KTNB1_XENLA
ID KTNB1_XENLA Reviewed; 655 AA.
AC Q4V7Y7; O93320; Q6DE69;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Katanin p80 WD40 repeat-containing subunit B1 {ECO:0000255|HAMAP-Rule:MF_03022};
DE Short=Katanin p80 subunit B1 {ECO:0000255|HAMAP-Rule:MF_03022};
DE AltName: Full=p80 katanin {ECO:0000255|HAMAP-Rule:MF_03022};
GN Name=katnb1;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney, and Ovary;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 305-655, FUNCTION, INTERACTION WITH KATNA1,
RP AND SUBCELLULAR LOCATION.
RC TISSUE=Ovary;
RX PubMed=9658175; DOI=10.1091/mbc.9.7.1847;
RA McNally F.J., Thomas S.;
RT "Katanin is responsible for the M-phase microtubule-severing activity in
RT Xenopus eggs.";
RL Mol. Biol. Cell 9:1847-1861(1998).
CC -!- FUNCTION: Participates in a complex which severs microtubules in an
CC ATP-dependent manner. May act to target the enzymatic subunit of this
CC complex to sites of action such as the centrosome. Microtubule severing
CC may promote rapid reorganization of cellular microtubule arrays and the
CC release of microtubules from the centrosome following nucleation.
CC {ECO:0000255|HAMAP-Rule:MF_03022, ECO:0000269|PubMed:9658175}.
CC -!- SUBUNIT: Interacts with katna1. This interaction enhances the
CC microtubule binding and severing activity of katna1 and also targets
CC this activity to the centrosome. {ECO:0000255|HAMAP-Rule:MF_03022}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03022,
CC ECO:0000269|PubMed:9658175}. Cytoplasm, cytoskeleton, microtubule
CC organizing center, centrosome {ECO:0000255|HAMAP-Rule:MF_03022,
CC ECO:0000269|PubMed:9658175}. Cytoplasm, cytoskeleton, spindle pole
CC {ECO:0000255|HAMAP-Rule:MF_03022, ECO:0000269|PubMed:9658175}.
CC Cytoplasm, cytoskeleton {ECO:0000255|HAMAP-Rule:MF_03022,
CC ECO:0000269|PubMed:9658175}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000250|UniProtKB:Q9BVA0}. Note=Predominantly cytoplasmic.
CC Localized to the interphase centrosome and mitotic spindle poles.
CC -!- SIMILARITY: Belongs to the WD repeat KATNB1 family. {ECO:0000255|HAMAP-
CC Rule:MF_03022}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH77273.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305};
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DR EMBL; BC077273; AAH77273.1; ALT_SEQ; mRNA.
DR EMBL; BC097654; AAH97654.1; -; mRNA.
DR EMBL; AF056021; AAC25113.1; -; mRNA.
DR RefSeq; NP_001081754.1; NM_001088285.1.
DR AlphaFoldDB; Q4V7Y7; -.
DR SMR; Q4V7Y7; -.
DR BioGRID; 99367; 1.
DR DNASU; 398032; -.
DR GeneID; 398032; -.
DR KEGG; xla:398032; -.
DR CTD; 398032; -.
DR Xenbase; XB-GENE-998277; katnb1.L.
DR Proteomes; UP000186698; Chromosome 4L.
DR Bgee; 398032; Expressed in ovary and 19 other tissues.
DR GO; GO:0005813; C:centrosome; IEA:UniProtKB-UniRule.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0008352; C:katanin complex; IEA:InterPro.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005819; C:spindle; ISS:UniProtKB.
DR GO; GO:0000922; C:spindle pole; ISS:UniProtKB.
DR GO; GO:0008017; F:microtubule binding; IEA:UniProtKB-UniRule.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0051013; P:microtubule severing; IEA:UniProtKB-UniRule.
DR Gene3D; 2.130.10.10; -; 2.
DR HAMAP; MF_03022; Katanin_p80_B1; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR028021; Katanin_C-terminal.
DR InterPro; IPR026962; KTNB1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF13925; Katanin_con80; 1.
DR Pfam; PF00400; WD40; 5.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 3.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Cytoplasm; Cytoskeleton; Microtubule; Mitosis;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..655
FT /note="Katanin p80 WD40 repeat-containing subunit B1"
FT /id="PRO_0000270748"
FT REPEAT 18..58
FT /note="WD 1"
FT REPEAT 61..100
FT /note="WD 2"
FT REPEAT 103..142
FT /note="WD 3"
FT REPEAT 145..184
FT /note="WD 4"
FT REPEAT 187..226
FT /note="WD 5"
FT REPEAT 229..269
FT /note="WD 6"
FT REGION 338..397
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 411..431
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 350..370
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 412..430
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 402
FT /note="V -> I (in Ref. 2; AAC25113)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 655 AA; 71593 MW; EA24B2D6920509E3 CRC64;
MAAPSPTKTT WKLQEIVAHG CSVSSVVLGR SSGRLVATGG DDCRVHLWSV NKPNCIMSLT
GHTTPVESVR FNNSEELIVA GSQSGSLRIW DLEAAKILRT LMGHKANVSS LDFHPYGEFV
ASGSLDTNIK LWDVRRKGCV FRYKGHTQAV RCLRFSPDGK WLASASDDHS VKLWDLTAGK
MMAELSEHKG PVNIIEFHPN EYLLASGSAD RTVRFWDLEK FQLVGCTEGE TIPVRAILFS
NDGGCIFCGG KDSLRVYGWE PDQCFDTVPV GWGKVSDLAI CNNQLIGVSS AQSNISSFVV
DLTRVKMTGC APQGPVPAEI PISQPAPTGT SLRRIYERPS TTCSKPNRVS PTSDDEEKES
RAEIQNPEDY KEIFQPKNAI SRTPPRNSEP FPAPPEDDIS IVKEAVAPTP DVVTPATSNK
KNTEQLQRPP VAASTPIVCQ EPSPVPAPQS KPPVISAARN EPIGLKAADF LPAVKSSSPT
EVVDDEAVSQ IRKGHDTMCM VLTSRMRNLD TVRAVWSSGD IKTSIDSAVA INDLSVVVDL
LNIINQKASL WKLDLCMTVL PQIEKMLQSK YESYVQTGCI SLKLILQRFL PLITDILAAP
PSVGVDISRE ERLSKCKLCY KQLRILSPLV KSKASQSGRY GSAFRELHLL MSGLE
