ID   ARCA_HALS3              Reviewed;         486 AA.
AC   B0R9X5; Q48294; Q7LY13;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=Arginine deiminase;
DE            Short=ADI;
DE            EC=3.5.3.6;
DE   AltName: Full=Arginine dihydrolase;
DE            Short=AD;
GN   Name=arcA; OrderedLocusNames=OE_5208R;
OS   Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1).
OG   Plasmid PHS3.
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Halobacteriaceae; Halobacterium.
OX   NCBI_TaxID=478009;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 376-385; 393-404 AND
RP   442-460, FUNCTION, PATHWAY, AND INDUCTION.
RC   STRAIN=R1 / S9 / L33;
RX   PubMed=8759859; DOI=10.1128/jb.178.16.4942-4947.1996;
RA   Ruepp A., Soppa J.;
RT   "Fermentative arginine degradation in Halobacterium salinarium (formerly
RT   Halobacterium halobium): genes, gene products, and transcripts of the
RT   arcRACB gene cluster.";
RL   J. Bacteriol. 178:4942-4947(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29341 / DSM 671 / R1; PLASMID=PHS3;
RX   PubMed=18313895; DOI=10.1016/j.ygeno.2008.01.001;
RA   Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K.,
RA   Ruepp A., Soppa J., Tittor J., Oesterhelt D.;
RT   "Evolution in the laboratory: the genome of Halobacterium salinarum strain
RT   R1 compared to that of strain NRC-1.";
RL   Genomics 91:335-346(2008).
CC   -!- FUNCTION: Involved in the arginine deiminase pathway of fermentative
CC       arginine utilization. {ECO:0000269|PubMed:8759859}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + L-arginine = L-citrulline + NH4(+);
CC         Xref=Rhea:RHEA:19597, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:32682, ChEBI:CHEBI:57743; EC=3.5.3.6;
CC   -!- PATHWAY: Amino-acid degradation; L-arginine degradation via ADI
CC       pathway; carbamoyl phosphate from L-arginine: step 1/2.
CC       {ECO:0000269|PubMed:8759859}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- INDUCTION: No induction in fermentatively grown cells.
CC       {ECO:0000269|PubMed:8759859}.
CC   -!- SIMILARITY: Belongs to the arginine deiminase family. {ECO:0000305}.
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DR   EMBL; X80931; CAA56904.1; -; Genomic_DNA.
DR   EMBL; AM774418; CAP15556.1; -; Genomic_DNA.
DR   PIR; T44863; T44863.
DR   RefSeq; WP_010904161.1; NC_010368.1.
DR   AlphaFoldDB; B0R9X5; -.
DR   SMR; B0R9X5; -.
DR   EnsemblBacteria; CAP15556; CAP15556; OE_5208R.
DR   GeneID; 5954932; -.
DR   GeneID; 62888216; -.
DR   KEGG; hsl:OE_5208R; -.
DR   HOGENOM; CLU_049514_0_0_2; -.
DR   OMA; MHLDTYF; -.
DR   PhylomeDB; B0R9X5; -.
DR   UniPathway; UPA00254; UER00364.
DR   Proteomes; UP000001321; Plasmid PHS3.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016990; F:arginine deiminase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019547; P:arginine catabolic process to ornithine; IEA:UniProtKB-UniPathway.
DR   GO; GO:0018101; P:protein citrullination; IEA:GOC.
PE   1: Evidence at protein level;
KW   Arginine metabolism; Cytoplasm; Direct protein sequencing; Hydrolase;
KW   Plasmid.
FT   CHAIN           1..486
FT                   /note="Arginine deiminase"
FT                   /id="PRO_0000428925"
FT   ACT_SITE        476
FT                   /note="Amidino-cysteine intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   486 AA;  54706 MW;  FE6E726E1B60F8A1 CRC64;
     MSSESPESVD DSTKVQATAE WDPLQAVRMH LPGAETFVGI MDPEPNLFLN DFSLVDAQQE
     HLSLSQDLEA ALPASNPIHY LHDDLANGNG MNALLSSRVN FDLSELGEDE QVNRRDKMWA
     RLHELSPHTQ IQAVLGNAEV IRHHSHSDEE VPGSNPDRWD TTSVQLEQPL TNMYFQRDQQ
     FVTQNGVVLC SMKEDTRKPE VDIARASWEA LDGDEFDVDI VADMSQVREH DVTEHVPERD
     DIQETEVLVE GGDFYPAGEF SLLGVSAKIP EGEAYPKHDI AEDDTEYVHR TTYAAGHRLL
     MDDAFGSEEV GLVRAPFEAA QAHKDDDNGE VEMDIMHLDT WFNFVDDDLV VAHKELVENT
     TLDVYARTHG GEKPYTLERP DVNFGEYLRE KGFEIVDVYD YVDPSHPDTD MALKAITNFL
     TVGPRKILPV RFSDDDDGVM KQFIDGIQED YDVTVIPDGE GRKIINLRAG YGAIHCMTTP
     LRRTPE