KTR7_YEAST
ID KTR7_YEAST Reviewed; 517 AA.
AC P40504; D6VVK1;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=Probable mannosyltransferase KTR7;
DE EC=2.4.1.-;
GN Name=KTR7; OrderedLocusNames=YIL085C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169870;
RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL Nature 387:84-87(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP CHARACTERIZATION.
RX PubMed=9090056;
RX DOI=10.1002/(sici)1097-0061(19970315)13:3<267::aid-yea72>3.0.co;2-k;
RA Lussier M., Sdicu A.-M., Winnett E., Vo D.H., Sheraton J., Duesterhoeft A.,
RA Storms R.K., Bussey H.;
RT "Completion of the Saccharomyces cerevisiae genome sequence allows
RT identification of KTR5, KTR6 and KTR7 and definition of the nine-membered
RT KRE2/MNT1 mannosyltransferase gene family in this organism.";
RL Yeast 13:267-274(1997).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Possible glycosyltransferase that transfers an alpha-D-
CC mannosyl residue from GDP-mannose into lipid-linked oligosaccharide,
CC forming an alpha-(1->2)-D-mannosyl-D-mannose linkage.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type II
CC membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 2890 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 15 family.
CC {ECO:0000305}.
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DR EMBL; Z46728; CAA86709.1; -; Genomic_DNA.
DR EMBL; BK006942; DAA08467.1; -; Genomic_DNA.
DR PIR; S49795; S49795.
DR RefSeq; NP_012181.3; NM_001179433.3.
DR AlphaFoldDB; P40504; -.
DR SMR; P40504; -.
DR BioGRID; 34907; 50.
DR DIP; DIP-4677N; -.
DR IntAct; P40504; 3.
DR MINT; P40504; -.
DR STRING; 4932.YIL085C; -.
DR CAZy; GT15; Glycosyltransferase Family 15.
DR MaxQB; P40504; -.
DR PaxDb; P40504; -.
DR PRIDE; P40504; -.
DR EnsemblFungi; YIL085C_mRNA; YIL085C; YIL085C.
DR GeneID; 854724; -.
DR KEGG; sce:YIL085C; -.
DR SGD; S000001347; KTR7.
DR VEuPathDB; FungiDB:YIL085C; -.
DR eggNOG; KOG4472; Eukaryota.
DR GeneTree; ENSGT00940000176287; -.
DR HOGENOM; CLU_024327_2_0_1; -.
DR InParanoid; P40504; -.
DR OMA; YQHSTIQ; -.
DR BioCyc; YEAST:YIL085C-MON; -.
DR PRO; PR:P40504; -.
DR Proteomes; UP000002311; Chromosome IX.
DR RNAct; P40504; protein.
DR GO; GO:0005794; C:Golgi apparatus; ISS:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000026; F:alpha-1,2-mannosyltransferase activity; IBA:GO_Central.
DR GO; GO:0000030; F:mannosyltransferase activity; ISS:SGD.
DR GO; GO:0000032; P:cell wall mannoprotein biosynthetic process; IBA:GO_Central.
DR GO; GO:0031505; P:fungal-type cell wall organization; IMP:SGD.
DR GO; GO:0097502; P:mannosylation; IBA:GO_Central.
DR GO; GO:0006487; P:protein N-linked glycosylation; ISS:SGD.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR002685; Glyco_trans_15.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR31121; PTHR31121; 1.
DR Pfam; PF01793; Glyco_transf_15; 2.
DR PIRSF; PIRSF018153; Glyco_trans_15; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Glycosyltransferase; Membrane; Reference proteome;
KW Signal-anchor; Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..517
FT /note="Probable mannosyltransferase KTR7"
FT /id="PRO_0000208248"
FT TOPO_DOM 1..23
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 24..44
FT /note="Helical; Signal-anchor for type II membrane protein"
FT TOPO_DOM 45..517
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REGION 45..85
FT /note="Stem region"
FT /evidence="ECO:0000250"
FT REGION 86..517
FT /note="Catalytic"
FT /evidence="ECO:0000250"
FT ACT_SITE 367
FT /note="Nucleophile"
FT /evidence="ECO:0000255"
FT CARBOHYD 89
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 144
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 517 AA; 61438 MW; 3E02FA8AA5456DEF CRC64;
MAIRLNPKVR RFLLDKCRQK RYGFLFLGCI FAILYCMGTW PFFAKDIVHD PNNLPYSLQD
YSTDKDEPFF RGCTDTKLYL QNPAYSKMNA SFVMLTRNEE IEDVLKTMRS IEGHFNKWFK
YPYVFLNDDP FTDHFKDQIQ AATNATVEFG TVDEIMWEFP AKVRNSLQFK ASLEDQNDRG
IMYGNMESYH KMCRFYSGIF YKHPLVSKYE WYWRIEPDVD FFCDISYDPF FEMAKHNKKY
GFTVLITELY WTVPNLFRTT KSFIKKTAGL KENLGTLWKL FTFNYNILDT DDEEISRWVN
FPWDAKPKLT EKLMVDFLLE NHGQVNNEED LEGIQYLVER ARSKVPMLED SLEGEDYNLC
HFWSNFEIAR VDLFDNEIYN AYFKFLEESG GFWTERWGDA PIHSIGLGMT LDLEDVHYFR
DIGYRHSSLQ HCPKNALQSQ ENLNTFDEGY NFGCGCRCVC PKKGEDIEDH STPCMDIFFE
LLHGREYEKE FPGCYKPSIK DKDVIEEIRR ENFRVIE
