ID   KSTR2_RHOJR             Reviewed;         204 AA.
AC   Q0S7V2;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   25-MAY-2022, entry version 112.
DE   RecName: Full=HTH-type transcriptional repressor KstR2;
GN   Name=kstR2; OrderedLocusNames=RHA1_ro04598;
OS   Rhodococcus jostii (strain RHA1).
OC   Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Rhodococcus.
OX   NCBI_TaxID=101510;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RHA1;
RX   PubMed=17030794; DOI=10.1073/pnas.0607048103;
RA   McLeod M.P., Warren R.L., Hsiao W.W.L., Araki N., Myhre M., Fernandes C.,
RA   Miyazawa D., Wong W., Lillquist A.L., Wang D., Dosanjh M., Hara H.,
RA   Petrescu A., Morin R.D., Yang G., Stott J.M., Schein J.E., Shin H.,
RA   Smailus D., Siddiqui A.S., Marra M.A., Jones S.J.M., Holt R.,
RA   Brinkman F.S.L., Miyauchi K., Fukuda M., Davies J.E., Mohn W.W.,
RA   Eltis L.D.;
RT   "The complete genome of Rhodococcus sp. RHA1 provides insights into a
RT   catabolic powerhouse.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15582-15587(2006).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS), AND SUBUNIT.
RC   STRAIN=RHA1;
RG   Midwest center for structural genomics (MCSG);
RT   "Crystal structure of probable transcriptional regulatory protein
RT   rha5900.";
RL   Submitted (FEB-2009) to the PDB data bank.
CC   -!- FUNCTION: Controls the expression of a small regulon that may play a
CC       role in the utilization of cholesterol. {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|Ref.2}.
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DR   EMBL; CP000431; ABG96384.1; -; Genomic_DNA.
DR   RefSeq; WP_009477673.1; NC_008268.1.
DR   PDB; 2IBD; X-ray; 1.50 A; A/B=1-204.
DR   PDBsum; 2IBD; -.
DR   AlphaFoldDB; Q0S7V2; -.
DR   SMR; Q0S7V2; -.
DR   STRING; 101510.RHA1_ro04598; -.
DR   EnsemblBacteria; ABG96384; ABG96384; RHA1_ro04598.
DR   KEGG; rha:RHA1_ro04598; -.
DR   eggNOG; COG1309; Bacteria.
DR   HOGENOM; CLU_069356_12_4_11; -.
DR   OMA; ARQYLSM; -.
DR   EvolutionaryTrace; Q0S7V2; -.
DR   Proteomes; UP000008710; Chromosome.
DR   GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001647; HTH_TetR.
DR   InterPro; IPR041490; KstR2_TetR_C.
DR   InterPro; IPR036271; Tet_transcr_reg_TetR-rel_C_sf.
DR   Pfam; PF17932; TetR_C_24; 1.
DR   Pfam; PF00440; TetR_N; 1.
DR   PRINTS; PR00455; HTHTETR.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF48498; SSF48498; 1.
DR   PROSITE; PS50977; HTH_TETR_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..204
FT                   /note="HTH-type transcriptional repressor KstR2"
FT                   /id="PRO_0000405033"
FT   DOMAIN          13..73
FT                   /note="HTH tetR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   DNA_BIND        36..55
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   HELIX           12..30
FT                   /evidence="ECO:0007829|PDB:2IBD"
FT   HELIX           37..43
FT                   /evidence="ECO:0007829|PDB:2IBD"
FT   HELIX           48..54
FT                   /evidence="ECO:0007829|PDB:2IBD"
FT   HELIX           58..82
FT                   /evidence="ECO:0007829|PDB:2IBD"
FT   HELIX           87..104
FT                   /evidence="ECO:0007829|PDB:2IBD"
FT   HELIX           106..114
FT                   /evidence="ECO:0007829|PDB:2IBD"
FT   HELIX           117..119
FT                   /evidence="ECO:0007829|PDB:2IBD"
FT   HELIX           123..125
FT                   /evidence="ECO:0007829|PDB:2IBD"
FT   HELIX           126..148
FT                   /evidence="ECO:0007829|PDB:2IBD"
FT   HELIX           158..168
FT                   /evidence="ECO:0007829|PDB:2IBD"
FT   HELIX           171..174
FT                   /evidence="ECO:0007829|PDB:2IBD"
FT   STRAND          180..182
FT                   /evidence="ECO:0007829|PDB:2IBD"
FT   HELIX           184..197
FT                   /evidence="ECO:0007829|PDB:2IBD"
SQ   SEQUENCE   204 AA;  22550 MW;  6C9FD9985640EEFA CRC64;
     MTPPPADDTS GKSGRRTELL DIAATLFAER GLRATTVRDI ADAAGILSGS LYHHFDSKES
     MVDEILRGFL DDLFGKYREI VASGLDSRAT LEALVTTSYE AIDASHSAVA IYQDEVKHLV
     ANERFTYLSE LNTEFRELWM GVLEAGVKDG SFRSDIDVEL AFRFLRDTAW VAVRWYRPGG
     SVTVDTVAKQ YLSIVLDGLA SPHN