ID   ARCA2_RHIME             Reviewed;         419 AA.
AC   Q92YG5;
DT   25-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Arginine deiminase 2;
DE            Short=ADI 2;
DE            EC=3.5.3.6;
DE   AltName: Full=Arginine dihydrolase 2;
DE            Short=AD 2;
GN   Name=arcA2; OrderedLocusNames=RA0915; ORFNames=SMa1670;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OG   Plasmid pSymA (megaplasmid 1).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481432; DOI=10.1073/pnas.161294798;
RA   Barnett M.J., Fisher R.F., Jones T., Komp C., Abola A.P., Barloy-Hubler F.,
RA   Bowser L., Capela D., Galibert F., Gouzy J., Gurjal M., Hong A., Huizar L.,
RA   Hyman R.W., Kahn D., Kahn M.L., Kalman S., Keating D.H., Palm C.,
RA   Peck M.C., Surzycki R., Wells D.H., Yeh K.-C., Davis R.W., Federspiel N.A.,
RA   Long S.R.;
RT   "Nucleotide sequence and predicted functions of the entire Sinorhizobium
RT   meliloti pSymA megaplasmid.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9883-9888(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + L-arginine = L-citrulline + NH4(+);
CC         Xref=Rhea:RHEA:19597, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:32682, ChEBI:CHEBI:57743; EC=3.5.3.6;
CC   -!- PATHWAY: Amino-acid degradation; L-arginine degradation via ADI
CC       pathway; carbamoyl phosphate from L-arginine: step 1/2.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the arginine deiminase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE006469; AAK65573.1; -; Genomic_DNA.
DR   PIR; C95376; C95376.
DR   RefSeq; NP_436161.1; NC_003037.1.
DR   RefSeq; WP_010967881.1; NC_003037.1.
DR   AlphaFoldDB; Q92YG5; -.
DR   SMR; Q92YG5; -.
DR   EnsemblBacteria; AAK65573; AAK65573; SMa1670.
DR   GeneID; 61599673; -.
DR   KEGG; sme:SMa1670; -.
DR   PATRIC; fig|266834.11.peg.948; -.
DR   HOGENOM; CLU_052662_0_0_5; -.
DR   OMA; SAWIYDG; -.
DR   UniPathway; UPA00254; UER00364.
DR   Proteomes; UP000001976; Plasmid pSymA.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016990; F:arginine deiminase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019547; P:arginine catabolic process to ornithine; IEA:UniProtKB-UniPathway.
DR   GO; GO:0018101; P:protein citrullination; IEA:GOC.
DR   HAMAP; MF_00242; Arg_deiminase; 1.
DR   InterPro; IPR003876; Arg_deiminase.
DR   PIRSF; PIRSF006356; Arg_deiminase; 1.
DR   PRINTS; PR01466; ARGDEIMINASE.
DR   TIGRFAMs; TIGR01078; arcA; 1.
PE   3: Inferred from homology;
KW   Arginine metabolism; Cytoplasm; Hydrolase; Plasmid; Reference proteome.
FT   CHAIN           1..419
FT                   /note="Arginine deiminase 2"
FT                   /id="PRO_0000182231"
FT   ACT_SITE        407
FT                   /note="Amidino-cysteine intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   419 AA;  46510 MW;  C5240E0F9850B206 CRC64;
     MSSKSSTQHT FGVHSEVGQL RKVMVCAPGR AHQRLTPSNC DALLFDDVLW VDNARRDHFD
     FMTKMRDRGV EVVEMHNLLA QTVAIPEARK WILDNQVVPN QVGLELLDEI RSYLEGLPDR
     ELAETLIGGL STHEFPETHG GEMLELIRDA AGVAEYLLPP LPNTLYTRDT TCWIYGGVTL
     NPLYWPARHE ETILATAIYK FHPDFVGKVN VWWGEPTTDW GLATLEGGDV MPIGKGNVLI
     GMSERTSRQA ISQLAATLFE KGAAQRVIVA AMPKLRAAMH LDTVFTFADR DCVLIYPDIV
     NEIEAFSYRP GEKPGSLELH KDRGSFVETV RDALGLKEMR VVETGGNAYV RERTQWDSGA
     NLVCLSPGVV LAYDRNTYTN TLLRKAGVEV ITITGAELGR GRGGGHCMTC PIIRDAVDY