KSL5_ISORU
ID KSL5_ISORU Reviewed; 794 AA.
AC A0A1Z3GC64;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2017, sequence version 1.
DT 03-AUG-2022, entry version 19.
DE RecName: Full=Ent-kaurene synthase 5, chloroplastic {ECO:0000303|PubMed:28381502};
DE Short=IrKSL5 {ECO:0000303|PubMed:28381502};
DE EC=4.2.3.19 {ECO:0000269|PubMed:28381502};
DE Flags: Precursor;
GN Name=KSL5 {ECO:0000303|PubMed:28381502};
OS Isodon rubescens (Rabdosia rubescens).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Lamiales; Lamiaceae; Nepetoideae; Ocimeae; Isodoninae;
OC Isodon.
OX NCBI_TaxID=587669;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY, AND
RP TISSUE SPECIFICITY.
RX PubMed=28381502; DOI=10.1104/pp.17.00202;
RA Jin B., Cui G., Guo J., Tang J., Duan L., Lin H., Shen Y., Chen T.,
RA Zhang H., Huang L.;
RT "Functional diversification of kaurene synthase-like genes in Isodon
RT rubescens.";
RL Plant Physiol. 174:943-955(2017).
CC -!- FUNCTION: Involved in the biosynthesis of ent-kaurene diterpenoids
CC natural products such as oridonin, miltiradiene, eriocalyxin B and
CC nezukol, known to exhibit antitumor, anti-inflammatory and
CC antibacterial activities, and in the production of gibberellins
CC phytohormones (PubMed:28381502). Catalyzes the conversion of ent-
CC copalyl diphosphate (ent-CPP) to ent-kaurene (PubMed:28381502).
CC {ECO:0000269|PubMed:28381502}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ent-copalyl diphosphate = diphosphate + ent-kaur-16-ene;
CC Xref=Rhea:RHEA:22220, ChEBI:CHEBI:15415, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58553; EC=4.2.3.19;
CC Evidence={ECO:0000269|PubMed:28381502};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22221;
CC Evidence={ECO:0000269|PubMed:28381502};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:Q40577};
CC Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000250|UniProtKB:Q40577};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC {ECO:0000269|PubMed:28381502}.
CC -!- PATHWAY: Plant hormone biosynthesis; gibberellin biosynthesis.
CC {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Ubiquitous expression in roots, stems, leaves and
CC flowers. {ECO:0000269|PubMed:28381502}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC {ECO:0000305}.
CC -!- MISCELLANEOUS: Abietane diterpenoids (e.g. miltiradiene, abietatriene
CC and ferruginol) accumulate specifically in the periderm of roots
CC (PubMed:28381502). The ent-kaurene diterpenoid oridonin, main
CC constituent of Isodon rubescens, accumulates in leaves
CC (PubMed:28381502). {ECO:0000269|PubMed:28381502}.
CC -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
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DR EMBL; KX580634; ASC55317.1; -; mRNA.
DR AlphaFoldDB; A0A1Z3GC64; -.
DR SMR; A0A1Z3GC64; -.
DR UniPathway; UPA00213; -.
DR UniPathway; UPA00390; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0009899; F:ent-kaurene synthase activity; IDA:UniProtKB.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0033331; P:ent-kaurene metabolic process; IDA:UniProtKB.
DR GO; GO:0009686; P:gibberellin biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IDA:UniProtKB.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SUPFAM; SSF48239; SSF48239; 2.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Lyase; Magnesium; Metal-binding; Plastid; Transit peptide.
FT TRANSIT 1..28
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 29..794
FT /note="Ent-kaurene synthase 5, chloroplastic"
FT /id="PRO_0000452387"
FT MOTIF 543..547
FT /note="DDXXD motif"
FT /evidence="ECO:0000305"
FT BINDING 543
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 543
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 547
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 547
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 687
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 695
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
SQ SEQUENCE 794 AA; 90379 MW; 95026AA2CAAB6940 CRC64;
MSLLLSNSAL VGPKFRSSRI SHASASLDIG LQRATSPQNA SVATCFEETK GRIAKLFHKN
ELSVSTYDTA WVAMVPSPTS SEEPCFPACL NWLLENQCHD GSWARPHHHH MLKKDVLSST
LACILALKKW GVGEEQINRG LHFVELNFAS ATEKGQITPM GFDIIFPAML DNARGLSLNL
QLEPTTLNDL IYKRDLELKR CNQSNSAEKE VYWAHIAEGM GKLQDWESVM KYQRKNGSLF
NCPSTTAAAF TALRNSDCLN YLRLALEKFG SAVPAVYPLD IYSQLCTVDN LERLGISRYF
LTEIQSVLDE TYRSWLQGDE EIFMDASTCA LAFRTLRMNG YNVTSDPITK ILQECFSSSF
RGNMTDINTT LEIYRASELI LYPEERDLDQ HNLRLKTFLE QELSSNGFIQ SCQLGRNINA
EVNQAIEYPF YAIMDRMAKR KNIENYNIDN TRILKTSYRS PNFGNKDFLS LSVEDFNRCQ
VIHREELREL ERWVIENRLD ELKFARSKAA YCYFSAAATI FSPELSDARM SWAKNGVLTT
VVDDFFDVGG SVEELKNLIQ LVELWDVDVS TQCCSPNVQI IFSALKHTIC EIADKGFKLQ
GRSITDHIIS IWLDLLYSMM KETELGIDKS FPTMDEYMSN AYVSFALGPI VLPALYLVGP
KLSEEMVNHS EYHTLFKLMS TCGRLLNDIR GYERELKDGK ISAVSLYIMN NGGEITTEAA
ISEMRSWIER DRRELLRLVL EENKSVLPKA CKKLFWHMCT VVHLFYRKDD GFTSLDLHGV
VNAIINEPIV LNQF
