ID   KU80_ORYSJ              Reviewed;         688 AA.
AC   Q75IP6; B9F7Z5; C7J053; Q75IP5;
DT   18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=ATP-dependent DNA helicase 2 subunit KU80;
DE            EC=3.6.4.12;
DE   AltName: Full=ATP-dependent DNA helicase 2 subunit 2;
DE   AltName: Full=ATP-dependent DNA helicase II 80 kDa subunit;
GN   Name=KU80; OrderedLocusNames=Os03g0856200, LOC_Os03g63920;
GN   ORFNames=OsJ_13430;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   INTERACTION WITH KU70.
RX   PubMed=19923234; DOI=10.1104/pp.109.150391;
RA   Hong J.P., Byun M.Y., An K., Yang S.J., An G., Kim W.T.;
RT   "OsKu70 is associated with developmental growth and genome stability in
RT   rice.";
RL   Plant Physiol. 152:374-387(2010).
CC   -!- FUNCTION: Single-stranded DNA-dependent ATP-dependent helicase.
CC       Involved in DNA non-homologous end joining (NHEJ) required for double-
CC       strand break repair. When associated with KU70, binds to double-
CC       stranded telomeric and non-telomeric DNA sequences, but not to single-
CC       stranded DNA. Plays a role in maintaining telomere length. Acts as a
CC       negative regulator of telomerase (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SUBUNIT: Interacts with KU70. {ECO:0000269|PubMed:19923234}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ku80 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAS01978.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAH92449.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=EEE60332.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC128647; AAS01977.1; -; Genomic_DNA.
DR   EMBL; AC128647; AAS01978.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; DP000009; ABF99985.1; -; Genomic_DNA.
DR   EMBL; AP008209; BAH92449.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014959; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CM000140; EEE60332.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_015631136.1; XM_015775650.1.
DR   AlphaFoldDB; Q75IP6; -.
DR   SMR; Q75IP6; -.
DR   STRING; 4530.OS03T0856200-04; -.
DR   iPTMnet; Q75IP6; -.
DR   PaxDb; Q75IP6; -.
DR   PRIDE; Q75IP6; -.
DR   GeneID; 9267125; -.
DR   KEGG; osa:9267125; -.
DR   eggNOG; KOG2326; Eukaryota.
DR   HOGENOM; CLU_010975_2_0_1; -.
DR   InParanoid; Q75IP6; -.
DR   OrthoDB; 598957at2759; -.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000007752; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   Genevisible; Q75IP6; OS.
DR   GO; GO:0043564; C:Ku70:Ku80 complex; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0042162; F:telomeric DNA binding; IBA:GO_Central.
DR   GO; GO:0071480; P:cellular response to gamma radiation; IBA:GO_Central.
DR   GO; GO:0071481; P:cellular response to X-ray; IBA:GO_Central.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IBA:GO_Central.
DR   GO; GO:0000723; P:telomere maintenance; IBA:GO_Central.
DR   CDD; cd00873; KU80; 1.
DR   Gene3D; 1.25.40.240; -; 1.
DR   Gene3D; 2.40.290.10; -; 1.
DR   Gene3D; 3.40.50.410; -; 1.
DR   InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR   InterPro; IPR024193; Ku80.
DR   InterPro; IPR005160; Ku_C.
DR   InterPro; IPR036494; Ku_C_sf.
DR   InterPro; IPR005161; Ku_N.
DR   InterPro; IPR014893; Ku_PK_bind.
DR   InterPro; IPR016194; SPOC-like_C_dom_sf.
DR   InterPro; IPR036465; vWFA_dom_sf.
DR   Pfam; PF02735; Ku; 1.
DR   Pfam; PF03730; Ku_C; 1.
DR   Pfam; PF03731; Ku_N; 1.
DR   Pfam; PF08785; Ku_PK_bind; 1.
DR   PIRSF; PIRSF016570; Ku80; 1.
DR   SMART; SM00559; Ku78; 1.
DR   SUPFAM; SSF100939; SSF100939; 1.
DR   SUPFAM; SSF101420; SSF101420; 1.
DR   SUPFAM; SSF53300; SSF53300; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; DNA damage; DNA recombination; DNA repair; DNA-binding;
KW   Helicase; Hydrolase; Nucleotide-binding; Nucleus; Reference proteome.
FT   CHAIN           1..688
FT                   /note="ATP-dependent DNA helicase 2 subunit KU80"
FT                   /id="PRO_0000394140"
FT   DOMAIN          222..430
FT                   /note="Ku"
SQ   SEQUENCE   688 AA;  77443 MW;  968742C5CB4FDE18 CRC64;
     MARNKEALVL LLDVGPSMHG VLQEVENICS TLVHKKLVYN RSDEIGVVLF GTKETSNELA
     KELGGYKHVV VARDIKVVDE ETTNALQNLP RGTSPGDFLD AIVVGLDMLI RKFGNIKGKQ
     RMCLVTDAQH PLRDPPQGTK KDQVDTIADQ MKRHEIKMDC IIFRESGVRH NAVMDENDQL
     LYHFRERSVT KVVHVDSPTS LLGALRTRNV LPVTVFRGDL EVSSSFKIKV WVYKKTSEEK
     FPTLKKYSDK APASDKFASH EVKVDYEYKS VLEPDTVVPP DQRIKGYLYG PQVVPISSAE
     WEAVKFKPEK GVKLLGFTDR SSISRHYFMK DVFSFVPEPG NTKAVAAVSA LARAMSEMNK
     VAILRCVWRQ GQGNVALGVL TPNISSAKNV LDSFYFNILP FAEDIREFQF RSFSSLPSSS
     QPTKEQQEAA DNLVKMLDLA PPGREEILKP DFTPNPMLER FYRYLDLKSK QPDANVPPLD
     KCLKKITEPD PDVIDYQAPL IKKLGNVFEL KENPKKKKAR TQDRLTYTGA DDQAKLLEEP
     SAEKVGVSEA LYPPKKKAGE IGDHNPVQDF EAMLTQRSSS TWVQTAIEEM QKYITALIQD
     SCDRDNHQKA LECLVALRKA CIIEQEPNEY NGFVTKLCQK FRPAGDKIFL QLLSSKKASL
     ISKEEAPDSD VTEEMARNFC LKPEPSSQ