA ATIFPRELSE
ARIAWAKNGV LTTVVDDFFD LGGSKEELEN LIALVEKWDG HQEEFYSEQV RIVFSAIYTT
VNQLGAKASA LQGRDVTKHL TEIWLCLMRS MMTEAEWQRT KYVPTMEEYM ANAVVSFALG
PIVLPTLYFV GPKLQEDVVR DHEYNELFRL MSTCGRLLND SQGFERESLE GKLNSVSLLV
HHSGGSISID EAKMKAQKSI DTSRRNLLRL VLGEQGAVPR PCKQLFWKMC KIVHMFYSRT
DGFSSPKEMV SAVNAVVKEP LKLKVSDPYG SILSGN
