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KSL1_ISORU
ID   KSL1_ISORU              Reviewed;         626 AA.
AC   A0A1W6QDI7; A0A1X9ISN1;
DT   07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2017, sequence version 1.
DT   03-AUG-2022, entry version 19.
DE   RecName: Full=Miltiradiene synthase KSL1, chloroplastic {ECO:0000303|PubMed:28445526};
DE            EC=4.2.3.131 {ECO:0000269|PubMed:28381502, ECO:0000269|PubMed:28445526};
DE   AltName: Full=Kaurene synthase 1 {ECO:0000303|PubMed:28381502};
DE            Short=IrKSL1 {ECO:0000303|PubMed:28381502};
DE   AltName: Full=Terpene synthase 4 {ECO:0000303|PubMed:28445526};
DE            Short=IrTPS4 {ECO:0000303|PubMed:28445526};
DE   Flags: Precursor;
GN   Name=KSL1 {ECO:0000303|PubMed:28381502};
GN   Synonyms=TPS4 {ECO:0000303|PubMed:28445526};
OS   Isodon rubescens (Rabdosia rubescens).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Lamiales; Lamiaceae; Nepetoideae; Ocimeae; Isodoninae;
OC   Isodon.
OX   NCBI_TaxID=587669;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, PATHWAY, CATALYTIC
RP   ACTIVITY, AND TISSUE SPECIFICITY.
RX   PubMed=28381502; DOI=10.1104/pp.17.00202;
RA   Jin B., Cui G., Guo J., Tang J., Duan L., Lin H., Shen Y., Chen T.,
RA   Zhang H., Huang L.;
RT   "Functional diversification of kaurene synthase-like genes in Isodon
RT   rubescens.";
RL   Plant Physiol. 174:943-955(2017).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, PATHWAY, CATALYTIC
RP   ACTIVITY, AND TISSUE SPECIFICITY.
RX   PubMed=28445526; DOI=10.1371/journal.pone.0176507;
RA   Pelot K.A., Hagelthorn L.M., Addison J.B., Zerbe P.;
RT   "Biosynthesis of the oxygenated diterpene nezukol in the medicinal plant
RT   Isodon rubescens is catalyzed by a pair of diterpene synthases.";
RL   PLoS ONE 12:e0176507-e0176507(2017).
CC   -!- FUNCTION: Involved in the biosynthesis of ent-kaurene diterpenoids
CC       natural products such as oridonin, miltiradiene, eriocalyxin B and
CC       nezukol, known to exhibit antitumor, anti-inflammatory and
CC       antibacterial activities (PubMed:28381502, PubMed:28445526). Catalyzes
CC       the conversion of (+)-copalyl diphosphate ((+)-CPP) to miltiradiene
CC       (PubMed:28381502, PubMed:28445526). {ECO:0000269|PubMed:28381502,
CC       ECO:0000269|PubMed:28445526}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(+)-copalyl diphosphate = diphosphate + miltiradiene;
CC         Xref=Rhea:RHEA:33983, ChEBI:CHEBI:33019, ChEBI:CHEBI:58635,
CC         ChEBI:CHEBI:65037; EC=4.2.3.131;
CC         Evidence={ECO:0000269|PubMed:28381502, ECO:0000269|PubMed:28445526};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33984;
CC         Evidence={ECO:0000269|PubMed:28381502, ECO:0000269|PubMed:28445526};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:Q40577};
CC       Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000250|UniProtKB:Q40577};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC       {ECO:0000269|PubMed:28381502, ECO:0000269|PubMed:28445526}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=A0A1W6QDI7-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=A0A1W6QDI7-2; Sequence=VSP_060991, VSP_060992;
CC   -!- TISSUE SPECIFICITY: Highly expressed in roots, and, at low levels, in
CC       stems and leaves. {ECO:0000269|PubMed:28381502,
CC       ECO:0000269|PubMed:28445526}.
CC   -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC       the catalytic activity, presumably through binding to Mg(2+).
CC       {ECO:0000305}.
CC   -!- MISCELLANEOUS: Abietane diterpenoids (e.g. miltiradiene, abietatriene
CC       and ferruginol) accumulate specifically in the periderm of roots
CC       (PubMed:28381502). The ent-kaurene diterpenoid oridonin, main
CC       constituent of Isodon rubescens, accumulates in leaves
CC       (PubMed:28381502). {ECO:0000269|PubMed:28381502}.
CC   -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
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DR   EMBL; KU180504; APJ36376.1; -; mRNA.
DR   EMBL; KX831652; ARO38142.1; -; mRNA.
DR   AlphaFoldDB; A0A1W6QDI7; -.
DR   SMR; A0A1W6QDI7; -.
DR   BRENDA; 4.2.3.131; 15342.
DR   UniPathway; UPA00213; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0062205; F:miltiradiene synthase activity; IDA:UniProtKB.
DR   GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR   GO; GO:1901946; P:miltiradiene biosynthetic process; IDA:UniProtKB.
DR   GO; GO:0016114; P:terpenoid biosynthetic process; IDA:UniProtKB.
DR   Gene3D; 1.10.600.10; -; 1.
DR   Gene3D; 1.50.10.130; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR001906; Terpene_synth_N.
DR   InterPro; IPR036965; Terpene_synth_N_sf.
DR   InterPro; IPR005630; Terpene_synthase_metal-bd.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   Pfam; PF01397; Terpene_synth; 1.
DR   Pfam; PF03936; Terpene_synth_C; 1.
DR   SUPFAM; SSF48239; SSF48239; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Chloroplast; Lyase; Magnesium; Metal-binding;
KW   Plastid; Transit peptide.
FT   TRANSIT         1..51
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           52..626
FT                   /note="Miltiradiene synthase KSL1, chloroplastic"
FT                   /id="PRO_0000452385"
FT   MOTIF           329..333
FT                   /note="DDXXD motif"
FT                   /evidence="ECO:0000305"
FT   BINDING         329
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q40577"
FT   BINDING         329
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q40577"
FT   BINDING         333
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q40577"
FT   BINDING         333
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q40577"
FT   BINDING         473
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250|UniProtKB:Q40577"
FT   BINDING         481
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250|UniProtKB:Q40577"
FT   VAR_SEQ         592..597
FT                   /note="TELCQQ -> QSCVSN (in isoform 2)"
FT                   /id="VSP_060991"
FT   VAR_SEQ         598..626
FT                   /note="Missing (in isoform 2)"
FT                   /id="VSP_060992"
FT   CONFLICT        151
FT                   /note="H -> R (in Ref. 1; APJ36376)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        515
FT                   /note="L -> M (in Ref. 1; APJ36376)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   626 AA;  72049 MW;  9E49D6C54106B7B0 CRC64;
     MSLAFNLRVI PFSGHTIQSR RGLFPVHESP MITTKPFAAV KCSLTTSTDL MGKIKEKFNG
     KVHTSLPAIT THSADTPSNL CIIDTLQRLG VDRYFQSEID SILDDTYRLW QLKKEDIFSD
     ITTHAMAFRL LRVKGYQVSS EELAPYADQE HVNLQEIDVP TVIELYRAAQ ERVTEEDSTL
     KKLYVWTSTF LKQQLLTDAI PDKKLHEQVD YYLKNYHGIL DRMGVRRSLD LYDVGHYKTL
     KAADGFSNLC NEDFLAFARQ DFNISQAQHQ KELQQLQRWY SDCRLDTLKF GRDVVRVSNF
     LTSAMSGDPE LSDVRLAFAK HIVLVTRIDD FFDHGGSKEE SYKILELVKE WKEKPAGEYG
     SEEVEILFTA VYNTVNELAE MAHIEQGRSV KDLLIKLWVE ILSMFKIELD TWSDDTALTL
     DEYLSSSWVS IGCRICILIS MQFLGVKLTD EMLLSEECTD LCRHVSMVDR LLNDVQTFEK
     ERKENTGNSV SLLLAAHKDE RAINEEEAIT KAKDLAEYNR RKLMQIVYKT GTIFPRKCKD
     MFLKVCRIGC YLYSSGDEFT TPQQMMEDMK SLVYEPLTIH PPEANNVVGK KTELCQQLVK
     PYVCHTFCKK YTVSRPSNVM MTCYID
 
 
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