ID   KTI3_ARATH              Reviewed;         222 AA.
AC   Q9FX28;
DT   31-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=Kunitz trypsin inhibitor 3 {ECO:0000303|PubMed:30042779};
DE            Short=AtKTI3 {ECO:0000303|PubMed:30042779};
DE   Flags: Precursor;
GN   Name=KTI3 {ECO:0000303|PubMed:30042779};
GN   OrderedLocusNames=At1g73325 {ECO:0000312|Araport:AT1G73325};
GN   ORFNames=T9L24.46 {ECO:0000312|EMBL:AAG30988.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   INDUCTION BY SPIDER MITES, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=30042779; DOI=10.3389/fpls.2018.00986;
RA   Arnaiz A., Talavera-Mateo L., Gonzalez-Melendi P., Martinez M., Diaz I.,
RA   Santamaria M.E.;
RT   "Arabidopsis Kunitz trypsin inhibitors in defense against spider mites.";
RL   Front. Plant Sci. 9:986-986(2018).
CC   -!- FUNCTION: Exhibits Kunitz trypsin protease inhibitor activity.
CC       {ECO:0000250|UniProtKB:Q8RXD5}.
CC   -!- INDUCTION: Induced by infestation with spider mites.
CC       {ECO:0000269|PubMed:30042779}.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I3 (leguminous Kunitz-
CC       type inhibitor) family. {ECO:0000305}.
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DR   EMBL; AC012396; AAG30988.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE35443.1; -; Genomic_DNA.
DR   PIR; F96759; F96759.
DR   RefSeq; NP_565062.1; NM_105992.2.
DR   AlphaFoldDB; Q9FX28; -.
DR   SMR; Q9FX28; -.
DR   PaxDb; Q9FX28; -.
DR   PRIDE; Q9FX28; -.
DR   ProteomicsDB; 187823; -.
DR   EnsemblPlants; AT1G73325.1; AT1G73325.1; AT1G73325.
DR   GeneID; 843667; -.
DR   Gramene; AT1G73325.1; AT1G73325.1; AT1G73325.
DR   KEGG; ath:AT1G73325; -.
DR   Araport; AT1G73325; -.
DR   TAIR; locus:505006220; AT1G73325.
DR   eggNOG; ENOG502R58H; Eukaryota.
DR   HOGENOM; CLU_090145_1_1_1; -.
DR   InParanoid; Q9FX28; -.
DR   OMA; SKVWRID; -.
DR   OrthoDB; 1481039at2759; -.
DR   PhylomeDB; Q9FX28; -.
DR   PRO; PR:Q9FX28; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9FX28; baseline and differential.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00178; STI; 1.
DR   InterPro; IPR011065; Kunitz_inhibitor_STI-like_sf.
DR   InterPro; IPR002160; Prot_inh_Kunz-lg.
DR   PANTHER; PTHR33107; PTHR33107; 1.
DR   Pfam; PF00197; Kunitz_legume; 1.
DR   PRINTS; PR00291; KUNITZINHBTR.
DR   SMART; SM00452; STI; 1.
DR   SUPFAM; SSF50386; SSF50386; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Glycoprotein; Protease inhibitor; Reference proteome;
KW   Serine protease inhibitor; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..222
FT                   /note="Kunitz trypsin inhibitor 3"
FT                   /id="PRO_5014312886"
FT   CARBOHYD        65
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        175
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   DISULFID        72..119
FT                   /evidence="ECO:0000250|UniProtKB:P01070"
FT   DISULFID        165..173
FT                   /evidence="ECO:0000250|UniProtKB:P01070"
SQ   SEQUENCE   222 AA;  24309 MW;  1EA4944934395140 CRC64;
     MEKLTLSFIT LTVLSAIFTA ASAADATPSQ VVLDIAGHPV QSNVQYYIIP AKIGTGGGLI
     PSNRNLSTQD LCLNLDIVQS SSPFVSGLPV TFSPLNTKVK HVQLSASLNL EFDSTVWLCP
     DSKVWRIDHS VQLRKSFVSI GGQKGKGNSW FQIQEDGDAY KLMYCPISSI VACINVSLEI
     DDHGVRRLVL STDQSFVVKF QKAYDSNSNC NLKSNSRMFL FL