KTRC_BACSU
ID KTRC_BACSU Reviewed; 221 AA.
AC P39760;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Ktr system potassium uptake protein C;
DE Short=K(+)-uptake protein KtrC;
DE AltName: Full=ORF4;
GN Name=ktrC; Synonyms=ykqB, ylxV, yzaC; OrderedLocusNames=BSU14510;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RA Kobayashi K., Sato T., Kobayashi Y.;
RT "The nucleotide sequence analysis of kinC region.";
RL Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969500; DOI=10.1099/13500872-142-11-3033;
RA Winters P., Caldwell R.M., Enfield L., Ferrari E.;
RT "The ampS-nprE (124 degrees-127 degrees) region of the Bacillus subtilis
RT 168 chromosome: sequencing of a 27 kb segment and identification of several
RT genes in the area.";
RL Microbiology 142:3033-3037(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=12562800; DOI=10.1128/jb.185.4.1289-1298.2003;
RA Holtmann G., Bakker E.P., Uozumi N., Bremer E.;
RT "KtrAB and KtrCD: two K+ uptake systems in Bacillus subtilis and their role
RT in adaptation to hypertonicity.";
RL J. Bacteriol. 185:1289-1298(2003).
CC -!- FUNCTION: Catalytic subunit of the KtrCD potassium uptake transporter.
CC The 2 major potassium transporter complexes KtrAB and KtrCD confer
CC resistance to both suddenly imposed and prolonged osmotic stress.
CC {ECO:0000269|PubMed:12562800}.
CC -!- SUBUNIT: Homodimer, tetramer (dimer of homodimer) and octamer (tetramer
CC of homodimer). Part of the KtrCD complex formed by an octameric
CC catalytic ring of KtrC and a membrane associated dimer of KtrD forming
CC a potassium channel (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane
CC protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Impaired potassium uptake.
CC {ECO:0000269|PubMed:12562800}.
CC -!- SIMILARITY: Belongs to the KtrA potassium transport family.
CC {ECO:0000305}.
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DR EMBL; D37799; BAA07051.1; -; Genomic_DNA.
DR EMBL; AF012285; AAC24926.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13324.1; -; Genomic_DNA.
DR PIR; A69862; A69862.
DR RefSeq; NP_389334.1; NC_000964.3.
DR RefSeq; WP_003222311.1; NZ_JNCM01000035.1.
DR PDB; 6I8V; X-ray; 1.99 A; A=1-221.
DR PDBsum; 6I8V; -.
DR AlphaFoldDB; P39760; -.
DR SMR; P39760; -.
DR STRING; 224308.BSU14510; -.
DR TCDB; 2.A.38.4.4; the k(+) transporter (trk) family.
DR jPOST; P39760; -.
DR PaxDb; P39760; -.
DR PRIDE; P39760; -.
DR EnsemblBacteria; CAB13324; CAB13324; BSU_14510.
DR GeneID; 64303339; -.
DR GeneID; 939429; -.
DR KEGG; bsu:BSU14510; -.
DR PATRIC; fig|224308.179.peg.1581; -.
DR eggNOG; COG0569; Bacteria.
DR InParanoid; P39760; -.
DR OMA; PEYEAGC; -.
DR PhylomeDB; P39760; -.
DR BioCyc; BSUB:BSU14510-MON; -.
DR PRO; PR:P39760; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008324; F:cation transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.1450; -; 1.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR006037; RCK_C.
DR InterPro; IPR036721; RCK_C_sf.
DR InterPro; IPR003148; RCK_N.
DR Pfam; PF02080; TrkA_C; 1.
DR Pfam; PF02254; TrkA_N; 1.
DR SUPFAM; SSF116726; SSF116726; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS51202; RCK_C; 1.
DR PROSITE; PS51201; RCK_N; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Ion transport; Membrane; NAD; Potassium;
KW Potassium transport; Reference proteome; Transport.
FT CHAIN 1..221
FT /note="Ktr system potassium uptake protein C"
FT /id="PRO_0000049610"
FT DOMAIN 4..126
FT /note="RCK N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00543"
FT DOMAIN 135..219
FT /note="RCK C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00544"
FT BINDING 12
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 32..34
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 52..53
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 74..76
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 99..101
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 105
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 121
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT STRAND 5..8
FT /evidence="ECO:0007829|PDB:6I8V"
FT HELIX 12..23
FT /evidence="ECO:0007829|PDB:6I8V"
FT STRAND 29..33
FT /evidence="ECO:0007829|PDB:6I8V"
FT HELIX 35..40
FT /evidence="ECO:0007829|PDB:6I8V"
FT TURN 41..44
FT /evidence="ECO:0007829|PDB:6I8V"
FT STRAND 45..50
FT /evidence="ECO:0007829|PDB:6I8V"
FT HELIX 56..61
FT /evidence="ECO:0007829|PDB:6I8V"
FT STRAND 68..72
FT /evidence="ECO:0007829|PDB:6I8V"
FT HELIX 78..90
FT /evidence="ECO:0007829|PDB:6I8V"
FT STRAND 94..99
FT /evidence="ECO:0007829|PDB:6I8V"
FT HELIX 103..111
FT /evidence="ECO:0007829|PDB:6I8V"
FT STRAND 115..118
FT /evidence="ECO:0007829|PDB:6I8V"
FT HELIX 120..133
FT /evidence="ECO:0007829|PDB:6I8V"
FT STRAND 137..142
FT /evidence="ECO:0007829|PDB:6I8V"
FT STRAND 144..153
FT /evidence="ECO:0007829|PDB:6I8V"
FT TURN 156..160
FT /evidence="ECO:0007829|PDB:6I8V"
FT HELIX 163..166
FT /evidence="ECO:0007829|PDB:6I8V"
FT HELIX 168..172
FT /evidence="ECO:0007829|PDB:6I8V"
FT STRAND 175..181
FT /evidence="ECO:0007829|PDB:6I8V"
FT STRAND 184..188
FT /evidence="ECO:0007829|PDB:6I8V"
FT STRAND 200..206
FT /evidence="ECO:0007829|PDB:6I8V"
FT HELIX 207..217
FT /evidence="ECO:0007829|PDB:6I8V"
SQ SEQUENCE 221 AA; 24348 MW; 31F94403CB58B2F6 CRC64;
MKKEFAVIGL GRFGGSICKA LSEEGVEVMA MDIDEDKVNE YAKIASHAVI GDSTDESVLK
NLGLRNFDHV IVAIGENIQA SILTTLILKE LGVHTITVKA QNDYHEKVLS KIGADHIVHP
ERDMAKRIAH NIVSNNVLDY LELSEEHSLV EIVANSRLAG NTLLDLDIRA KYGINIVAIK
RGKEVIVSPL ATEVIHQEDI LIVIGSVTDI SRFEKRVLHT K
