ID   ARCA_PSEPK              Reviewed;         417 AA.
AC   Q88P52;
DT   03-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Arginine deiminase {ECO:0000255|HAMAP-Rule:MF_00242};
DE            Short=ADI {ECO:0000255|HAMAP-Rule:MF_00242};
DE            EC=3.5.3.6 {ECO:0000255|HAMAP-Rule:MF_00242};
DE   AltName: Full=Arginine dihydrolase {ECO:0000255|HAMAP-Rule:MF_00242};
DE            Short=AD {ECO:0000255|HAMAP-Rule:MF_00242};
GN   Name=arcA {ECO:0000255|HAMAP-Rule:MF_00242}; OrderedLocusNames=PP_1001;
OS   Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS   / KT2440).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=160488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX   PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA   Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA   Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA   Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA   Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA   Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA   Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA   Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA   Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT   "Complete genome sequence and comparative analysis of the metabolically
RT   versatile Pseudomonas putida KT2440.";
RL   Environ. Microbiol. 4:799-808(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + L-arginine = L-citrulline + NH4(+);
CC         Xref=Rhea:RHEA:19597, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:32682, ChEBI:CHEBI:57743; EC=3.5.3.6;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00242};
CC   -!- PATHWAY: Amino-acid degradation; L-arginine degradation via ADI
CC       pathway; carbamoyl phosphate from L-arginine: step 1/2.
CC       {ECO:0000255|HAMAP-Rule:MF_00242}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00242}.
CC   -!- SIMILARITY: Belongs to the arginine deiminase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00242}.
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DR   EMBL; AE015451; AAN66626.1; -; Genomic_DNA.
DR   RefSeq; NP_743162.1; NC_002947.4.
DR   RefSeq; WP_010952186.1; NC_002947.4.
DR   AlphaFoldDB; Q88P52; -.
DR   SMR; Q88P52; -.
DR   STRING; 160488.PP_1001; -.
DR   PRIDE; Q88P52; -.
DR   EnsemblBacteria; AAN66626; AAN66626; PP_1001.
DR   KEGG; ppu:PP_1001; -.
DR   PATRIC; fig|160488.4.peg.1064; -.
DR   eggNOG; COG2235; Bacteria.
DR   HOGENOM; CLU_052662_0_0_6; -.
DR   OMA; SAWIYDG; -.
DR   PhylomeDB; Q88P52; -.
DR   BioCyc; PPUT160488:G1G01-1074-MON; -.
DR   BRENDA; 3.5.3.6; 5092.
DR   UniPathway; UPA00254; UER00364.
DR   Proteomes; UP000000556; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016990; F:arginine deiminase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019547; P:arginine catabolic process to ornithine; IEA:UniProtKB-UniPathway.
DR   GO; GO:0018101; P:protein citrullination; IEA:GOC.
DR   HAMAP; MF_00242; Arg_deiminase; 1.
DR   InterPro; IPR003876; Arg_deiminase.
DR   PIRSF; PIRSF006356; Arg_deiminase; 1.
DR   PRINTS; PR01466; ARGDEIMINASE.
DR   TIGRFAMs; TIGR01078; arcA; 1.
PE   3: Inferred from homology;
KW   Arginine metabolism; Cytoplasm; Hydrolase; Reference proteome.
FT   CHAIN           1..417
FT                   /note="Arginine deiminase"
FT                   /id="PRO_0000182226"
FT   ACT_SITE        405
FT                   /note="Amidino-cysteine intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00242"
SQ   SEQUENCE   417 AA;  46462 MW;  10EE002A1742326B CRC64;
     MSAEKQKYGV HSEAGKLRKV MVCAPGLAHK RLTPSNCDEL LFDDVIWVDQ AKRDHFDFVT
     KMRERGVDVL EMHNLLTDIV QNKEALKWIL DRKITPDTVG VGLTNEVRSW LEGLEPRHLA
     EFLIGGVAGQ DLPQSEGADV VKMYNDYLGH SSFILPPLPN TQFTRDTTCW IYGGVTLNPM
     YWPARRQETL LTTAIYKFHK EFTEADFQVW YGDPDKDHGN ATLEGGDVMP IGNGIVLIGM
     GERTSRQAIG QLAQNLFAKG AVKEVIVAGL PKSRAAMHLD TVFSFCDRDL VTVFPEVVKE
     IVPFIIRPDE SKPYGMDVRR INKSFIEVVG EQLGVQLRVV ETGGNSFAAE REQWDDGNNV
     VAIEPGVVIG YDRNTYTNTL LRKAGIEVIT ISAGELGRGR GGGHCMTCPI VRDPINY