ID   KU2_SACEN               Reviewed;         306 AA.
AC   A4FG06;
DT   24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   17-APR-2007, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Non-homologous end joining protein Ku 2 {ECO:0000255|HAMAP-Rule:MF_01875};
GN   Name=ku2 {ECO:0000255|HAMAP-Rule:MF_01875}; OrderedLocusNames=SACE_3709;
OS   Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 /
OS   NBRC 13426 / NCIMB 8594 / NRRL 2338).
OC   Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
OC   Saccharopolyspora.
OX   NCBI_TaxID=405948;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL
RC   2338;
RX   PubMed=17369815; DOI=10.1038/nbt1297;
RA   Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S.,
RA   Haydock S.F., Leadlay P.F.;
RT   "Complete genome sequence of the erythromycin-producing bacterium
RT   Saccharopolyspora erythraea NRRL23338.";
RL   Nat. Biotechnol. 25:447-453(2007).
CC   -!- FUNCTION: With LigD forms a non-homologous end joining (NHEJ) DNA
CC       repair enzyme, which repairs dsDNA breaks with reduced fidelity. Binds
CC       linear dsDNA with 5'- and 3'- overhangs but not closed circular dsDNA
CC       nor ssDNA. Recruits and stimulates the ligase activity of LigD.
CC       {ECO:0000255|HAMAP-Rule:MF_01875}.
CC   -!- SUBUNIT: Homodimer. Interacts with LigD. {ECO:0000255|HAMAP-
CC       Rule:MF_01875}.
CC   -!- SIMILARITY: Belongs to the prokaryotic Ku family. {ECO:0000255|HAMAP-
CC       Rule:MF_01875}.
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DR   EMBL; AM420293; CAM02981.1; -; Genomic_DNA.
DR   RefSeq; WP_011874120.1; NZ_PDBV01000001.1.
DR   AlphaFoldDB; A4FG06; -.
DR   SMR; A4FG06; -.
DR   STRING; 405948.SACE_3709; -.
DR   EnsemblBacteria; CAM02981; CAM02981; SACE_3709.
DR   KEGG; sen:SACE_3709; -.
DR   eggNOG; COG1273; Bacteria.
DR   HOGENOM; CLU_048975_1_1_11; -.
DR   OMA; IRYRKVC; -.
DR   OrthoDB; 1724780at2; -.
DR   Proteomes; UP000006728; Chromosome.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:UniProtKB-UniRule.
DR   CDD; cd00789; KU_like; 1.
DR   Gene3D; 2.40.290.10; -; 1.
DR   HAMAP; MF_01875; Prokaryotic_Ku; 1.
DR   InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR   InterPro; IPR009187; Prok_Ku.
DR   InterPro; IPR016194; SPOC-like_C_dom_sf.
DR   PANTHER; PTHR41251; PTHR41251; 1.
DR   Pfam; PF02735; Ku; 1.
DR   PIRSF; PIRSF006493; Prok_Ku; 1.
DR   SMART; SM00559; Ku78; 1.
DR   SUPFAM; SSF100939; SSF100939; 1.
DR   TIGRFAMs; TIGR02772; Ku_bact; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; DNA-binding; Reference proteome.
FT   CHAIN           1..306
FT                   /note="Non-homologous end joining protein Ku 2"
FT                   /id="PRO_0000389195"
FT   DOMAIN          21..206
FT                   /note="Ku"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01875"
FT   REGION          233..306
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        238..254
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   306 AA;  35112 MW;  A1054780902DFCA2 CRC64;
     MINSPPIGYL PGMRTMWRGA ISFGLVNIGV RLYAATADHD YQFHQVHRQD HGRIHHKRVC
     EECGREIDYD DIVKGYETSD GELVVLDSQD LRKLPIRTDR AIDLLECVPA EQVDPTYFQK
     TYYLEPEKSA RRPYVLLREA LRRTDLLAVV KITMRQRETL ATIRPAGDVL VLHTMLWPDE
     IRRPDFDFLT DDAGDTEVSK QEIDMATSLV ENMTEDFDPT EFTDDYQRAL AELIDAKTKG
     TKPPRKRPEP ADEGEVVDLM GALERSVDQA RTSRTSRRKT TASASRRRSS SNREKTGSRD
     KTRKRA