ID   KSL3_ORYSJ              Reviewed;         739 AA.
AC   Q0JA83; A0A0N7KJP1; Q0WYX1; Q69DS8; Q7XLE2;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   11-MAY-2016, sequence version 2.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Ent-kaurene synthase-like 3;
DE            Short=OsKS3;
DE            Short=OsKSL3;
DE            EC=4.2.3.-;
DE   AltName: Full=Ent-kaurene synthase-like 2;
DE            Short=OsKS2;
GN   Name=KSL3; Synonyms=KS2; OrderedLocusNames=Os04g0611700, LOC_Os04g52210;
GN   ORFNames=OSJNBa0070C17.6;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND
RP   DEVELOPMENTAL STAGE.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15668792; DOI=10.1007/s00299-004-0896-6;
RA   Margis-Pinheiro M., Zhou X.-R., Zhu Q.-H., Dennis E.S., Upadhyaya N.M.;
RT   "Isolation and characterization of a Ds-tagged rice (Oryza sativa L.) GA-
RT   responsive dwarf mutant defective in an early step of the gibberellin
RT   biosynthesis pathway.";
RL   Plant Cell Rep. 23:819-833(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447439; DOI=10.1038/nature01183;
RA   Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA   Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA   Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA   Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA   Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA   Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA   Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA   Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA   Li J., Hong G., Xue Y., Han B.;
RT   "Sequence and analysis of rice chromosome 4.";
RL   Nature 420:316-320(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [5]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO SER-698.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 7-739 (ISOFORM 2).
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16861806; DOI=10.1271/bbb.60044;
RA   Kanno Y., Otomo K., Kenmoku H., Mitsuhashi W., Yamane H., Oikawa H.,
RA   Toshima H., Matsuoka M., Sassa T., Toyomasu T.;
RT   "Characterization of a rice gene family encoding type-A diterpene
RT   cyclases.";
RL   Biosci. Biotechnol. Biochem. 70:1702-1710(2006).
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000250};
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q0JA83-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q0JA83-2; Sequence=VSP_037140, VSP_037141;
CC   -!- TISSUE SPECIFICITY: Expressed in roots and stems.
CC       {ECO:0000269|PubMed:15668792}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in seeds from 2 to 6 days after
CC       imbibition. {ECO:0000269|PubMed:15668792}.
CC   -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC       the catalytic activity, presumably through binding to Mg(2+).
CC   -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAE05199.3; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AY347879; AAQ72562.1; -; mRNA.
DR   EMBL; AL731610; CAE05199.3; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP008210; BAF15754.1; -; Genomic_DNA.
DR   EMBL; AP014960; BAS90961.1; -; Genomic_DNA.
DR   EMBL; AB243669; BAE98301.1; -; mRNA.
DR   AlphaFoldDB; Q0JA83; -.
DR   SMR; Q0JA83; -.
DR   STRING; 4530.OS04T0611700-00; -.
DR   PaxDb; Q0JA83; -.
DR   PRIDE; Q0JA83; -.
DR   EnsemblPlants; Os04t0611700-00; Os04t0611700-00; Os04g0611700. [Q0JA83-1]
DR   Gramene; Os04t0611700-00; Os04t0611700-00; Os04g0611700. [Q0JA83-1]
DR   eggNOG; ENOG502QVGX; Eukaryota.
DR   HOGENOM; CLU_003125_2_0_1; -.
DR   InParanoid; Q0JA83; -.
DR   OMA; YATMERT; -.
DR   Proteomes; UP000000763; Chromosome 4.
DR   Proteomes; UP000059680; Chromosome 4.
DR   Genevisible; Q0JA83; OS.
DR   GO; GO:0000287; F:magnesium ion binding; IBA:GO_Central.
DR   GO; GO:0010333; F:terpene synthase activity; IBA:GO_Central.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0016102; P:diterpenoid biosynthetic process; IBA:GO_Central.
DR   CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   Gene3D; 1.50.10.130; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR   InterPro; IPR001906; Terpene_synth_N.
DR   InterPro; IPR036965; Terpene_synth_N_sf.
DR   InterPro; IPR005630; Terpene_synthase_metal-bd.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   Pfam; PF01397; Terpene_synth; 1.
DR   Pfam; PF03936; Terpene_synth_C; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Lyase; Magnesium; Metal-binding; Plant defense;
KW   Reference proteome.
FT   CHAIN           1..739
FT                   /note="Ent-kaurene synthase-like 3"
FT                   /id="PRO_0000372314"
FT   MOTIF           475..479
FT                   /note="DDXXD motif"
FT   BINDING         475
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         475
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         479
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         479
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         619
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
FT   BINDING         623
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
FT   BINDING         627
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         417..441
FT                   /note="DELERIDSWVKENRLHELKFARQKS -> ALSNRSRSCSFGCQKFQYYSIYL
FT                   PG (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:16861806"
FT                   /id="VSP_037140"
FT   VAR_SEQ         442..739
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:16861806"
FT                   /id="VSP_037141"
FT   CONFLICT        191
FT                   /note="V -> I (in Ref. 6; BAE98301)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        371
FT                   /note="T -> I (in Ref. 6; BAE98301)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        698
FT                   /note="S -> A (in Ref. 2; CAE05199 and 3; BAF15754)"
SQ   SEQUENCE   739 AA;  84125 MW;  3C3F13A0F61C90C0 CRC64;
     MFQLELVNVV MHQRKAIEDT MRKKKKQQLH KFEMLPSPYD TAWVAMVPLP GSSSQLPCFP
     QCVEWILQNQ QSNGSWDLNQ LDSITKDALL STLACVLALR RGLLFIGRNF SIAMDEQLAA
     PIGFNITFPG MLSSVIEMGL EVPIGQTDVE RVLHLQETEL KREYEENYRG RNTYMAYVSE
     GLGNAQDWNE VMNFQRKNGS LFNSLSITAA VLVHNYDAKA HRYLNLLLNK FGTAVYTKNI
     HRQLSMLDAL ENMGISRHFD GEIKSILDMT YSCWLQRDEE VMLDITTCAM AFRILRMNGY
     DVSSDDLCHI AEVSDFHSSH QGYLSDTRTL LELYKASEVS VADNEFILDR IGSWSGRLLK
     EQLSSGALQR TSSIFEEVEH ALDCPFYATL DRLVHKRNIE HFAAMSYISY AQNNIPDELE
     RIDSWVKENR LHELKFARQK SAYFYLSAAG TVFDPEMSDA RIWWAINGVL TTVVDDFFDV
     GGSREELENL ISLVEMWDEH HKEELYSEQV EIVFFAIFNS VNQLGAKVSA VQGRDVTKHL
     IEIWLDLLRS MMTEVEWRIS NYVPTPEEYM ENAAMTFALG PIVLPALYLV GPKIPESVVR
     DSEYNELFRL MSTCGRLLND VQTYEREDGE GKVNSVSLLV IQSGGSVSIE EARREIMKPI
     ERCRRELLGL VLRRGSAVPG PCKELFWKMC KVCYFFYSRG DGFSSPTAKS AAVDAVIRDP
     LDLAAVVASQ EPIYIIPAS