ID   ARCA_LENHI              Reviewed;         418 AA.
AC   Q8G999;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=Arginine deiminase {ECO:0000255|HAMAP-Rule:MF_00242};
DE            Short=ADI {ECO:0000255|HAMAP-Rule:MF_00242};
DE            EC=3.5.3.6 {ECO:0000255|HAMAP-Rule:MF_00242};
DE   AltName: Full=Arginine dihydrolase {ECO:0000255|HAMAP-Rule:MF_00242};
DE            Short=AD {ECO:0000255|HAMAP-Rule:MF_00242};
GN   Name=arcA {ECO:0000255|HAMAP-Rule:MF_00242};
OS   Lentilactobacillus hilgardii (Lactobacillus hilgardii).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lentilactobacillus.
OX   NCBI_TaxID=1588;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=X(1)B;
RX   PubMed=12490324; DOI=10.1016/s0378-1119(02)01083-1;
RA   Arena M.E., Manca de Nadra M.C., Munoz R.;
RT   "The arginine deiminase pathway in the wine lactic acid bacterium
RT   Lactobacillus hilgardii X1B: structural and functional study of the arcABC
RT   genes.";
RL   Gene 301:61-66(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + L-arginine = L-citrulline + NH4(+);
CC         Xref=Rhea:RHEA:19597, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:32682, ChEBI:CHEBI:57743; EC=3.5.3.6;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00242};
CC   -!- PATHWAY: Amino-acid degradation; L-arginine degradation via ADI
CC       pathway; carbamoyl phosphate from L-arginine: step 1/2.
CC       {ECO:0000255|HAMAP-Rule:MF_00242}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00242}.
CC   -!- SIMILARITY: Belongs to the arginine deiminase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00242}.
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DR   EMBL; AJ421514; CAD13391.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8G999; -.
DR   SMR; Q8G999; -.
DR   UniPathway; UPA00254; UER00364.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016990; F:arginine deiminase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019547; P:arginine catabolic process to ornithine; IEA:UniProtKB-UniPathway.
DR   GO; GO:0018101; P:protein citrullination; IEA:GOC.
DR   HAMAP; MF_00242; Arg_deiminase; 1.
DR   InterPro; IPR003876; Arg_deiminase.
DR   PIRSF; PIRSF006356; Arg_deiminase; 1.
DR   PRINTS; PR01466; ARGDEIMINASE.
DR   TIGRFAMs; TIGR01078; arcA; 1.
PE   3: Inferred from homology;
KW   Arginine metabolism; Cytoplasm; Hydrolase.
FT   CHAIN           1..418
FT                   /note="Arginine deiminase"
FT                   /id="PRO_0000182212"
FT   ACT_SITE        406
FT                   /note="Amidino-cysteine intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00242"
SQ   SEQUENCE   418 AA;  47173 MW;  C56AF3C17B859A29 CRC64;
     MEVTQMTSPI HNYSEIGKLK TVLLKRPGRE IENFTPDMMP RLLFDDIPYL PIAQKEHDYF
     ADTLRDNGVE VLYLEKLSAE ALDAGGEEVR NHFLEQMLTE SGYAAGSIHD ALKEYLGSMN
     NQDMVVKIME GVRKNELDFV PKDLVSCAEN QDYEFYMDPM PNLYFTRDPS ACIGDGLSIN
     HMTFVARQRE SLFNETIIKY HPRFANKGIH VWRDRNHTTR IEGGDELVLN NHVMAIGVSQ
     RTSADAIQDI AKNLFKDGQY DTVIAIKIPH NHAMMHLDTV FTMINYDQFT VHPGILGKGG
     QIDTWTITPG KNEGELNLQH DTDLKKVLKT ALNLDDLDLI PTGNGDPIIA GREQWNDGSN
     TLAIAPGVVV TYNRNYVSNE LLRKHGLKVL EVISSELSRG RGGPRCMSCP IVREDLKK