ARCA_ENTFA
ID ARCA_ENTFA Reviewed; 408 AA.
AC Q93K67; Q7C3W8;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Arginine deiminase {ECO:0000255|HAMAP-Rule:MF_00242};
DE Short=ADI {ECO:0000255|HAMAP-Rule:MF_00242};
DE EC=3.5.3.6 {ECO:0000255|HAMAP-Rule:MF_00242};
DE AltName: Full=Arginine dihydrolase {ECO:0000255|HAMAP-Rule:MF_00242};
DE Short=AD {ECO:0000255|HAMAP-Rule:MF_00242};
GN Name=arcA {ECO:0000255|HAMAP-Rule:MF_00242}; OrderedLocusNames=EF_0104;
OS Enterococcus faecalis (strain ATCC 700802 / V583).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=226185;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 29212 / DSM 2570;
RX PubMed=12399499; DOI=10.1128/jb.184.22.6289-6300.2002;
RA Barcelona-Andres B., Marina A., Rubio V.;
RT "Gene structure, organization, expression, and potential regulatory
RT mechanisms of arginine catabolism in Enterococcus faecalis.";
RL J. Bacteriol. 184:6289-6300(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700802 / V583;
RX PubMed=12663927; DOI=10.1126/science.1080613;
RA Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R.,
RA Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H.,
RA Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C.,
RA DeBoy R.T., Durkin S.A., Kolonay J.F., Madupu R., Nelson W.C.,
RA Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M.,
RA Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M.;
RT "Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus
RT faecalis.";
RL Science 299:2071-2074(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + L-arginine = L-citrulline + NH4(+);
CC Xref=Rhea:RHEA:19597, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:32682, ChEBI:CHEBI:57743; EC=3.5.3.6;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00242};
CC -!- PATHWAY: Amino-acid degradation; L-arginine degradation via ADI
CC pathway; carbamoyl phosphate from L-arginine: step 1/2.
CC {ECO:0000255|HAMAP-Rule:MF_00242}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00242}.
CC -!- SIMILARITY: Belongs to the arginine deiminase family.
CC {ECO:0000255|HAMAP-Rule:MF_00242}.
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DR EMBL; AJ312276; CAC41341.1; -; Genomic_DNA.
DR EMBL; AE016830; AAO79979.1; -; Genomic_DNA.
DR RefSeq; NP_813907.1; NC_004668.1.
DR RefSeq; WP_002356129.1; NZ_KE136524.1.
DR AlphaFoldDB; Q93K67; -.
DR SMR; Q93K67; -.
DR STRING; 226185.EF_0104; -.
DR EnsemblBacteria; AAO79979; AAO79979; EF_0104.
DR GeneID; 60892653; -.
DR KEGG; efa:EF0104; -.
DR PATRIC; fig|226185.45.peg.156; -.
DR eggNOG; COG2235; Bacteria.
DR HOGENOM; CLU_052662_0_1_9; -.
DR OMA; ERATMHL; -.
DR UniPathway; UPA00254; UER00364.
DR Proteomes; UP000001415; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016990; F:arginine deiminase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019547; P:arginine catabolic process to ornithine; IEA:UniProtKB-UniPathway.
DR GO; GO:0018101; P:protein citrullination; IEA:GOC.
DR HAMAP; MF_00242; Arg_deiminase; 1.
DR InterPro; IPR003876; Arg_deiminase.
DR PIRSF; PIRSF006356; Arg_deiminase; 1.
DR PRINTS; PR01466; ARGDEIMINASE.
DR TIGRFAMs; TIGR01078; arcA; 1.
PE 3: Inferred from homology;
KW Arginine metabolism; Cytoplasm; Hydrolase; Reference proteome.
FT CHAIN 1..408
FT /note="Arginine deiminase"
FT /id="PRO_0000182211"
FT ACT_SITE 398
FT /note="Amidino-cysteine intermediate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00242"
SQ SEQUENCE 408 AA; 46735 MW; 42382220D96AD735 CRC64;
MSHPINVFSE IGKLKTVMLH RPGKELENLM PDYLERLLFD DIPFLEKAQA EHDAFAELLR
SKDIEVVYLE DLAAEALINE EVRRQFIDQF LEEANIRSES AKEKVRELML EIDDNEELIQ
KAIAGIQKQE LPKYEQEFLT DMVEADYPFI IDPMPNLYFT RDNFATMGHG ISLNHMYSVT
RQRETIFGQY IFDYHPRFAG KEVPRVYDRS ESTRIEGGDE LILSKEVVAI GISQRTDAAS
IEKIARNIFE QKLGFKNILA FDIGEHRKFM HLDTVFTMID YDKFTIHPEI EGGLVVYSIT
EKADGDIQIT KEKDTLDNIL CKYLHLDNVQ LIRCGAGNLT AAAREQWNDG SNTLAIAPGE
VVVYDRNTIT NKALEEAGVK LNYIPGSELV RGRGGPRCMS MPLYREDL