ID   KU80_ASPFU              Reviewed;         725 AA.
AC   Q4WI96;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=ATP-dependent DNA helicase II subunit 2;
DE            EC=3.6.4.12;
DE   AltName: Full=ATP-dependent DNA helicase II subunit Ku80;
GN   Name=ku80; ORFNames=AFUA_2G02620;
OS   Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS   A1100) (Aspergillus fumigatus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=330879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX   PubMed=16372009; DOI=10.1038/nature04332;
RA   Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA   Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA   Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA   Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA   Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA   Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA   Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA   Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA   Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA   Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA   O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA   Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA   Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA   Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA   Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA   Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA   Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA   Barrell B.G., Denning D.W.;
RT   "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT   Aspergillus fumigatus.";
RL   Nature 438:1151-1156(2005).
CC   -!- FUNCTION: Single-stranded DNA-dependent ATP-dependent helicase.
CC       Involved in non-homologous end joining (NHEJ) DNA double strand break
CC       repair. DNA-binding is sequence-independent but has a high affinity to
CC       nicks in double-stranded DNA and to the ends of duplex DNA. Binds to
CC       naturally occurring chromosomal ends, and therefore provides
CC       chromosomal end protection. Required also for telomere recombination to
CC       repair telomeric ends in the absence of telomerase. ku70, of the
CC       ku70/ku80 heterodimer, binds to the stem loop of tlc1, the RNA
CC       component of telomerase. Involved in telomere maintenance. Interacts
CC       with telomeric repeats and subtelomeric sequences thereby controlling
CC       telomere length and protecting against subtelomeric rearrangement.
CC       Maintains telomeric chromatin, which is involved in silencing the
CC       expression of genes located at the telomere. Required for mating-type
CC       switching (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SUBUNIT: Heterodimer of Ku70 and Ku80. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, telomere
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ku80 family. {ECO:0000305}.
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DR   EMBL; AAHF01000008; EAL87359.1; -; Genomic_DNA.
DR   RefSeq; XP_749397.1; XM_744304.1.
DR   AlphaFoldDB; Q4WI96; -.
DR   SMR; Q4WI96; -.
DR   STRING; 746128.CADAFUBP00001925; -.
DR   EnsemblFungi; EAL87359; EAL87359; AFUA_2G02620.
DR   GeneID; 3506885; -.
DR   KEGG; afm:AFUA_2G02620; -.
DR   VEuPathDB; FungiDB:Afu2g02620; -.
DR   eggNOG; KOG2326; Eukaryota.
DR   HOGENOM; CLU_010975_1_1_1; -.
DR   InParanoid; Q4WI96; -.
DR   OMA; WAMQYVW; -.
DR   OrthoDB; 598957at2759; -.
DR   Proteomes; UP000002530; Chromosome 2.
DR   GO; GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
DR   GO; GO:0043564; C:Ku70:Ku80 complex; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0042162; F:telomeric DNA binding; IBA:GO_Central.
DR   GO; GO:0071480; P:cellular response to gamma radiation; IBA:GO_Central.
DR   GO; GO:0071481; P:cellular response to X-ray; IBA:GO_Central.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IMP:AspGD.
DR   GO; GO:0000723; P:telomere maintenance; IBA:GO_Central.
DR   CDD; cd00873; KU80; 1.
DR   Gene3D; 1.25.40.240; -; 1.
DR   Gene3D; 2.40.290.10; -; 1.
DR   Gene3D; 3.40.50.410; -; 1.
DR   InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR   InterPro; IPR024193; Ku80.
DR   InterPro; IPR036494; Ku_C_sf.
DR   InterPro; IPR005161; Ku_N.
DR   InterPro; IPR014893; Ku_PK_bind.
DR   InterPro; IPR016194; SPOC-like_C_dom_sf.
DR   InterPro; IPR002035; VWF_A.
DR   InterPro; IPR036465; vWFA_dom_sf.
DR   Pfam; PF02735; Ku; 1.
DR   Pfam; PF03731; Ku_N; 1.
DR   Pfam; PF08785; Ku_PK_bind; 1.
DR   PIRSF; PIRSF016570; Ku80; 1.
DR   SMART; SM00559; Ku78; 1.
DR   SUPFAM; SSF100939; SSF100939; 1.
DR   SUPFAM; SSF101420; SSF101420; 1.
DR   SUPFAM; SSF53300; SSF53300; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chromosome; DNA damage; DNA recombination; DNA repair;
KW   DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW   Reference proteome; Telomere.
FT   CHAIN           1..725
FT                   /note="ATP-dependent DNA helicase II subunit 2"
FT                   /id="PRO_0000278348"
FT   DOMAIN          229..476
FT                   /note="Ku"
FT   REGION          267..290
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   725 AA;  81747 MW;  629EB6AFD83910E7 CRC64;
     MAEKEATVYI VDVGKSMGEK RNGRSMTDLE WAMQYVWDCI TATVATGRKT ATIGVIGLKT
     DETSNELDDD PHFKHISVFT EIQQFLMPDI RRLGALVKPS ETDKGDAISA IILAIQMIVT
     YCKKLKYKRK IVLVTNGQGW MSNEDLDQIT KKIKEDNIDL VVLGTDFDDP EYGFKEEGKD
     PRKAENEALL RGLVEDCNGA FGTLEQAVSE MDIPRAKRVR TVATFKGFLQ LGNPEEYDTA
     LRIPVERYYR TYVAKPPSAS SFVLRSDVGA EGEQGESSQL PKSPPEGGAL TSVRNLRTYE
     VPDENAPGGK FDVEREELAK GYEYGRTAVH ISETDENITT LETYAALELV GFIQTDKYDR
     YMHMSTTNII IGQRGNDKAS LALSSFIHAL FELECYAVAR LVVKENKPPV MVLLAPSIEP
     DYECLLEVQL PFAEDVRTYR FPPLDKVITV SGKVVTEHRN LPNKDLLDAM SEYVDSMELV
     DTDETGNPVE GLPIDDSFSP VLHRIDSAIR YRAIHPDDPV LPPSKNLTKL SQPPQDLVEK
     SKAYLQKLME AADVKKVPPK VKGRKRIRES EKPLSGLDVD ALLHQEKRAR ISPTNAIPEF
     KQTLTQAETI ETIKDAVKQM TSIIEDQIKN SLGDANYDRV IEEMGVMREE LISFEEPALY
     NDFLRQLKDK LLKEELGGDR RELWWLLRRS QLGLINQRES DQSDVTEEQA KEVSLRCHLS
     SRGIY