ID   KSTR2_MYCS2             Reviewed;         205 AA.
AC   A0R4Z6; I7G9M3;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=HTH-type transcriptional repressor KstR2;
GN   Name=kstR2; OrderedLocusNames=MSMEG_6009, MSMEI_5849;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   FUNCTION AS A TRANSCRIPTIONAL REPRESSOR.
RX   PubMed=20167624; DOI=10.1099/mic.0.034538-0;
RA   Kendall S.L., Burgess P., Balhana R., Withers M., Ten Bokum A., Lott J.S.,
RA   Gao C., Uhia-Castro I., Stoker N.G.;
RT   "Cholesterol utilization in mycobacteria is controlled by two TetR-type
RT   transcriptional regulators: kstR and kstR2.";
RL   Microbiology 156:1362-1371(2010).
CC   -!- FUNCTION: Controls the expression of a small regulon that may play a
CC       role in the utilization of cholesterol. {ECO:0000269|PubMed:20167624}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
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DR   EMBL; CP000480; ABK75778.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP42282.1; -; Genomic_DNA.
DR   RefSeq; WP_003897410.1; NZ_SIJM01000017.1.
DR   RefSeq; YP_890234.1; NC_008596.1.
DR   AlphaFoldDB; A0R4Z6; -.
DR   SMR; A0R4Z6; -.
DR   STRING; 246196.MSMEI_5849; -.
DR   EnsemblBacteria; ABK75778; ABK75778; MSMEG_6009.
DR   EnsemblBacteria; AFP42282; AFP42282; MSMEI_5849.
DR   GeneID; 66737294; -.
DR   KEGG; msg:MSMEI_5849; -.
DR   KEGG; msm:MSMEG_6009; -.
DR   PATRIC; fig|246196.19.peg.5846; -.
DR   eggNOG; COG1309; Bacteria.
DR   OMA; ARQYLSM; -.
DR   OrthoDB; 1437290at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IMP:UniProtKB.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001647; HTH_TetR.
DR   InterPro; IPR041490; KstR2_TetR_C.
DR   InterPro; IPR036271; Tet_transcr_reg_TetR-rel_C_sf.
DR   Pfam; PF17932; TetR_C_24; 1.
DR   Pfam; PF00440; TetR_N; 1.
DR   PRINTS; PR00455; HTHTETR.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF48498; SSF48498; 1.
DR   PROSITE; PS50977; HTH_TETR_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..205
FT                   /note="HTH-type transcriptional repressor KstR2"
FT                   /id="PRO_0000405032"
FT   DOMAIN          10..70
FT                   /note="HTH tetR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   DNA_BIND        33..52
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
SQ   SEQUENCE   205 AA;  23306 MW;  8300989F65E1868E CRC64;
     MAPDTPSQPA SRRDELLQLA ATMFADRGLK ATTVRDIADS AGILSGSLYH HFKSKEQMVE
     EVLRDFLDWL FGRYQQILDT ATSPLEKLTG LFMASFEAIE HRHAQVVIYQ DEAKRLSDLP
     QFDFVETRNK EQRKMWVDIL QEGVADGSFR PDLDVDLVYR FIRDTTWVSV RWYKPGGPLS
     AEQVGQQYLA IVLGGITQSQ GDKHA