KU80_CHAGB
ID KU80_CHAGB Reviewed; 736 AA.
AC Q2H6C5;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 25-MAY-2022, entry version 79.
DE RecName: Full=ATP-dependent DNA helicase II subunit 2;
DE EC=3.6.4.12;
DE AltName: Full=ATP-dependent DNA helicase II subunit Ku80;
GN Name=KU80; ORFNames=CHGG_05790;
OS Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 /
OS NRRL 1970) (Soil fungus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Chaetomiaceae; Chaetomium.
OX NCBI_TaxID=306901;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970;
RX PubMed=25720678; DOI=10.1128/genomea.00021-15;
RA Cuomo C.A., Untereiner W.A., Ma L.-J., Grabherr M., Birren B.W.;
RT "Draft genome sequence of the cellulolytic fungus Chaetomium globosum.";
RL Genome Announc. 3:E0002115-E0002115(2015).
CC -!- FUNCTION: Single-stranded DNA-dependent ATP-dependent helicase.
CC Involved in non-homologous end joining (NHEJ) DNA double strand break
CC repair. DNA-binding is sequence-independent but has a high affinity to
CC nicks in double-stranded DNA and to the ends of duplex DNA. Binds to
CC naturally occurring chromosomal ends, and therefore provides
CC chromosomal end protection. Required also for telomere recombination to
CC repair telomeric ends in the absence of telomerase. KU70, of the
CC KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA
CC component of telomerase. Involved in telomere maintenance. Interacts
CC with telomeric repeats and subtelomeric sequences thereby controlling
CC telomere length and protecting against subtelomeric rearrangement.
CC Maintains telomeric chromatin, which is involved in silencing the
CC expression of genes located at the telomere. Required for mating-type
CC switching (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- SUBUNIT: Heterodimer of Ku70 and Ku80. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, telomere
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ku80 family. {ECO:0000305}.
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DR EMBL; CH408031; EAQ89171.1; -; Genomic_DNA.
DR RefSeq; XP_001221885.1; XM_001221884.1.
DR AlphaFoldDB; Q2H6C5; -.
DR SMR; Q2H6C5; -.
DR STRING; 38033.XP_001221885.1; -.
DR EnsemblFungi; EAQ89171; EAQ89171; CHGG_05790.
DR GeneID; 4390172; -.
DR eggNOG; KOG2326; Eukaryota.
DR HOGENOM; CLU_010975_1_1_1; -.
DR InParanoid; Q2H6C5; -.
DR OMA; WAMQYVW; -.
DR OrthoDB; 598957at2759; -.
DR Proteomes; UP000001056; Unassembled WGS sequence.
DR GO; GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0043564; C:Ku70:Ku80 complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0042162; F:telomeric DNA binding; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:InterPro.
DR GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR CDD; cd00873; KU80; 1.
DR Gene3D; 1.25.40.240; -; 1.
DR Gene3D; 2.40.290.10; -; 1.
DR Gene3D; 3.40.50.410; -; 1.
DR InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR InterPro; IPR024193; Ku80.
DR InterPro; IPR036494; Ku_C_sf.
DR InterPro; IPR005161; Ku_N.
DR InterPro; IPR014893; Ku_PK_bind.
DR InterPro; IPR016194; SPOC-like_C_dom_sf.
DR InterPro; IPR036465; vWFA_dom_sf.
DR Pfam; PF02735; Ku; 1.
DR Pfam; PF03731; Ku_N; 1.
DR Pfam; PF08785; Ku_PK_bind; 1.
DR PIRSF; PIRSF016570; Ku80; 1.
DR SMART; SM00559; Ku78; 1.
DR SUPFAM; SSF100939; SSF100939; 1.
DR SUPFAM; SSF101420; SSF101420; 1.
DR SUPFAM; SSF53300; SSF53300; 1.
PE 3: Inferred from homology;
KW ATP-binding; Chromosome; DNA damage; DNA recombination; DNA repair;
KW DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW Reference proteome; Telomere.
FT CHAIN 1..736
FT /note="ATP-dependent DNA helicase II subunit 2"
FT /id="PRO_0000278351"
FT DOMAIN 232..479
FT /note="Ku"
FT REGION 261..286
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 268..282
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 736 AA; 81825 MW; 95686D901D696725 CRC64;
MADKEATVYI VDLGESMADC HNGRDESDLD FGMRYVWDKI STTVAASRKT WTLGVVGLNT
EETNNAQDSE GLEGYEHISV LQDIGPMTMT QLRELRSSIQ TSHTYGGDAI SGIVVALAMI
ELFTKKLKYN RRIILVTNGE SPIDDESSED VANRLNYSNI ELIVIGVDFD DADYGFKEED
KSKGKARNEK TLRKLVEQCN NGVFGTMQQA VEELAIPRIK PVRPFKAYDG ALTLGDPEKY
KSALSIHVER YFKTKRAPAP PASTVVVNSE PGGASQSETL NEDTEMGDAE FSGVKHMRTY
RVNDPDAPGG KRDVEFEDLA KGYQYGRTVV PFSESDFSIT KLETKKSFTI LGFIPFSSYN
PFLNLGETGI VVAQKHNEEA ELGLSALIHA LHELESYAVA RYVQKDGAQP QLVLLKPNPA
IEDDFECLYD VPLPFAEDLR SYQFPPLDKV LTVTGNIIKE HRLLPSEDLK QAMSDFVDAM
DLSGFDVDED GKPTEYAPID ETYNPTIHRM NQAIRARAVD PDAPIKPPAE ILLRFSKPPE
KLIEKAKPEI EALIDAAEIK KVPAKAQGRG SRKEPVKPLS GLDIDSLLGE SKRTTISLDN
AVPEFKQILA TAADDATIES AAKQMGQIVR KLIQDSFADL FYARAAENLR VMREELISLE
VPGLYNKFLT GLKKSILSGE LDGDRREMWF KHIVGGHLSL ITQDESEVSE VTAEEAKALC
AWSRPNITYR YFRDTA
