位置:首页 > 蛋白库 > KSL7_ORYSI
KSL7_ORYSI
ID   KSL7_ORYSI              Reviewed;         830 AA.
AC   Q00G37; A2X6A1;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 2.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Ent-cassa-12,15-diene synthase;
DE            EC=4.2.3.28;
DE   AltName: Full=Diterpene cyclase 1;
DE            Short=OsDTC1;
DE   AltName: Full=Ent-kaurene synthase-like 7;
DE            Short=OsKSL7;
GN   Name=KSL7; Synonyms=DTC1; ORFNames=OsI_007594;
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39946;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. IR24;
RX   PubMed=16956633; DOI=10.1016/j.phytochem.2006.08.009;
RA   Peters R.J.;
RT   "Uncovering the complex metabolic network underlying diterpenoid
RT   phytoalexin biosynthesis in rice and other cereal crop plants.";
RL   Phytochemistry 67:2307-2317(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 93-11;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [3]
RP   FUNCTION.
RX   PubMed=17141283; DOI=10.1016/j.phytochem.2006.10.016;
RA   Xu M., Wilderman P.R., Morrone D., Xu J., Roy A., Margis-Pinheiro M.,
RA   Upadhyaya N.M., Coates R.M., Peters R.J.;
RT   "Functional characterization of the rice kaurene synthase-like gene
RT   family.";
RL   Phytochemistry 68:312-326(2007).
CC   -!- FUNCTION: Involved in phytocassane phytoalexins biosynthesis. Catalyzes
CC       the conversion of ent-copalyl diphosphate to the phytoalexin precursor
CC       ent-cassa-12,15-diene. {ECO:0000269|PubMed:17141283}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ent-copalyl diphosphate = diphosphate + ent-cassa-12,15-diene;
CC         Xref=Rhea:RHEA:25532, ChEBI:CHEBI:33019, ChEBI:CHEBI:50060,
CC         ChEBI:CHEBI:58553; EC=4.2.3.28;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000250};
CC   -!- TISSUE SPECIFICITY: Expressed in roots and stems.
CC   -!- DEVELOPMENTAL STAGE: Expressed from 2 to 6 days after imbibition.
CC   -!- INDUCTION: By chitin oligosaccharide elicitor and UV irradiation.
CC   -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC       the catalytic activity, presumably through binding to Mg(2+).
CC   -!- MISCELLANEOUS: Ent-cassa-12,15-diene is a precursor of the phytoalexins
CC       phytocassanes A-E. Phytoalexins are diterpenoid secondary metabolites
CC       involved in the defense mechanism of the plant and produced in response
CC       to attack (by a pathogen, elicitor or UV irradiation).
CC   -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAY86361.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; DQ823354; ABH10734.1; -; mRNA.
DR   EMBL; CM000127; EAY86361.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; Q00G37; -.
DR   SMR; Q00G37; -.
DR   STRING; 39946.Q00G37; -.
DR   PRIDE; Q00G37; -.
DR   HOGENOM; CLU_003125_2_0_1; -.
DR   Proteomes; UP000007015; Chromosome 2.
DR   GO; GO:0034277; F:ent-cassa-12,15-diene synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   Gene3D; 1.50.10.130; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR   InterPro; IPR001906; Terpene_synth_N.
DR   InterPro; IPR036965; Terpene_synth_N_sf.
DR   InterPro; IPR005630; Terpene_synthase_metal-bd.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   Pfam; PF01397; Terpene_synth; 1.
DR   Pfam; PF03936; Terpene_synth_C; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   2: Evidence at transcript level;
KW   Lyase; Magnesium; Metal-binding; Plant defense; Reference proteome.
FT   CHAIN           1..830
FT                   /note="Ent-cassa-12,15-diene synthase"
FT                   /id="PRO_0000372321"
FT   REGION          1..50
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           577..581
FT                   /note="DDXXD motif"
FT   BINDING         577
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         577
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         581
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         581
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         721
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
FT   BINDING         729
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        829
FT                   /note="S -> L (in Ref. 1; ABH10734)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   830 AA;  92180 MW;  CE5C635B0DD9AE45 CRC64;
     MMLLGSPSSG GYGGKFAGAS PAGGTTTMAP SAKQPSSRAP PPGITGGRND LRILSPAAAA
     AAVGGLEMKK PEAEGIAESL QATHRKELEA SIRKQLQGVE LSPSPYDTAW VAMVPLRGSS
     HNPSFPQCVD WILENQWDDG SWSIDGSIST ANKDVLSSTL ACVLALNKWN VGREHIRRGL
     SFIGRNFSIA MDDQAVAPIG FGITFPAMLT LANGSGLEVP VRQNDIDSLN HLREMKIQRE
     AGNHSRGRKA YMAYLAEGFG NLLEWDEIMM FQRKNGSLFN CPSSTAGALA NYHDDKALQY
     LQSLVNKFDG VVPTLYPLNI YCQLSMVDAL ENMGISQYFA SEIKSILDMT YSSWLGRDEE
     IMLDVTTCAM AFRLLRMNGY DVSSDELSHV AGASGFRDSL QGYLNDRKSV LEVYKTSKHS
     ISENDLILDS IGSWSGSLLK EMLCSNGIQG TPGREEIEFA LKYPFYSTLE RLVHRKNIVL
     FDAKGSQMLK TECMPVHDSQ DFLALAVDDF CISQSNYQNE LNYLESWVKD NRLDQLHFAR
     QKITYCYLSG AATTFRPEMG YARTSWARTA WLTAVIDDLF DVGGLEQEQE NLLALMEKWE
     EPGEDEYYSE DVKIVFQALY NTVNEIGAKA SALQGHDVTK YLVDVWLHVV RCMKVEAEWQ
     RSQHLPTFEE YMESGMVSLG QGATVMSALF LIGEKLPEGV VELEEYDEMF RLMGTCGRLL
     NDIRGIEREE SDGKMTNGVS LLVHASGGSM SVDEAKTEVM KRIDASRRKL LSLVVGEQEG
     PIPRPCKQLF WKMCKILHLF YYQTDGFSSP KEMVSAVDAV IKEPLQLRSL
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024