KSL8_ORYSJ
ID KSL8_ORYSJ Reviewed; 822 AA.
AC Q6BDZ9; Q2R4G8;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Stemar-13-ene synthase;
DE EC=4.2.3.33;
DE AltName: Full=Diterpene cyclase 2;
DE Short=OsDTC2;
DE AltName: Full=Ent-kaurene synthase-like 8;
DE Short=OsKSL8;
DE AltName: Full=Stemarene synthase;
GN Name=KSL8; Synonyms=DTC2; OrderedLocusNames=Os11g0474800, LOC_Os11g28530;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, AND INDUCTION.
RX PubMed=15280039; DOI=10.1016/j.febslet.2004.07.002;
RA Nemoto T., Cho E.-M., Okada A., Okada K., Otomo K., Kanno Y., Toyomasu T.,
RA Mitsuhashi W., Sassa T., Minami E., Shibuya N., Nishiyama M., Nojiri H.,
RA Yamane H.;
RT "Stemar-13-ene synthase, a diterpene cyclase involved in the biosynthesis
RT of the phytoalexin oryzalexin S in rice.";
RL FEBS Lett. 571:182-186(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Catalyzes the conversion of syn-copalyl diphosphate to the
CC phytoalexin precursor stemarene. {ECO:0000269|PubMed:15280039}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9alpha-copalyl diphosphate = diphosphate + stemar-13-ene;
CC Xref=Rhea:RHEA:25552, ChEBI:CHEBI:33019, ChEBI:CHEBI:50069,
CC ChEBI:CHEBI:58622; EC=4.2.3.33;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000250};
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6BDZ9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6BDZ9-2; Sequence=VSP_037142;
CC -!- INDUCTION: By chitin oligosaccharide elicitor and UV irradiation.
CC {ECO:0000269|PubMed:15280039}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC -!- MISCELLANEOUS: Stemar-13-ene is a putative precursor of the phytoalexin
CC oryzalexin S. Phytoalexins are diterpenoid secondary metabolites
CC involved in the defense mechanism of the plant and produced in response
CC to attack (by a pathogen, elicitor or UV irradiation).
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
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DR EMBL; AB118056; BAD34478.1; -; mRNA.
DR EMBL; DP000010; ABA93676.1; -; Genomic_DNA.
DR EMBL; AP008217; BAF28250.1; -; Genomic_DNA.
DR EMBL; AP014967; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_015617512.1; XM_015762026.1. [Q6BDZ9-1]
DR RefSeq; XP_015617513.1; XM_015762027.1. [Q6BDZ9-1]
DR RefSeq; XP_015617514.1; XM_015762028.1. [Q6BDZ9-1]
DR RefSeq; XP_015617515.1; XM_015762029.1. [Q6BDZ9-1]
DR AlphaFoldDB; Q6BDZ9; -.
DR SMR; Q6BDZ9; -.
DR STRING; 4530.OS11T0474800-01; -.
DR PaxDb; Q6BDZ9; -.
DR PRIDE; Q6BDZ9; -.
DR GeneID; 4350502; -.
DR KEGG; osa:4350502; -.
DR eggNOG; ENOG502QVGX; Eukaryota.
DR HOGENOM; CLU_003125_2_3_1; -.
DR InParanoid; Q6BDZ9; -.
DR OrthoDB; 318477at2759; -.
DR BioCyc; MetaCyc:DTC2-MON; -.
DR BRENDA; 4.2.3.33; 4460.
DR PlantReactome; R-OSA-1119521; Oryzalexin S biosynthesis.
DR Proteomes; UP000000763; Chromosome 11.
DR Proteomes; UP000059680; Chromosome 11.
DR Genevisible; Q6BDZ9; OS.
DR GO; GO:0000287; F:magnesium ion binding; IBA:GO_Central.
DR GO; GO:0034278; F:stemar-13-ene synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0010333; F:terpene synthase activity; IBA:GO_Central.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IBA:GO_Central.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SUPFAM; SSF48239; SSF48239; 2.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Lyase; Magnesium; Metal-binding; Plant defense;
KW Reference proteome.
FT CHAIN 1..822
FT /note="Stemar-13-ene synthase"
FT /id="PRO_0000372322"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 553..557
FT /note="DDXXD motif"
FT BINDING 553
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 553
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 557
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 557
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 698
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 702
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 706
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT VAR_SEQ 39..40
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15280039"
FT /id="VSP_037142"
SQ SEQUENCE 822 AA; 90258 MW; 0C2B7FB50DE0819C CRC64;
MMLLSSSYSG GQFPGVSPLG TRPKRSTTVV PRPVVTRATA GGVRNNLEVV GNAGTLQGMD
IDELRVIVRK QLQGVELSPS SYDTAWVAMV PVQGSRQSPC FPQCVEWILQ NQQEDGSWGH
SAGPSGEVNK DILLSTLACV LALNIWNVGQ DHIRRGLSFI GRNFSVAIDG QCAAPVGFNI
TFSGMLRLAI GMGLKFPVME TDIDSIFRLR EVEFERDAGG TASARKAFMA YVSEGLGREQ
DWDHVMAYQR KNGSLFNSPS TTAASAIHSC NDRALDYLVS LTSKLGGPVP AIYPDKVYSQ
LCMVDTLEKM GISSDFACDI RDILDMTYSC WMQDEEEIML DMATCAKAFR LLRMHGYDVS
SEGMARFAER SSFDDSIHAY LNDTKPLLEL YKSSQVHFLE EDFILENIGS WSAKLLKQQL
SFNKISKSLM PEVEYALKYP FYATVEVLEH KGNIERFNVN GFQRLKSGYC GSGADKEILA
LAVNKFHYAQ SVYQQELRYL ESWVAEFRLD ELKFARVIPL QSLLSAVVPL FPCELSDARI
AWSQNAILTA VVDDLFDGGG SMEEMLNLVA LFDKWDDHGE IGFCSSNVEI MFNAVYNTTK
RIGAKAALVQ KRCVIDHIAE QWQVMVRAML TEAEWAAGKH IPATMGEYMS VAEPSFALGP
IVPVSAYLLG EELPEEAVRS PEYGRLLGLA SAVGRLLNDV MTYEKEMGTG KLNSVVLLQP
LAAGGAASRG GGGAPAPAPA SVEAARAEVR RAIQASWRDL HGLVFGSGGG SSSSIIPRPC
REVFWHTGKV ASVFYQEGDG YARKAMRSMA NAVILEPLHL QE
