KSL2_ORYSJ
ID KSL2_ORYSJ Reviewed; 937 AA.
AC Q0JA81; A4KAG6; Q7XLD9;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 2.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Ent-kaurene synthase-like 2;
DE Short=OsKSL2;
DE EC=4.2.3.-;
GN Name=KSL2; OrderedLocusNames=Os04g0612000, LOC_Os04g52240;
GN ORFNames=OSJNBa0070C17.9;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 92-606.
RX PubMed=17141283; DOI=10.1016/j.phytochem.2006.10.016;
RA Xu M., Wilderman P.R., Morrone D., Xu J., Roy A., Margis-Pinheiro M.,
RA Upadhyaya N.M., Coates R.M., Peters R.J.;
RT "Functional characterization of the rice kaurene synthase-like gene
RT family.";
RL Phytochemistry 68:312-326(2007).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000250};
CC -!- TISSUE SPECIFICITY: Expressed in roots.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF15756.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAE05202.3; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL731610; CAE05202.3; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008210; BAF15756.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014960; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; DQ823350; ABH10731.1; -; mRNA.
DR AlphaFoldDB; Q0JA81; -.
DR SMR; Q0JA81; -.
DR STRING; 4530.OS04T0612000-00; -.
DR PRIDE; Q0JA81; -.
DR eggNOG; ENOG502QVGX; Eukaryota.
DR HOGENOM; CLU_003125_2_0_1; -.
DR InParanoid; Q0JA81; -.
DR PlantReactome; R-OSA-1119328; Oleoresin sesquiterpene volatiles biosynthesis.
DR PlantReactome; R-OSA-1119348; Ent-kaurene biosynthesis.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000059680; Chromosome 4.
DR GO; GO:0000287; F:magnesium ion binding; IBA:GO_Central.
DR GO; GO:0010333; F:terpene synthase activity; IBA:GO_Central.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IBA:GO_Central.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SUPFAM; SSF48239; SSF48239; 2.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 2: Evidence at transcript level;
KW Lyase; Magnesium; Metal-binding; Plant defense; Reference proteome.
FT CHAIN 1..937
FT /note="Ent-kaurene synthase-like 2"
FT /id="PRO_0000372313"
FT MOTIF 467..471
FT /note="DDXXD motif"
FT BINDING 467
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 467
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 471
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 471
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 611
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 615
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 619
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
SQ SEQUENCE 937 AA; 102596 MW; CE7F9ECD3BAD0714 CRC64;
MVPALRRGGG GPRFPQCVAW IQRNQRGDGS WRHAAAAHQQ LGSSPEIVTE RDLSSTLACV
LALARWDAGS EHVRRGLQFI GRNMSVAMDD QTAAPASGSV VSFAAMLRMA MEMGLEVPAV
SQADVRDRDA GVICHGGRTE YTAYVSEGLG NIQNWNEVMK FQRKNGSLFN SPYTTAAALV
HNYDAKALQY LDMLLDKFGS AVPAAYPANI QSQLYMVDVL EKMGISRHFV GEIKSILDMT
YSCWKQRDEE IVLDMQTCGM AFRMLRMNGY DVSSDELSHF SEPSSFHNSL QGYLNDTRSL
LELHKASKVS IAEKEVILDN IGSWTGCLLK EQLLSSAMKR NPLSEEVEYA LEFPFYTILD
RLDHKRNIEH FDITSSQMLE TAYLPCHSNE EIMALGVRDF SSSQFIFQEE LQQLNSWVKE
SRLDQLQFAR QKLDYFYFSA AATIFTPELS DVRILWAKNG VLTTVVDDFF DVGGSKEELE
NLVALVEKWD KNDKTEYYSE QVEIVFSAIY TSTNQLGSMA SVVQGRDVTK HLVEIWQELL
RSMMTEVEWR QSRYVPTAEE YMENAVVTFA LGPVVLPALY LVGPKMPDSV IRSQECSELF
RLMSKCGRLL NDVQSYEREG SQGKLNSVSL LALHSGGSVS MEEAVKQIQR PIEKCRRELL
KLVVSRGGAV PRPCRELFWS MCKVCHFFYS GGDGFSSPTA KAGALDAIPI CFVECDEKKK
NEEERIVIFW AEKKEKEKEK ERVNNKKMVL LLFTWVTGDD GGGAAEVAGA TGGLGHLPVA
VEGVGGVGEH DPGVLGVKPG VDDAGGLGSN DGGVKPPMGG SGGGWSSGAN GVEAKGGVGV
GVVVGVEDDG GAFGGLTQYG ELEPSPVASA RLAASPSGFA GEGDEEGSCI GSTVLMKLSL
YSAFSSVFLL LRLIRALASA PSTTTTTTQS NAHQKPN
