ID   KTI2_SOYBN              Reviewed;         204 AA.
AC   P25273;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Kunitz-type trypsin inhibitor KTI2;
DE   Flags: Precursor;
GN   Name=KTI2;
OS   Glycine max (Soybean) (Glycine hispida).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC   Glycine subgen. Soja.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Forrest;
RX   PubMed=2562561; DOI=10.2307/3869025;
RA   Jofuku K.D., Goldberg R.B.;
RT   "Kunitz trypsin inhibitor genes are differentially expressed during the
RT   soybean life cycle and in transformed tobacco plants.";
RL   Plant Cell 1:1079-1093(1989).
CC   -!- FUNCTION: Has probably no trypsin inhibitor activity. KTi2 is
CC       responsible for most of the Kunitz trypsin inhibitor activity and
CC       protein found in soybean seeds.
CC   -!- TISSUE SPECIFICITY: Seed, and at low levels in leaf, root, and stem.
CC   -!- DEVELOPMENTAL STAGE: Expressed during embryogenesis and in mature
CC       plant.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I3 (leguminous Kunitz-
CC       type inhibitor) family. {ECO:0000305}.
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DR   EMBL; S45035; AAB23483.1; -; Genomic_DNA.
DR   PIR; JQ1092; JQ1092.
DR   AlphaFoldDB; P25273; -.
DR   SMR; P25273; -.
DR   MEROPS; I03.001; -.
DR   PRIDE; P25273; -.
DR   InParanoid; P25273; -.
DR   Proteomes; UP000008827; Unplaced.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00178; STI; 1.
DR   InterPro; IPR011065; Kunitz_inhibitor_STI-like_sf.
DR   InterPro; IPR002160; Prot_inh_Kunz-lg.
DR   PANTHER; PTHR33107; PTHR33107; 1.
DR   Pfam; PF00197; Kunitz_legume; 1.
DR   PRINTS; PR00291; KUNITZINHBTR.
DR   SMART; SM00452; STI; 1.
DR   SUPFAM; SSF50386; SSF50386; 1.
DR   PROSITE; PS00283; SOYBEAN_KUNITZ; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Protease inhibitor; Reference proteome;
KW   Serine protease inhibitor; Signal.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..204
FT                   /note="Kunitz-type trypsin inhibitor KTI2"
FT                   /id="PRO_0000016892"
FT   DISULFID        65..112
FT                   /evidence="ECO:0000250"
FT   DISULFID        160..169
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   204 AA;  22800 MW;  E22D38BA565A99BE CRC64;
     MKSTIFFALF LVCAFTISYL PSATAQFVLD TDDDPLQNGG TYYMLPVMRG KSGGIEGNST
     GKEICPLTVV QSPNKHNKGI GLVFKSPLHA LFIAERYPLS IKFDSFAVIP LCGVMPTKWA
     IVEREGLQAV TLAARDTVDG WFNIERVSRE YNDYYKLVFC PQEAEDNKCE DIGIQIDNDG
     IRRLVLSKNK PLVVEFQKFR SSTA