KTR2_YEAST
ID KTR2_YEAST Reviewed; 425 AA.
AC P33550; D6VXC2;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Probable mannosyltransferase KTR2;
DE EC=2.4.1.-;
GN Name=KTR2; OrderedLocusNames=YKR061W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RX PubMed=8256512; DOI=10.1002/yea.320091004;
RA Lussier M., Camirand A., Sdicu A.-M., Bussey H.;
RT "KTR2: a new member of the KRE2 mannosyltransferase gene family.";
RL Yeast 9:1057-1063(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
CC -!- FUNCTION: Involved in N-linked glycosylation. Transfers an alpha-D-
CC mannosyl residue from GDP-mannose into lipid-linked oligosaccharide,
CC forming an alpha-(1->2)-D-mannosyl-D-mannose linkage.
CC -!- PATHWAY: Protein modification; protein glycosylation.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane; Single-pass type II
CC membrane protein.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 15 family.
CC {ECO:0000305}.
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DR EMBL; L17083; AAA16119.1; -; Unassigned_DNA.
DR EMBL; Z28286; CAA82140.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09212.1; -; Genomic_DNA.
DR PIR; S38193; S38193.
DR RefSeq; NP_012987.3; NM_001179851.3.
DR AlphaFoldDB; P33550; -.
DR SMR; P33550; -.
DR BioGRID; 34192; 106.
DR IntAct; P33550; 1.
DR MINT; P33550; -.
DR STRING; 4932.YKR061W; -.
DR CAZy; GT15; Glycosyltransferase Family 15.
DR iPTMnet; P33550; -.
DR MaxQB; P33550; -.
DR PaxDb; P33550; -.
DR PRIDE; P33550; -.
DR EnsemblFungi; YKR061W_mRNA; YKR061W; YKR061W.
DR GeneID; 853935; -.
DR KEGG; sce:YKR061W; -.
DR SGD; S000001769; KTR2.
DR VEuPathDB; FungiDB:YKR061W; -.
DR eggNOG; KOG4472; Eukaryota.
DR GeneTree; ENSGT00940000176287; -.
DR HOGENOM; CLU_024327_4_2_1; -.
DR InParanoid; P33550; -.
DR OMA; CPSAYYM; -.
DR BioCyc; YEAST:G3O-32029-MON; -.
DR UniPathway; UPA00378; -.
DR PRO; PR:P33550; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P33550; protein.
DR GO; GO:0005794; C:Golgi apparatus; IDA:SGD.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000026; F:alpha-1,2-mannosyltransferase activity; IBA:GO_Central.
DR GO; GO:0000030; F:mannosyltransferase activity; IDA:SGD.
DR GO; GO:0000032; P:cell wall mannoprotein biosynthetic process; IMP:SGD.
DR GO; GO:0097502; P:mannosylation; IBA:GO_Central.
DR GO; GO:0006487; P:protein N-linked glycosylation; IMP:SGD.
DR GO; GO:0006493; P:protein O-linked glycosylation; IBA:GO_Central.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR002685; Glyco_trans_15.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR31121; PTHR31121; 1.
DR Pfam; PF01793; Glyco_transf_15; 1.
DR PIRSF; PIRSF018153; Glyco_trans_15; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Glycosyltransferase; Golgi apparatus; Membrane;
KW Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..425
FT /note="Probable mannosyltransferase KTR2"
FT /id="PRO_0000208243"
FT TOPO_DOM 1..13
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 14..33
FT /note="Helical; Signal-anchor for type II membrane protein"
FT TOPO_DOM 34..425
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REGION 34..89
FT /note="Stem region"
FT /evidence="ECO:0000250"
FT REGION 90..425
FT /note="Catalytic"
FT /evidence="ECO:0000250"
FT ACT_SITE 313
FT /note="Nucleophile"
FT /evidence="ECO:0000255"
FT CARBOHYD 65
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 81
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 92
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 167
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 425 AA; 50651 MW; CF48E73E5BB0F832 CRC64;
MQICKVFLTQ VKKLLFVSLL FCLIAQTCWL ALVPYQRQLS LDSYFFRRSR EVSSRYDFTR
RRHMNQTLKL SSNTYNDEPL NKTKGIKNQR ENATLLMLVR NWELSGALRS MRSLEDRFNK
NYQYDWTFLN DVPFDQEFIE ATTAMASGRT QYALIPAEDW NRPSWINETL FEEALQLMEE
KNILYGGSKS YRNMCRFNSG FFFRQKILDP YDFYFRVEPD VEYFCDFPYD PFKVMRQNNK
KYGFVITMYE YEDTIPSLWE AVEEYLEETE SADIDMESNA FGFVSNFDFI GKSFGVIDSN
SGYNLCHFWT NFEIGDLNFF RSEKYIRFFE YLDSKGGFYY ERWGDAPVHS IAASLLLKKD
EIIHFDELGY KHMPFGTCPS AYYLRLQQRC LCDSNHPDNI DLNVISCLRR WWKDGSGKYF
LKHDS
