ID   ARCA_MYCTU              Reviewed;         402 AA.
AC   P9WQ05; L0T5J1; O05585; P63551;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 36.
DE   RecName: Full=Arginine deiminase;
DE            Short=ADI;
DE            EC=3.5.3.6;
DE   AltName: Full=Arginine dihydrolase;
DE            Short=AD;
GN   Name=arcA; OrderedLocusNames=Rv1001; ORFNames=MTCI237.16;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
RX   PubMed=19099550; DOI=10.1186/1752-0509-2-109;
RA   Raman K., Yeturu K., Chandra N.;
RT   "targetTB: a target identification pipeline for Mycobacterium tuberculosis
RT   through an interactome, reactome and genome-scale structural analysis.";
RL   BMC Syst. Biol. 2:109-109(2008).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + L-arginine = L-citrulline + NH4(+);
CC         Xref=Rhea:RHEA:19597, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:32682, ChEBI:CHEBI:57743; EC=3.5.3.6;
CC   -!- PATHWAY: Amino-acid degradation; L-arginine degradation via ADI
CC       pathway; carbamoyl phosphate from L-arginine: step 1/2.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- MISCELLANEOUS: Was identified as a high-confidence drug target.
CC   -!- SIMILARITY: Belongs to the arginine deiminase family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP43751.1; -; Genomic_DNA.
DR   PIR; D70602; D70602.
DR   RefSeq; NP_215517.1; NC_000962.3.
DR   RefSeq; WP_003405169.1; NZ_NVQJ01000018.1.
DR   AlphaFoldDB; P9WQ05; -.
DR   SMR; P9WQ05; -.
DR   STRING; 83332.Rv1001; -.
DR   PaxDb; P9WQ05; -.
DR   GeneID; 45424973; -.
DR   GeneID; 888313; -.
DR   KEGG; mtu:Rv1001; -.
DR   TubercuList; Rv1001; -.
DR   eggNOG; COG2235; Bacteria.
DR   OMA; ERATMHL; -.
DR   PhylomeDB; P9WQ05; -.
DR   UniPathway; UPA00254; UER00364.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0016990; F:arginine deiminase activity; IBA:GO_Central.
DR   GO; GO:0019547; P:arginine catabolic process to ornithine; IEA:UniProtKB-UniPathway.
DR   GO; GO:0019546; P:arginine deiminase pathway; IBA:GO_Central.
DR   GO; GO:0018101; P:protein citrullination; IEA:GOC.
DR   HAMAP; MF_00242; Arg_deiminase; 1.
DR   InterPro; IPR003876; Arg_deiminase.
DR   PIRSF; PIRSF006356; Arg_deiminase; 1.
DR   PRINTS; PR01466; ARGDEIMINASE.
DR   TIGRFAMs; TIGR01078; arcA; 1.
PE   1: Evidence at protein level;
KW   Arginine metabolism; Cytoplasm; Hydrolase; Reference proteome.
FT   CHAIN           1..402
FT                   /note="Arginine deiminase"
FT                   /id="PRO_0000182223"
FT   ACT_SITE        392
FT                   /note="Amidino-cysteine intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   402 AA;  43089 MW;  16E5B4BEAA1745D2 CRC64;
     MGVELGSNSE VGALRVVILH RPGAELRRLT PRNTDQLLFD GLPWVSRAQD EHDEFAELLA
     SRGAEVLLLS DLLTEALHHS GAARMQGIAA AVDAPRLGLP LAQELSAYLR SLDPGRLAHV
     LTAGMTFNEL PSDTRTDVSL VLRMHHGGDF VIEPLPNLVF TRDSSIWIGP RVVIPSLALR
     ARVREASLTD LIYAHHPRFT GVRRAYESRT APVEGGDVLL LAPGVVAVGV GERTTPAGAE
     ALARSLFDDD LAHTVLAVPI AQQRAQMHLD TVCTMVDTDT MVMYANVVDT LEAFTIQRTP
     DGVTIGDAAP FAEAAAKAMG IDKLRVIHTG MDPVVAEREQ WDDGNNTLAL APGVVVAYER
     NVQTNARLQD AGIEVLTIAG SELGTGRGGP RCMSCPAARD PL