KU70_ORYSJ
ID KU70_ORYSJ Reviewed; 624 AA.
AC Q7F1M0; A0A0N7KN15; A3BH97; B9FVV9; Q7F1L9;
DT 18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=ATP-dependent DNA helicase 2 subunit KU70;
DE Short=OsKU70;
DE EC=3.6.4.12;
DE AltName: Full=ATP-dependent DNA helicase 2 subunit 1;
DE AltName: Full=ATP-dependent DNA helicase II 70 kDa subunit;
GN Name=KU70; OrderedLocusNames=Os07g0184900, LOC_Os07g08729;
GN ORFNames=OJ1046_F10.128, OsJ_23357, OsJ_23359;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP TISSUE SPECIFICITY, INTERACTION WITH KU80, AND DISRUPTION PHENOTYPE.
RX PubMed=19923234; DOI=10.1104/pp.109.150391;
RA Hong J.P., Byun M.Y., An K., Yang S.J., An G., Kim W.T.;
RT "OsKu70 is associated with developmental growth and genome stability in
RT rice.";
RL Plant Physiol. 152:374-387(2010).
CC -!- FUNCTION: Single-stranded DNA-dependent ATP-dependent helicase.
CC Involved in DNA non-homologous end joining (NHEJ) required for double-
CC strand break repair. When associated with KU70, binds to double-
CC stranded telomeric and non-telomeric DNA sequences, but not to single-
CC stranded DNA (By similarity). Required for the maintenance of
CC chromosome stability and normal developmental growth. Plays a role in
CC maintaining telomere length. Acts as a negative regulator in telomere
CC homeostasis. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- SUBUNIT: Interacts with KU80. {ECO:0000269|PubMed:19923234}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed ubiquitously.
CC {ECO:0000269|PubMed:19923234}.
CC -!- DOMAIN: The N-terminal domain (1-265) is necessary for binding to KU80.
CC -!- DISRUPTION PHENOTYPE: Severe developmental defects in both vegetative
CC and reproductive organs resulting in sterile flowers. Hypersensitivity
CC to methylmethane sulfonate (MMS). Longer telomeres.
CC {ECO:0000269|PubMed:19923234}.
CC -!- SIMILARITY: Belongs to the ku70 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC83190.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=EAZ38936.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=EEE66696.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP003861; BAC83189.1; -; Genomic_DNA.
DR EMBL; AP003861; BAC83190.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008213; BAF20975.1; -; Genomic_DNA.
DR EMBL; AP014963; BAT00354.1; -; Genomic_DNA.
DR EMBL; CM000144; EAZ38936.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CM000144; EEE66696.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AK102066; BAG95371.1; -; mRNA.
DR RefSeq; XP_015646738.1; XM_015791252.1.
DR AlphaFoldDB; Q7F1M0; -.
DR SMR; Q7F1M0; -.
DR STRING; 4530.OS07T0184900-01; -.
DR PaxDb; Q7F1M0; -.
DR PRIDE; Q7F1M0; -.
DR EnsemblPlants; Os07t0184900-01; Os07t0184900-01; Os07g0184900.
DR GeneID; 4342584; -.
DR Gramene; Os07t0184900-01; Os07t0184900-01; Os07g0184900.
DR KEGG; osa:4342584; -.
DR eggNOG; KOG2327; Eukaryota.
DR HOGENOM; CLU_014815_2_0_1; -.
DR InParanoid; Q7F1M0; -.
DR OMA; PNDMMGI; -.
DR OrthoDB; 402798at2759; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000007752; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR ExpressionAtlas; Q7F1M0; baseline and differential.
DR Genevisible; Q7F1M0; OS.
DR GO; GO:0043564; C:Ku70:Ku80 complex; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:EnsemblPlants.
DR GO; GO:0042162; F:telomeric DNA binding; IBA:GO_Central.
DR GO; GO:0071480; P:cellular response to gamma radiation; IBA:GO_Central.
DR GO; GO:0071481; P:cellular response to X-ray; IBA:GO_Central.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IMP:UniProtKB.
DR GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IBA:GO_Central.
DR GO; GO:0009408; P:response to heat; IEA:EnsemblPlants.
DR GO; GO:0000723; P:telomere maintenance; IMP:UniProtKB.
DR Gene3D; 1.10.720.30; -; 1.
DR Gene3D; 2.40.290.10; -; 1.
DR Gene3D; 3.40.50.410; -; 1.
DR Gene3D; 4.10.970.10; -; 1.
DR InterPro; IPR006165; Ku70.
DR InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR InterPro; IPR027388; Ku70_bridge/pillars_dom_sf.
DR InterPro; IPR005160; Ku_C.
DR InterPro; IPR005161; Ku_N.
DR InterPro; IPR003034; SAP_dom.
DR InterPro; IPR036361; SAP_dom_sf.
DR InterPro; IPR016194; SPOC-like_C_dom_sf.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR12604:SF2; PTHR12604:SF2; 1.
DR Pfam; PF02735; Ku; 1.
DR Pfam; PF03730; Ku_C; 1.
DR Pfam; PF03731; Ku_N; 1.
DR Pfam; PF02037; SAP; 1.
DR PIRSF; PIRSF003033; Ku70; 1.
DR SMART; SM00559; Ku78; 1.
DR SMART; SM00513; SAP; 1.
DR SUPFAM; SSF100939; SSF100939; 1.
DR SUPFAM; SSF53300; SSF53300; 1.
DR SUPFAM; SSF68906; SSF68906; 1.
DR TIGRFAMs; TIGR00578; ku70; 1.
DR PROSITE; PS50800; SAP; 1.
PE 1: Evidence at protein level;
KW ATP-binding; DNA damage; DNA recombination; DNA repair; DNA-binding;
KW Helicase; Hydrolase; Nucleotide-binding; Nucleus; Reference proteome.
FT CHAIN 1..624
FT /note="ATP-dependent DNA helicase 2 subunit KU70"
FT /id="PRO_0000394139"
FT DOMAIN 270..470
FT /note="Ku"
FT DOMAIN 588..622
FT /note="SAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00186"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 624 AA; 70056 MW; A3A56D1E1980B65E CRC64;
MDLDPEGLFR DDSDEDDDNV QEREANKEMV VYLIDASPKM FTPATKADEK EETHFHTIVN
CITHALKTQI IGRSYDEVAI CFFNTKEKKN LQELAGVYVY NVTEREPLDR PDARLIKEFS
CIEDSFMSNI GSRYGITSGS RENTLYNALW VAQALLRKGS VKTVSKRIVI FTNEDDPFGG
LTGAVKTDMI RTTIQRARDA QDLGLSIELL PLSRPDEEFN MSLFYADLIG LEGDEIVDYL
PSSGEKLEDM TNQLKKRMMK KRKVKTLAFA ITNDVCIEVN TYALIRSTTP GAITWLDSIS
NLPLKAERSF ICNDTGALIQ DPQKRFQVYN DKIVKFSTRE LSDVKRVSSH HLRLLGFKPL
DYLKDYHNLR PSTFIYPSDE QIFGSTRVFV ALHSSMRRLG RFALAFYGNP TRPQLVALIA
QEEVTSAGGQ IEPPGIHMIY LPYSDDVRYP EEVHLTSDDA PRATDEQIKK ASNLLRRIDL
KNFSVCQFSN PALQRHYGIL EALALGEDEM PDVKDETLPD EEGLARPVVV KAVEEFKASV
YGENYDQEEA EAAAAKAGAS KKRKALTDAA AEKSAAHNWA ELADTGKLKD MTVVDLKSYL
SAHGLPVSGK KEALVSRILT HLGK
